A mesh microelectrode array for non-invasive electrophysiology within neural organoids

McDonald, Matthew; D, Sebinger; Brauns, Lisa; Gonzalez-Cano, Laura; Menuchin-Lasowski, Yotam; Mierzejewski, Michael; Psathaki, Olympia-Ekaterini; Stumpf, Angelika; Wickham, Jenny; Rauen, Thomas; Schöler, Hans R.; Jones, Peter D.

doi:10.1016/j.bios.2023.115223

Cited by 18 publications

(20 citation statements)

References 37 publications

(38 reference statements)

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“…Flexible polyimide-based components were produced following established methods (McDonald et al , 2020; Steins et al , 2022). A first layer of polyimide (6 μm) was spin-coated on 100 mm silicon carrier wafers.…”

Section: Methodsmentioning

confidence: 99%

A flexible implant towards acute intrapancreatic electrophysiology

Pascual

Brauns

Domes

et al. 2023

Preprint

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Microelectrode arrays (MEAs) have proven to be a powerful tool to study electrophysiological processes over the last decades with most technology developed for investigation of the heart or brain. Other targets in the field of bioelectronic medicine are the peripheral nervous system and its innervation of various organs. Beyond the heart and nervous systems, the beta cells of the pancreatic islets of Langerhans generate action potentials during the production of insulin. In vitro experiments have demonstrated that their activity is a biomarker for blood glucose levels, suggesting that recording their activity in vivo could support patients suffering from diabetes mellitus with long-term automated read-out of blood glucose concentrations. Here, we present a flexible polymer-based implant having 64 low impedance microelectrodes designed to be implanted to a depth of 10 mm into the pancreas. As a first step, the implant will be used in acute experiments in pigs to explore the electrophysiological processes of the pancreas in vivo. Beyond use in the pancreas, our flexible implant and simple implantation method may also be used in other organs such as the brain.

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Section: Methodsmentioning

confidence: 99%

A flexible implant towards acute intrapancreatic electrophysiology

Pascual

Brauns

Domes

et al. 2023

Preprint

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“…Novel real-time and closed-loop electrophysiology systems and imaging experiments at multiple spatial and temporal scales are underway. Electrophysiology measurements through shell electrodes (Huang et al, 2022), microelectrode arrays (Kagan et al, 2022a), microelectrode meshes (McDonald et al, 2023), and neuropixels (Jun et al, 2017;Efros et al, 2018) are being explored.…”

Section: Communicating With the Organoid -Multi-scale Data Acquisitio...mentioning

confidence: 99%

Organoid intelligence (OI) – The ultimate functionality of a brain microphysiological system

Smirnova

Pantoja

Hartung

2023

ALTEX

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“…5 A key distinction in 3D cultures is whether the cells are self-organized by bottom-up mechanisms or are engineered with top-down mechanisms. Bottom-up neural systems include brain organoids [6][7][8] and neurospheres. 9 Top-down systems are usually dissociated neurons or other cells distributed in a scaffold.…”

Section: Introductionmentioning

confidence: 99%

“…21 Recent approaches include integration of microelectrodes in 3D arrays using complex assembly methods 22,23 or designing 2D arrays to be embedded within 3D cultures. 8,24 In fact, a promising approach is to develop new microelectrode arrays (MEA) with integrated microfluidic structures. 25 Direct fabrication of microfluidics on microelectrode arrays allows alignment with optical precision (∼1 μm), and can be scalable.…”

Section: Introductionmentioning

confidence: 99%