A metabolomics approach to evaluate the effects of shiitake mushroom (Lentinula edodes) treatment in undernourished young rats

Molz, Patrí­cia; Ellwanger, Joel Henrique; Santos, Carla Eliete Iochims dos; Dias, Johnny Ferraz; Campos, Deivis de; Corbellini, Valeriano Antônio; Prá, Daniel; Putzke, Marisa Terezinha Lopes; Franke, Silvia Isabel Rech

doi:10.1016/j.nimb.2013.05.108

Cited by 7 publications

(8 citation statements)

References 23 publications

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“…This result is repeated when this comparison is made with the control group (Fig. 3B-D), with the exception of the DI Table 1 Se Intake by animals in each group and its relation to the water group, LD 50 50 of sodium selenite in rats (value range between 3,000 and 12,000 mg Se/kg bw) [22]. y The human RDA is 55 mg Se/d, which corresponds to 0.79 mg Se/kg based on a 70-kg adult [30].…”

Section: Dna Damagementioning

confidence: 88%

“…Nevertheless, recommendation of larger amounts of the mineral than those tested in this study has yet to be investigated by other studies that should also evaluate the effects of such doses at the cellular and tissue levels as well as investigating the quantities of Se in various organs through the application of accurate techniques for metabolomics analysis such as Particle-induced X-ray emission [50], an approach that is currently being used by our research group. It is also very important to note that the conditions of PD evaluated in this study are experimental, and differ with regard to various parameters from those found in PD as it occurs in humans.…”

Section: Discussionmentioning

confidence: 99%

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Selenium reduces bradykinesia and DNA damage in a rat model of Parkinson's disease

et al. 2015

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Section: Dna Damagementioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Selenium reduces bradykinesia and DNA damage in a rat model of Parkinson's disease

et al. 2015

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“…00 . We used the BMI because anthropometrics is a simpler and efficient method to assess nutritional status in either animals (Novelli et al 2007, Molz et al 2014 or humans (WHO 1995, De Onis and Habicht 1996, Hedley et al 2004.…”

Section: Measurements Of Chow and Water Intakementioning

confidence: 99%

“…PATRÍCIA MOLZ et al is characterized by a decrease in the metabolic rate due to a lack of nutrients that are important to life (Molz et al 2014), resulting from reduced nutrient intake (Schilp et al 2011). Nutritional deficiency may affect several essential metabolic processes and can interfere with genome stability (Mendoza-Núñez et al 2005).…”

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confidence: 99%

Recognition memory and DNA damage in undernourished young rats

Molz

Ellwanger

Zenkner

et al. 2016

An. Acad. Bras. Ciênc.

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This study evaluated the recognition memory and the levels of DNA damage (blood and hippocampus) in undernourished young Wistar rats. The experiment was conducted along 14-week with rodents divided in control group (CG, n=8) and undernourished group (UG, n=12) which was submitted to caloric restriction. Nutritional status for undernutrition was defined by Body Mass Index (BMI) ≤0.45g/cm 2 and by weighting the organs/tissue (liver, spleen, intestine, peritoneal fat, kidney and encephalon). The Novel Object Recognition Test assessed recognition memory and the Comet Assay evaluated the levels of DNA damage. Student t test, 2-way ANOVA and Pearson's correlation analysis were used and the significance level was of p<0.05. The UG showed lower BMI and organ/tissue weights than CG (p<0.001). In shortterm memory, the recognition rate was higher in the UG (p<0.05), only after 4 weeks. In the long-term memory, again recognition rate was higher in the UG than the CG, after 4 weeks (p<0.001) and 14 weeks (p<0.01). The UG showed decreased levels of DNA damage in the blood (p<0.01) and increased levels in the hippocampus (p<0.01). We concluded in this study that the undernutrition by caloric restriction did not cause impairment in recognition memory, however induced DNA damage in the hippocampus.

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“…It is constantly evolving and taking advantage of new developments in analytical chemistry, including analytical techniques, instrumentation, analytical software, statistical methods, or computational techniques to accelerate or improve data collection, data analysis, and data interpretation. The primary analytical technologies used in metabolomics include liquid chromatography coupled with single-stage mass spectrometry (LC-MS) [1,2,3] or tandem mass spectrometry (LC-MS/MS) [4,5,6,7,8,9], gas chromatography coupled to mass spectrometry (GC-MS) [1,2,10,11], high or ultrahigh performance liquid chromatography coupled to UV or fluorescent detection (HPLC/UPLC) [12,13,14,15,16,17,18,19], and nuclear magnetic resonance (NMR) spectroscopy [20,21,22,23,24,25,26,27]. Each analytical platform has its own advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

NMR Spectroscopy for Metabolomics Research

et al. 2019

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Over the past two decades, nuclear magnetic resonance (NMR) has emerged as one of the three principal analytical techniques used in metabolomics (the other two being gas chromatography coupled to mass spectrometry (GC-MS) and liquid chromatography coupled with single-stage mass spectrometry (LC-MS)). The relative ease of sample preparation, the ability to quantify metabolite levels, the high level of experimental reproducibility, and the inherently nondestructive nature of NMR spectroscopy have made it the preferred platform for long-term or large-scale clinical metabolomic studies. These advantages, however, are often outweighed by the fact that most other analytical techniques, including both LC-MS and GC-MS, are inherently more sensitive than NMR, with lower limits of detection typically being 10 to 100 times better. This review is intended to introduce readers to the field of NMR-based metabolomics and to highlight both the advantages and disadvantages of NMR spectroscopy for metabolomic studies. It will also explore some of the unique strengths of NMR-based metabolomics, particularly with regard to isotope selection/detection, mixture deconvolution via 2D spectroscopy, automation, and the ability to noninvasively analyze native tissue specimens. Finally, this review will highlight a number of emerging NMR techniques and technologies that are being used to strengthen its utility and overcome its inherent limitations in metabolomic applications.

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A metabolomics approach to evaluate the effects of shiitake mushroom (Lentinula edodes) treatment in undernourished young rats

Cited by 7 publications

References 23 publications

Selenium reduces bradykinesia and DNA damage in a rat model of Parkinson's disease

Selenium reduces bradykinesia and DNA damage in a rat model of Parkinson's disease

Recognition memory and DNA damage in undernourished young rats

NMR Spectroscopy for Metabolomics Research

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