A metabolic-based approach to improve xylose utilization for fumaric acid production from acid pretreated wheat bran by Rhizopus oryzae

Using tobacco waste extract in pre-culture medium to improve xylose utilization for l-lactic acid production from cellulosic waste by Rhizopus oryzae

“…In addition, the first and second steps of pentose catabolic pathway in R. oryzae are NADPH-dependent and NAD + -dependent steps (Maas et al, 2008). Intracellular ATP concentration may be the third limiting step for conversation of xylose to L -lactic acid, because previous metabolic analysis suggested that sufficient ATP was essential for xylose metabolism (Wang et al, 2015). Yet, addition of these exogenous compounds mentioned above for production of L -lactic acid in an industrial scale is not cost-effective and almost unrealistic.…”

“…Nitrogen supply may be one of the limiting factors due to its higher requirement (approximate thrice) in xylose metabolism compared to those in glucose metabolism (Mass et al, 2008). The lack of intracellular cofactors (e.g., NADPH, NADH and NAD + ) may be the other key factor contributing to the poor xylose metabolism , because L -lactic acid is biosynthesized through the consumption of NADH (Wang et al, 2015;San et al, 2002). In addition, the first and second steps of pentose catabolic pathway in R. oryzae are NADPH-dependent and NAD + -dependent steps (Maas et al, 2008).…”

Biotechnological Transformation of Lignocellulosic Biomass in to Industrial Products: An Overview

“…These monomer sugars are utilized as carbon sources for the production of different industrial products such as ethanol, xylitol, biobutanol, bio-hydrogen, microbial polysaccharides, organic acids, and single cell proteins etc [93] [94]. Maximum laccase activity (416.4 U/ml) obtained by submerged fermentation of wheat bran by Cerrena unicolor C-139 [65] Amylase (327 IU/ml) produced by Aspergillus fumigatus NTCC1222 using wheat bran as substrate [66] Lipase activity (9.14 IU/g of dry substrate) obtained by Aspergillus niger under SSF using wheat bran as substrate [67] Lactic acid yield of 0.73 g/g substrate achieved by fermentation by Lactobacillus pentosus using wheat bran as substrate [59] Fumaric acid 20.2 g/l was achieved by fermentation by Rhizopus oryzae using wheat bran hydrolysate as substrate [58] The maximum hydrogen yield of 128.2 ml/g total volatile solid from pre-treated wheat bran by mixed anaerobic cultures [68] Wheat straw…”

Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue

“…These experiments required the enzymatic hydrolysis of pretreated lignocellulosic materials before fermentation, which is described as a separate hydrolysis and fermentation (SHF) process. In recent literature, fermentative production of fumaric acid has been carried out mainly through submerged fermentation (Liao et al 2008;Das et al 2015a;Wang et al 2015;Das et al 2016), solid state fermentation (Das et al 2015a;Das et al 2016), or immobilized submerged fermentation (Das et al 2015b). Different from recent studies, we developed a simultaneous saccharification and fermentation (SSF) process for production of fumaric acid from lignocellulose by R. oryzae.…”

“…Therefore, the cheaper, abundant, and non-edible feedstock is crucial for fermentative production of fumaric acid by Rhizopus oryzae. Some studies have focused on seeking a suitable feedstock, such as wheat bran (Wang et al 2015), pulp and paper solid waste (Das et al 2016), apple industry waste biomass (Das et al 2015a), brewery wastewater (Das et al 2015b), corn straw , and dairy manure hydrolysate (Liao et al 2008). Lignocellulosic materials (especially wood and agricultural residues) are promising potential alternatives for biorefinery characterized by renewable, cheap, abundant, and non-edible biomass.…”