2001
DOI: 10.1073/pnas.171120098
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A metallothionein containing a zinc finger within a four-metal cluster protects a bacterium from zinc toxicity

Abstract: Zinc is essential for many cellular processes, including DNA synthesis, transcription, and translation, but excess can be toxic. A zinc-induced gene, smtA, is required for normal zinc-tolerance in the cyanobacterium Synechococcus PCC 7942. Here we report that the protein SmtA contains a cleft lined with Cys-sulfur and His-imidazole ligands that binds four zinc ions in a Zn4Cys9His2 cluster. The thiolate sulfurs of five Cys ligands provide bridges between the two ZnCys 4 and two ZnCys3His sites, giving two fuse… Show more

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Cited by 172 publications
(195 citation statements)
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“…Fig. 3 here (single column) << A relatively high -sheet content could be alternatively achieved by multiple shorter -sheet stretches as similarly found in the 15 amino acids long linker region of a bacterial MT [13].…”
Section: 1mentioning
confidence: 73%
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“…Fig. 3 here (single column) << A relatively high -sheet content could be alternatively achieved by multiple shorter -sheet stretches as similarly found in the 15 amino acids long linker region of a bacterial MT [13].…”
Section: 1mentioning
confidence: 73%
“…From two 3D structures we know that also one or more Cys residues can be replaced by His residues without alterations of the coordination number or geometry [13,14]. Two basic metal-thiolate structures are known: The M II 4 Cys 11 cluster of the -domain of vertebrate and echinoderm MTs [15][16][17][18][19][20][21][22][23] and the M II 3 Cys 9 cluster of the -domain of vertebrate, crustacean, and echinoderm MTs (Fig.…”
Section: Metal Ion Contentsmentioning
confidence: 99%
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“…in the M II 4 Cys 9 His 2 cluster found in bacterial MTs (A). [54] the case of Cd 6 E c -1 cross peaks between the two most intense signals were found (659-661 ppm) originating from the two 113 Cd 2+ ions of the γ-domain (Fig. 6B) Cdcysteine coupling follows in many cases a Karplus-type dependence and therefore can be used to determine the H β -C β -S-Cd dihedral angles important for structure calculation.…”
Section: Plant Metallothioneinsmentioning
confidence: 99%
“…Zinc fingers are common DNA binding motifs in eukaryotes where they usually occur in multiples and have a role in genetic regulation (Rhodes and Klug, 1993). In the last few years, putative zinc finger motifs have been observed more frequently in prokaryotic sequences and some of these motifs have been shown by chemical analysis to coordinate zinc (Makarova et al, 2002;Blindauer et al, 2002;Blindauer et al, 2001;Chou et al, 1998). In order to better understand the mechanism of TnpA target site selection the role of the putative zinc finger motif was investigated.…”
Section: Introductionmentioning
confidence: 99%