A metagenomic assessment of viral contamination on fresh parsley plants irrigated with fecally tainted river water

Fernández-Cassi, Xavier; Timoneda, Natàlia; Gonzales-Gustavson, Eloy; Bofill-Mas, Sı́lvia; Gironés, Rosina

doi:10.1016/j.ijfoodmicro.2017.06.001

Cited by 32 publications

(28 citation statements)

References 63 publications

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“…Metatranscriptomic sequencing has reached a virus detection limit similar to diagnostic qPCR when liquid biological samples are being used, such as blood 61,62 , nasopharyngeal swabs 63,64 or clarified cell culture supernatant 65 . However, when complex samples such as sewage 1,66 or plant tissue 67 are used, metatranscriptomic sequencing is considerably less sensitive. Despite this reduced sensitivity, it is important to note metatranscriptomic sequencing can detect multiple regions, if not the entire virus genome (Fig.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity and specificity of metatranscriptomics as an arbovirus surveillance tool

Batovska

Mee

Lynch

et al. 2019

Sci Rep

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be established for routine use of metatranscriptomic sequencing. The criterion for positive detection of a virus established in this paper is one example of a process that can be applied to produce comparable results, which also accounts for potential contamination found in the negative control. Further work utilising wild caught mosquitoes from diverse populations will help to establish metatranscriptomic sequencing as a tool that can broaden the capabilities of arbovirus surveillance.

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Section: Discussionmentioning

confidence: 99%

Sensitivity and specificity of metatranscriptomics as an arbovirus surveillance tool

Batovska

Mee

Lynch

et al. 2019

Sci Rep

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“…Irrigation water was the main suspect as the origin of contamination. A metagenomic assessment of the viral contamination of fresh parsley plants in Spain demonstrated the presence of HEV-3f [ 142 ]. Among samples from fresh lettuce points of sale, HEV prevalence was 3.2% (4/125) [ 143 ].…”

Section: Different Routes Of Transmissionmentioning

confidence: 99%

Hepatitis E in High-Income Countries: What Do We Know? And What Are the Knowledge Gaps?

Capai

Charrel

Falchi

2018

Viruses

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Hepatitis E virus (HEV) is a positive-strand RNA virus transmitted by the fecal–oral route. HEV genotypes 1 and 2 infect only humans and cause mainly waterborne outbreaks. HEV genotypes 3 and 4 are widely represented in the animal kingdom, and are mainly transmitted as a zoonosis. For the past 20 years, HEV infection has been considered an imported disease in developed countries, but now there is evidence that HEV is an underrecognized pathogen in high-income countries, and that the incidence of confirmed cases has been steadily increasing over the last decade. In this review, we describe current knowledge about the molecular biology of HEV, its clinical features, its main routes of transmission, and possible therapeutic strategies in developed countries.

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“…Contaminated water may also lead to contamination of food. For example, human adenoviruses were recently detected on fresh parsley using a metagenomic approach [47] and are often contaminating shellfish [48]. No zoonotic transmission has been reported as adenoviruses are usually speciesspecific [49].…”

Section: Human Adenovirusmentioning

confidence: 99%

Selected Viruses Detected on and in our Food

Bachofen

2018

Curr Clin Micro Rpt

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Purpose of Review The purpose of this review is to provide an update on recent literature and findings concerning selected foodborne viruses. Two groups of viruses were selected: (a) the most important viruses contaminating food, based on numbers of publications in the last 5 years and (b) viruses infecting sources of food that might have an impact on human health. Recent Findings Important foodborne viruses such as norovirus, hepatitis A and rotavirus are usually "only" contaminating food and are detected on the surface of foodstuffs. However, they are threats to human public health and make up for the majority of cases. In contrast, the meaning of viruses born from within the food such as natural animal and plant viruses is still in many cases unknown. An exception is Hepatitis E virus that is endemic in pigs, transmitted via pork meat and is recognised as an emerging zoonosis in industrialised countries. Summary Even though the clinical meaning of "new" foodborne viruses, often detected by next generation sequencing, still needs clarification, the method has great potential to enhance surveillance and detection particularly in view of an increasingly globalised food trade.

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A metagenomic assessment of viral contamination on fresh parsley plants irrigated with fecally tainted river water

Cited by 32 publications

References 63 publications

Sensitivity and specificity of metatranscriptomics as an arbovirus surveillance tool

Sensitivity and specificity of metatranscriptomics as an arbovirus surveillance tool

Hepatitis E in High-Income Countries: What Do We Know? And What Are the Knowledge Gaps?

Selected Viruses Detected on and in our Food

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