A meta-analysis for assessing the contributions of trypsin and chymotrypsin as the two major endoproteases in protein hydrolysis in fish intestine

Solovyev, Mikhail; Kashinskaya, E. N.; Gisbert, Enric

doi:10.1016/j.cbpa.2023.111372

Cited by 11 publications

(6 citation statements)

References 58 publications

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“…This difference could be attributed to shape-dependent accumulation effects, as previous studies have shown that MPs accumulate differently in zebrafish guts and induce intestinal injury based on their shape (fibers > fragments > spheres) [ 22 ]. Another possible explanation is a different age of the fish, as digestive enzyme activities vary significantly during the early stages of ontogeny and can be affected by factors such as species, diet, and feeding regime [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Effect of Microplastics on the Activity of Digestive and Oxidative-Stress-Related Enzymes in Peled Whitefish (Coregonus peled Gmelin) Larvae

Frank

Интересова

Solovyev

et al. 2023

IJMS

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Microplastics (MPs) are emergent pollutants in freshwater environments and may impact aquatic organisms, including those of nutritional value. The specific activities of digestive and antioxidant enzymes can be used as good bioindicators of the potential effects of MPs on fish in case of waterborne MP contamination. In this study, we used fluorescent polystyrene microplastics (PS-MPs) to analyze the alterations in enzyme activities in larvae of Coregonus peled Gmelin (peled or Northern whitefish), one of the most valuable commercial fish species of Siberia. Our results indicate that peled larvae can ingest 2 µm PS microspheres in a waterborne exposure model. A positive correlation (rs = 0.956; p < 0.01) was found between MP concentration in water and the number of PS microspheres in fish guts, with no significant differences between 24 h and 6-day exposure groups. The ingestion of MPs caused alterations in digestive enzyme activity and antioxidant responses at the whole-body level. The presence of PS-MPs significantly stimulated (p < 0.05) the specific activity of α-Amylase and non-specific esterases in peled larvae after 24 h. However, a pronounced positive effect (p < 0.05) of MPs on the activity of pancreatic trypsine and bile salt-activated lipase was only found after 6 days of exposure compared to after 24 h. Intestinal membrane enzyme aminopeptidase N was also stimulated in the presence of PS-MPs after 6-day exposure. We also observed a significant increase in the specific activity of catalase in peled larvae after 6 days of exposure, which indicates the MP-induced modulation of oxidative stress. Taken together, these results highlight the potential impact of environmental MPs on northern commercial fish, their importance for estimating fish stocks, and the sustainability of freshwater ecosystems.

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Section: Resultsmentioning

confidence: 99%

Effect of Microplastics on the Activity of Digestive and Oxidative-Stress-Related Enzymes in Peled Whitefish (Coregonus peled Gmelin) Larvae

Frank

Интересова

Solovyev

et al. 2023

IJMS

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“…This enzyme is a proteolytic enzyme that hydrolyses the peptide bond adjacent to a free amino group. Hence, it can be inferred that leucine aminopeptidase can hydrolyze ingested proteins of mushroom meal (Solovyev et al 2023).…”

Section: Discussionmentioning

confidence: 99%

Graded levels of dietary pink oyster mushroom, Pleurotus djamor meal, affects growth, feed efficiency, lipase activity and fiber content in final whole body of Nile tilapia fingerlings, Oreochromis niloticus

Sosa¹,

Cappello-García²,

Martínez‐García³

et al. 2023

Preprint

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Expansion of aquaculture industry is evidently accompanied by an urgent necessity of aquaculture feed production. Traditionally, fish meal (FM) and soybean meal (SBM) have been the primary protein source ingredient in aquaculture diets. However, over exploitation of these commodities has conducted to their unsustainability. Hence, research of unconventional protein alternatives has emerged. Mushroom meal is one of them. To date, mushroom meals have been investigated when supplemented in low levels in aquaculture diets. Furthermore, effects of diets supplemented with mushroom meals have assessed different parameters such as, haematology, immunity, anti–bacterial & anti–oxidant activities, and heat stress. Present study, is aimed to study the effects of graded levels of dietary pink oyster mushroom (Pleurotus djamor) meal (POMM), in growth, feed efficiency, protein utilization, digestive enzymes activities and whole body proximate composition of Nile tilapia (Oreochromis niloticus) fingerlings. Experimental design included a control diet (POMM0) formulated with soybean meal, as main protein source, and four diets designed with increasing levels of POMM: 25%(POMM25); 50%(POMM50); 75%(POMM75); and 100%(POMM100). Experimental diets and final whole body were submitted to a proximate composition analysis. Growth, feed efficiency, protein utilization, and digestive enzyme activities were assessed. Compared to POMM0 and POMM25, weight gain (WG), and specific growth rate (SGR), significantly (P< 0.05) decreased in fish fed POMM50, POMM75 and POMM100%. Feed conversion ratio (FCR), protein efficiency ratio (PER) and survival rate (SR) were not significantly affected by experimental diets. Daily feed intake (DFI), and daily protein intake (DPI), decreased as POMM increased in diets. Compared to POMM0 experimental group, condition factor (K), showed a significantly higher value in fish fed POMM50, and POMM100 experimental diets. Crude fiber of final whole body of POMM100 resulted significantly higher (P< 0.05) compared to that shown in fish fed the rest of experimental diets. Acid and alkaline proteases, trypsin, chymotrypsin, leucine aminopeptidase and amylase of Nile tilapia fingerlings, were not significantly affected by experimental diets. Compared to fish fed POMM0 and POMM25 diets, experimental fish fed POMM50, POMM75, and POMM100 showed a reduction of lipase activity. In conclusion, a POMM level higher than 25% affects growth and lipase activity. While a POMM level higher than 50% affects fiber content in whole body of final fish.

