A metabolomics study of ascorbic acid-induced<i>in situ</i>freezing tolerance in spinach (<i>Spinacia oleracea</i>L.)

Arora, Rajeev; Min, Kyungwon; Chen, Keting

doi:10.1101/2020.01.23.916973

Cited by 3 publications

(3 citation statements)

References 62 publications

(81 reference statements)

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“…In previous studies, researchers have revealed that cold exposure results in a variety of alterations in physiology and gene expression patterns, and thousands of genes were even identified that were up-and downregulated in response to cold, including the metabolism of carbohydrates, molecular chaperones, antifreeze proteins, signal transduction (receptor kinase, protein kinase/phosphatase, Ca 2+ binding protein) and regulatory proteins [6][7][8][9][10]. In addition, transcription factors could regulate gene expression to improve yields under adverse growing conditions [7,11].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars

Zhou

Zhu

et al. 2020

BMC Plant Biol

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Background: Low temperature is a major factor influencing the growth and development of Chinese jujube (Ziziphus jujuba Mill.) in cold winter and spring. Little is known about the molecular mechanisms enabling jujube to cope with different freezing stress conditions. To elucidate the freezing-related molecular mechanism, we conducted comparative transcriptome analysis between 'Dongzao' (low freezing tolerance cultivar) and 'Jinsixiaozao' (high freezing tolerance cultivar) using RNA-Seq. Results: More than 20,000 genes were detected at chilling (4°C) and freezing (− 10°C, − 20°C, − 30°C and − 40°C) stress between the two cultivars. The numbers of differentially expressed genes (DEGs) between the two cultivars were 1831, 2030, 1993, 1845 and 2137 under the five treatments. Functional enrichment analysis suggested that the metabolic pathway, response to stimulus and catalytic activity were significantly enriched under stronger freezing stress. Among the DEGs, nine participated in the Ca 2+ signal pathway, thirty-two were identified to participate in sucrose metabolism, and others were identified to participate in the regulation of ROS, plant hormones and antifreeze proteins. In addition, important transcription factors (WRKY, AP2/ERF, NAC and bZIP) participating in freezing stress were activated under different degrees of freezing stress. Conclusions: Our research first provides a more comprehensive understanding of DEGs involved in freezing stress at the transcriptome level in two Z. jujuba cultivars with different freezing tolerances. These results may help to elucidate the molecular mechanism of freezing tolerance in jujube and also provides new insights and candidate genes for genetically enhancing freezing stress tolerance.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars

Zhou

Zhu

et al. 2020

BMC Plant Biol

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“…Stress mitigation practices have shown positive effects on stress tolerance. Application of ascorbic acid has increased the activity levels of APX, CAT, and SOD in spinach ( Spinacia oleracea L.), resulting in increased freezing tolerance (Min et al., 2020), and the application of salicylic acid has improved regrowth of KBG after exposure to heat stress (He et al., 2005). Thus, future research could investigate the rate and timing of exogenous applications of ascorbic acid or salicylic acid to enhance a turf's ability to withstand traffic stress.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant enzyme responses of Kentucky bluegrass to simulated athletic traffic stress

Pease

Thoms

Arora

et al. 2021

Intl Turfgrass Soc Res J

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Increased athletic field usage and growing understanding of injury prevention have sports turf managers interested in implementing management practices that will improve turfgrass traffic stress tolerance. Previous research has shown that other abiotic stressors, such as mowing/rolling, high/low temperature, and drought, result in changes to turfgrass antioxidant enzyme activity levels. This study was conducted to determine how simulated athletic traffic affects the magnitude and duration of changes in antioxidant enzyme activity of Kentucky bluegrass (Poa pratensis L.; KBG). Two levels of simulated athletic traffic (0 and 2 games week −1 ) were applied to a blend of 'True Blue HGT' KBG using a modified Baldree traffic simulator for 7 simulated traffic events (STEs) in each year coinciding with the 2018 and 2019 fall football schedule. One STE of two games wk −1 represents a high school field hosting junior varsity and varsity games each week. Experimental design was a split-plot randomized complete block with four replications. Turfgrass leaves were individually cut from sub-plots at 0, 2, 4, 8, 12, and 24 h after simulated traffic application to assay the activities of ascorbate peroxidase (APX), catalase (CAT), and superoxide dismutase (SOD). Traffic application resulted in decreased activity of APX by 30-45% in 2019, CAT by 26-28% in 2019, and SOD by 3-13% on some rating dates in 2018 and 2019. Activity of APX was lowest at 4-12 h after STE. Catalase and SOD activity was highest at 0 or 4 h after STEs in early 2019 but in late 2019 it was highest at 12 or 24 h after STEs. These results suggest that cumulative traffic stress increases the time required for antioxidant enzyme activity, directing future research to investigate timings and frequency of product application, potentially conferring protection to antioxidant enzymes.

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“…In previous studies, researchers have revealed that cold exposure results in a variety of alterations in physiology and gene expression patterns, and thousands of genes were even identi ed that were up-and downregulated in response to cold, including the metabolism of carbohydrates, molecular chaperones, antifreeze proteins, signal transduction (receptor kinase, protein kinase/phosphatase, Ca 2+ binding protein) and regulatory proteins [6][7][8][9][10]. In addition, transcription factors could regulate gene expression to improve yields under adverse growing conditions [7,11].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars

Zhou

Zhu

et al. 2020

Preprint

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Background: Low temperature is a major factor influencing the growth and development of Chinese jujube (Ziziphus jujuba Mill.) in cold winter and spring. Little is known about the molecular mechanisms enabling jujube to cope with different freezing stress conditions. To elucidate the freezing-related molecular mechanism, we conducted comparative transcriptome analysis between ‘Dongzao’ (low freezing tolerance cultivar) and ‘Jinsixiaozao’ (high freezing tolerance cultivar) using RNA-Seq. Results: More than 20,000 genes were detected at chilling (4°C) and freezing (-10°C, -20°C, -30°C and -40°C) stress between the two cultivars. The numbers of differentially expressed genes (DEGs) between the two cultivars were 1831, 2030, 1993, 1845 and 2137 under the five treatments. Functional enrichment analysis suggested that the metabolic pathway, response to stimulus and catalytic activity were significantly enriched under stronger freezing stress. Among the DEGs, nine participated in the Ca2+ signal pathway, thirty-two were identified to participate in sucrose metabolism, and others were identified to participate in the regulation of ROS, plant hormones and antifreeze proteins. In addition, important transcription factors (WRKY, AP2/ERF, NAC and bZIP) participating in freezing stress were activated under different degrees of freezing stress. Conclusions: Our research first provides a more comprehensive understanding of DEGs involved in freezing stress at the transcriptome level in two Z. jujuba cultivars with different freezing tolerances. These results may help to elucidate the molecular mechanism of freezing tolerance in jujube and also provides new insights and candidate genes for genetically enhancing freezing stress tolerance.

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A metabolomics study of ascorbic acid-inducedin situfreezing tolerance in spinach (Spinacia oleraceaL.)

Cited by 3 publications

References 62 publications

Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars

Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars

Antioxidant enzyme responses of Kentucky bluegrass to simulated athletic traffic stress

Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars

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