A meta-analysis to determine the optimal values of precursors in the diet of dairy cows that increase the concentration of conjugated linoleic acid (CLA) in milk

Acosta-Balcazar, I. C.; Estrada-Drouaillet, B.; Bautista-Martínez, Y.; Granados-Rivera, L. D.

doi:10.22358/jafs/171458/2023

Cited by 2 publications

(1 citation statement)

References 79 publications

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“…They provide health-promoting cannabinoids (CBDs), particularly non-psychoactive CBDs, n-3 polyunsaturated fatty acids (n-3PUFA), vitamins, and essential trace elements [1][2][3][4][5]. Fortunately, both diet composition and dietary supplements (e.g., non-psychotropic CBDs and plant or fish oils rich in n-3PUFA) can increase the nutritional value of plant and animal products, thereby benefiting human health [6][7][8][9][10]. Moreover, non-psychotropic CBDs have also shown efficacy in preventing vascular diseases in humans [11].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Gas and Liquid Chromatography Methods for the Determination of Underivatised Neutral and Acidic Cannabinoids and Cholesterol

Czauderna,

Taubner,

Wojtak

2024

Molecules

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The aim of our study was to develop a gas chromatographic method coupled with mass spectrometry (GC-MS) for the determination of underivatised neutral (CBDs-N) and acidic (CBDs-A) cannabinoids (CBDs) and cholesterol (Chol). Emphasis was also placed on comparing our original GC-MS method with the currently developed C18-high-performance liquid chromatography with photodiode detection (C18-HPLC-DAD). A combination of a long GC column, shallow temperature column programme, and mass-spectrometry was employed to avoid issues arising from the overlap between CBDs and Chol and background fluctuations. The pre-column procedure for CBDs and Chol in egg yolks consisted of hexane extractions, whereas the pre-column procedure for CBDs in non-animal samples involved methanol and hexane extractions. CBDs-A underwent decarboxylation to CBDs during GC-MS analyses, and pre-column extraction of the processed sample with NaOH solution allowed for CBD-A removal. No losses of CBDs-N were observed in the samples extracted with NaOH solution. GC-MS analyses of the samples before and after extraction with NaOH solution enabled the quantification of CBDs-A and CBDs-N. CBDs-A did not undergo decarboxylation to CBDs-N during C18-HPLC-DAD runs. The use of the C18-HPLC-DAD method allowed simultaneous determination of CBDs-N and CBDs-A. In comparison to the C18-HPLC-DAD method, our GC-MS technique offered improved sensitivity, precision, specificity, and satisfactory separation of underivatised CBDs and Chol from biological materials of endogenous species, especially in hemp and hen egg yolk. The scientific novelty of the present study is the application of the GC-MS method for quantifying underivatised CBDs-A, CBDs-N, and Chol in the samples of interest.

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