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“…A post‐feeding decrease in amylase activity occurs in various fishes such as pre‐juveniles of the mullet Mugil liza (Valenciennes, 1836) in MCh (Albanesi et al, 2022), juveniles of fresh‐ and brackish‐wate r Rutilus rutilus (Linnaeus, 1758) (Abolfathi et al, 2012), adults of the marine red porgy Pagrus pagrus (Linnaeus, 1758) (Caruso et al, 2014), and the fresh‐water Siamese fighting fish Bette splendens (Regan, 1910) (Hahor et al, 2023) although with distinct timing and extent of decrease. In this context, it has been pointed out that the determination of activities of digestive enzymes of fishes requires the use of a species‐specific protocol (Solovyev et al, 2023). This can also be applied to study postprandial dynamics and the effect of irregular periods of feed restriction on the activity of digestive enzymes, which appears to be qualitatively and quantitatively different depending on habitat and dietary habit (Abolfathi et al, 2012; Albanesi et al, 2022; Caruso et al, 2014; Imsland et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Adequate levels of dietary proteins are required for normal growth and to ensure fish homeostasis (Rahman et al, 2017; Small, 2022). Trypsin, an endoprotease, is the most important protease in the digestive tract of many fishes and plays a main role in the initial steps of dietary protein digestion (Small, 2022; Solovyev et al, 2023; Steinberg, 2018). APN, a multifunctional membrane‐bound ectoprotease, plays an essential role in the final steps of protein digestion (Fairweather et al, 2012; Small, 2022; Steinberg, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Bette splendens (Regan, 1910) (Hahor et al, 2023) although with distinct timing and extent of decrease. In this context, it has been pointed out that the determination of activities of digestive enzymes of fishes requires the use of a species-specific protocol (Solovyev et al, 2023). This can also be applied to study postprandial dynamics and the effect of irregular periods of feed restriction on the activity of digestive enzymes, which appears to be qualitatively and quantitatively different depending on habitat and dietary habit (Abolfathi et al, 2012;Albanesi et al, 2022;Caruso et al, 2014;Imsland et al, 2020).…”

Section: Energy Reserves Of Juveniles Of P Orbignyanus At Distinct Ti...mentioning

confidence: 99%

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Differential modulation of digestive enzymes and energy reserves at different times after feeding in juveniles of the marine estuarine‐dependent flatfish Paralichthys orbignyanus (Valenciennes, 1839)

Albanesi,

Méndez,

Michiels

et al. 2023

Journal of Fish Biology

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Integrative studies are lacking on the responses of digestive enzymes and energy reserves in conjunction with morphological traits at distinct postprandial times in marine estuarine‐dependent flatfishes of ecological and economic importance, such as Paralichthys orbignyanus. We determined total weight (TW), hepato‐somatic index (IH), activities of digestive enzymes in the intestine, and the concentration of energy reserves in the liver and the muscle at 0, 24, 72, and 360 h after feeding in juveniles of P. orbignyanus. Amylase activity decreased at 72 h (about 30%). Maltase, sucrose, and lipase activities reached peak at 24 h (67%, 600%, and 35%, respectively). Trypsin and aminopeptidase‐N activities at 24 and 72 h, respectively, were lower than those at t = 0 (53% and 30%). A peak increase in the concentration of glycogen and triglycerides in the liver (24 h) (86% and 89%, respectively) occurred. In muscle, glycogen and triglyceride concentrations were unchanged at 24 h and higher at 72 and 360 h (100% and 60%). No changes were found in TW, IH, free glucose in the liver and muscle, and protein in the liver. The protein concentration in the muscle sharply increased at 24 and 360 h after feeding (60%). The results indicate a distinct and specific response of central components of carbohydrate, lipid, and protein metabolism that could be adjustments at the biochemical level upon periods of irregular feeding and even of long‐term food deprivation inside coastal lagoons or estuaries. The distinct responses of digestive enzymes in the intestine and energy reserves in the liver and muscle suggest the differential modulation of tissue‐specific anabolic and catabolic pathways that would allow the maintenance of physical conditions.

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A meta-analysis for assessing the contributions of trypsin and chymotrypsin as the two major endoproteases in protein hydrolysis in fish intestine

Cited by 11 publications

References 58 publications

Effect of Microplastics on the Activity of Digestive and Oxidative-Stress-Related Enzymes in Peled Whitefish (Coregonus peled Gmelin) Larvae

Effect of Microplastics on the Activity of Digestive and Oxidative-Stress-Related Enzymes in Peled Whitefish (Coregonus peled Gmelin) Larvae

Graded levels of dietary pink oyster mushroom, Pleurotus djamor meal, affects growth, feed efficiency, lipase activity and fiber content in final whole body of Nile tilapia fingerlings, Oreochromis niloticus

Differential modulation of digestive enzymes and energy reserves at different times after feeding in juveniles of the marine estuarine‐dependent flatfish Paralichthys orbignyanus (Valenciennes, 1839)

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