A metabolomic study of preterm human and formula milk by high resolution NMR and GC/MS analysis: preliminary results

Marincola, Flaminia Cesare; Noto, Antonio; Caboni, Pierluigi; Reali, Alessandra; Barberini, Luigi; Lussu, Milena; Murgia, Federica; Santoru, Maria Laura; Atzori, Luigi; Fanos, Vassilios

doi:10.3109/14767058.2012.715436

Cited by 95 publications

(87 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Finally, metabolomics analysis is also applicable to food itself to identify major nutritional constituents and/or particular metabolites showing significant bioactivity, including milk, cereals, wine, fruits, vegetables, and meat (13). This is of particular relevance for the analysis of breast milk (14) in order to explore the relationship among mother's nutrition, breastfeeding, and child's nutrition for optimal growth and development. Human milk varies in composition, time, and volume, and is the first source of nutrient for the newborn.…”

Section: Metabolomics Technologies In Clinical Studiesmentioning

confidence: 99%

Metabolomics perspectives in pediatric research

et al. 2013

View full text Add to dashboard Cite

Increasing evidence points toward the critical and long-term involvement of prenatal and early nutrition and lifestyle on later health and disease risk predisposition. Metabolomics is now a well-established top-down systems biology approach that explores the genetic-environment-health paradigm. The generalization of such approaches has opened new research areas to deepen our current understanding of many physiological processes, as well as foods and nutrient functionalities in target populations. It is envisioned that this will provide new avenues toward preventive medicine and prognostic strategies for tailored therapeutic and personalized nutrition management. The development of systems biology approaches and the new generation of biomarker patterns will provide the opportunity to associate complex metabolic regulations with the etiology of multifactorial pediatric diseases. This may subsequently lead to the development of system mechanistic hypotheses that could be targeted with new nutritional personalized concepts. Therefore, this review aims to describe recent applications of metabolomics in preclinical and clinical fields with insights into disease diagnostics/monitoring and improvement of homeostasis metabolic regulation that may be translatable to novel therapeutic and nutrition advances in pediatric research. Over the past decades, awareness about the role of nutrition and lifestyle in health and disease risk management has increased, with key emphasis on the prevention of metabolic disorders, including cardiometabolic diseases and type 2 diabetes (1-3). In parallel, increasing evidence points toward the critical and long-term involvement of early nutrition and lifestyle on later health and disease risk predisposition (4). It therefore becomes pertinent to look at newborn metabolism, development, and nutritional requirements to understand the onset of child and adult physiological conditions. Pregnancy, childbirth, lactation, and human milk composition are influenced by the intake of foods (macro-and micronutrients), which subsequently determine both the short-and long-term health and nutritional outcomes of an individual (5). Therefore, it is essential to build knowledge not only on the metabolic dysregulations but also on the effects of specific foods to comprehensively document the metabolic processes associated with individual health at the different stages of the life cycle (Figure 1). This will allow us to formulate nutritional solutions to improve or circumvent certain metabolic imbalances.The generalization of systems biology approaches has opened new research areas to further our current understanding of many physiological processes, as well as the functionalities of foods and nutrients. In particular, metabolomics is recognized as a powerful top-down systems biology approach to understand the genetic-environment-health paradigm and to identify clinically relevant biomarkers. Its application in clinical studies is envisioned to shed new light on the regulatory processes of complex mammalian sy...

show abstract

Section: Metabolomics Technologies In Clinical Studiesmentioning

confidence: 99%

Metabolomics perspectives in pediatric research

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The application of metabonomics analysis is well suited for neonatal metabolic research due to the simple, safe, and noninvasive approach that can be deployed on urine and stool samples (21,24). In particular, promising metabolic insights into urine (20) and also into human breast milk (25) have led to a rapid expansion of the field of clinical applications of metabolomics in neonatology (26). Such data will serve as a reference to provide understanding of metabolic processes involved in developmental physiology, metabolic programming, and nutritional requirements.…”

mentioning

confidence: 99%

Impact of breast-feeding and high- and low-protein formula on the metabolism and growth of infants from overweight and obese mothers

et al. 2013

View full text Add to dashboard Cite

Background:The combination of maternal obesity in early pregnancy and high protein intake in infant formula feeding might predispose to obesity risk in later life. Methods: This study assesses the impact of breast-or formula-feeding (differing in protein content by 1.65 or 2.7 g/100 kcal) on the metabolism of term infants from overweight and obese mothers. From birth to 3 mo of age, infants received exclusively either breast-or starter formula-feeding and until 6 mo, exclusively either a formula designed for this study or breast-feeding. From 6 to 12 mo, infants received complementary weaning food. Metabonomics was conducted on the infants' urine and stool samples collected at the age of 3, 6, and 12 mo. results: Infant formula-feeding resulted in higher proteinderived short-chain fatty acids and amino acids in stools. Urine metabonomics revealed a relationship between bacterial processing of dietary proteins and host protein metabolism stimulated with increasing protein content in the formula. Moreover, formula-fed infants were metabolically different from breast-fed infants, at the level of lipid and energy metabolism (carnitines, ketone bodies, and Krebs cycle). conclusion: Noninvasive urine and stool metabolic monitoring of responses to early nutrition provides relevant readouts to assess nutritional requirements for infants' growth. c oncurring with the obesity epidemic, maternal obesity is rising (1), with subsequent increased risk of childhood overweight and obesity for the offspring of overweight and obese women (2,3). This increasing mother/child metabolic disease risk is driven by both genetics and pre/postnatal environmental factors (4). Since childhood overweight is a strong predictor of adult obesity, increased infant metabolic disease may translate into severe health consequences for the same subjects in adulthood (5,6). Although causality is not established, rapid growth in infancy is associated with later overweight and obesity (3,7). Therefore, any measure that could reduce the risk of later obesity would be of particular importance for infants born to overweight mothers, including reduction in rapid weight gain in infancy.Optimal prenatal nutrition can reduce the risk of obesity in adults by influencing birth weight (5). Moreover, breast-feeding and introduction of complementary foods adapted to both metabolic and nutritional requirements of growing infants are also envisioned as preventive measures against obesity later in life (5). Alteration of nutrition in a critical period of development in early life can affect the subsequent pattern of growth and development of tissues and organs and may predispose individuals to several disorders later in life (8,9). Interestingly, in the study conducted by Li et al. (10), breast-feeding seems to reduce the risk of being overweight during childhood in infants from obese mothers. On the other hand, obese women have difficulties in initiating and maintaining breast-feeding (11,12) likely because of defects in their hormonal response to suckling (11,12), b...

show abstract

“…Three of these studies are summarized in Table 1. Using high-resolution ¹H-NMR and GC-MS, Marincola et al (2012) identified differences in metabolite composition between pre-term human BM and infant formula milk (FM). In this study, lactose was found to be higher in pre-term BM compared to FM.…”

Section: Recent Investigations Into the Breastmilk Metabolomementioning

confidence: 99%

Breastmilk Metabolomics: Bridging the Gap between Maternal Nutrition and Infant Health Outcomes

Sumayao

Benigno

Jaen

et al. 2017

KIMIKA

View full text Add to dashboard Cite

Breastmilk (BM) is the primary source of nutrition for the newborn infant and its first six months of life. Although the importance of BM in the proper growth and development of infants has previously been recognized in various research studies, it was not until various metabolites were probed in BM that researchers discovered the various beneficial effects of BM beyond its nutritive value. Metabolomics has emerged as a discipline which aims to comprehensively profile various metabolites in food and biological fluids. Although still in its incipient stages, BM metabolomics has provided invaluable insights into the chemical interaction between mother and infant. NMR-and MS-based techniques have made it possible to explore the metabolome of BM and link it to various aspects of maternal phenotype and nutrition and breastfed infant health outcomes. In addition, recent developments in analytical approaches for BM metabolite analysis and metabolomic data analysis have allowed researchers to increase the coverage of detected metabolites using multiple platforms and have supported its functional characterization which aids in investigation of the clinical and biological importance of metabolites. These advancements can potentially aid in the development of strategies to promote healthy feeding practices for infants or novel therapeutic and nutrition advances in pediatric research.

show abstract

A metabolomic study of preterm human and formula milk by high resolution NMR and GC/MS analysis: preliminary results

Abstract: The preliminary results of this study suggest that metabolomics may provide a promising tool to study aspects related to the nutrition and health of preterm infant.

Cited by 95 publications

References 22 publications

Metabolomics perspectives in pediatric research

Metabolomics perspectives in pediatric research

Impact of breast-feeding and high- and low-protein formula on the metabolism and growth of infants from overweight and obese mothers

Breastmilk Metabolomics: Bridging the Gap between Maternal Nutrition and Infant Health Outcomes

Contact Info

Product

Resources

About