A MEMS-based measurement system for evaluating the force-length relationship of human induced pluripotent stem cell-derived cardiomyocytes adhered on a substrate

Matsudaira, Kenei; Takahashi, Hidetoshi; Hirayama-Shoji, Kayoko; Nguyên, Thành Vinh; Tsukagoshi, Takuya

doi:10.1088/1361-6439/ab093d

Cited by 12 publications

(19 citation statements)

References 35 publications

(39 reference statements)

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“…A differential pressure in the range of ±10 Pa was applied to the cantilever using a pressure generator. The fractional resistance change of the cantilever was measured using the setup reported in previous studies [24][25][26][27][28][29][30][31][32], which consists of the Wheatstone bridge circuit and an amplifying circuit whose output is recorded using a ScopeCorder (Yokogawa Test & Measurement Co., Tokyo, Japan, DL850). The calibration result in Figure 2c shows the linear relationship between the differential pressure and the fractional resistance change of the cantilever.…”

Section: Piezoresistive Cantilevermentioning

confidence: 99%

MEMS-Based Pulse Wave Sensor Utilizing a Piezoresistive Cantilever

Nguyen

Mizuki

Tsukagoshi

et al. 2020

Sensors

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This paper reports on a microelectromechanical systems (MEMS)-based sensor for pulse wave measurement. The sensor consists of an air chamber with a thin membrane and a 300-nm thick piezoresistive cantilever placed inside the chamber. When the membrane of the chamber is in contact with the skin above a vessel of a subject, the pulse wave of the subject causes the membrane to deform, leading to a change in the chamber pressure. This pressure change results in bending of the cantilever and change in the resistance of the cantilever, hence the pulse wave of the subject can be measured by monitoring the resistance of the cantilever. In this paper, we report the sensor design and fabrication, and demonstrate the measurement of the pulse wave using the fabricated sensor. Finally, measurement of the pulse wave velocity (PWV) is demonstrated by simultaneously measuring pulse waves at two points using the two fabricated sensor devices. Furthermore, the effect of breath holding on PWV is investigated. We showed that the proposed sensor can be used to continuously measure the PWV for each pulse, which indicates the possibility of using the sensor for continuous blood pressure measurement.

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Section: Piezoresistive Cantilevermentioning

confidence: 99%

MEMS-Based Pulse Wave Sensor Utilizing a Piezoresistive Cantilever

Nguyen

Mizuki

Tsukagoshi

et al. 2020

Sensors

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“…While maximal force is generated close to resting sarcomere length [ 43 ], it might be that force generation is not significantly affected after Z-disc ablations. Nevertheless, further direct methods [ 44 – 46 ] should be performed to quantify local force generation after laser-based manipulation on a single cell level. The significant reduction of sarcomere shortening in case of ablation of a single Z-disc might speculatively be related to faster endogenous repair.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes

et al. 2021

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Whereas it is evident that a well aligned and regular sarcomeric structure in cardiomyocytes is vital for heart function, considerably less is known about the contribution of individual elements to the mechanics of the entire cell. For instance, it is unclear whether altered Z-disc elements are the reason or the outcome of related cardiomyopathies. Therefore, it is crucial to gain more insight into this cellular organization. This study utilizes femtosecond laser-based nanosurgery to better understand sarcomeres and their repair upon damage. We investigated the influence of the extent and the location of the Z-disc damage. A single, three, five or ten Z-disc ablations were performed in neonatal rat cardiomyocytes. We employed image-based analysis using a self-written software together with different already published algorithms. We observed that cardiomyocyte survival associated with the damage extent, but not with the cell area or the total number of Z-discs per cell. The cell survival is independent of the damage position and can be compensated. However, the sarcomere alignment/orientation is changing over time after ablation. The contraction time is also independent of the extent of damage for the tested parameters. Additionally, we observed shortening rates between 6–7% of the initial sarcomere length in laser treated cardiomyocytes. This rate is an important indicator for force generation in myocytes. In conclusion, femtosecond laser-based nanosurgery together with image-based sarcomere tracking is a powerful tool to better understand the Z-disc complex and its force propagation function and role in cellular mechanisms.

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“…They also combined this piezoresistive cantilever with a movable plate to study the force‐length relationship of hiPSC‐CMs at the cellular scale. [ 145 ] Moreover, Lee's group proposed a piezoresistive sensor‐integrated PDMS cantilever, which consisted of a glass body with metal patterns, a PDMS cantilever with microgrooves, and integrated piezoresistive sensor (Figure 6C). [ 146 ] By monitoring the changes in electrical signal passing through the strain senor, the bending of the cantilever caused by the contractile force of cardiomyocytes can be measured effectively and effortlessly.…”

Section: Contractile Force Detectionmentioning

confidence: 99%

Advances in Multidimensional Cardiac Biosensing Technologies: From Electrophysiology to Mechanical Motion and Contractile Force

Wei

Zhuang

et al. 2020

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Cardiovascular disease (CVD) is the number one killer in the world. According to the World Health Organization (WHO) estimation, about 17.9 million people died from CVD each year, accounting for 31% of all deaths worldwide. [1] Among these deaths, about 7.4 million died from coronary heart disease, while 6.7 million from a stroke. According to China cardiovascular disease report in 2018, [2] around 290 million people suffer from CVD, including 13 million strokes, 11 million coronary heart disease, 5 million pulmonary heart disease, 4.5 million heart failure, 2.5 million rheumatic heart disease, 2 million congenital heart disease, and 270 million hypertension. Cardiovascular disease was also the leading cause of all death in rural and urban areas, with 45.01% in rural areas and 42.61% in urban areas. According to heart disease and stroke statistics from the American Heart Association (AHA) in 2018, [3] around 92.1 million American adults have a certain form of cardiovascular disease or suffer from stroke sequelae. CVD killed 836546 Americans in 2015. Coronary heart disease was the leading cause (43.8%) of death in CVD, followed by stroke (16.8%), heart failure (9.0%), hypertension (9.4%), arterial disease (3.1%) and other cardiovascular diseases (17.9%). According to the European cardiovascular disease statistics in 2017, [4] around 3.9 million people died from CVD each year, which accounts for 45% of all deaths in Europe. Ischemic heart disease (IHD) and stroke are the main forms of CVD in Europe. Among all deaths in Europe each year, IHD is the leading single cause of mortality, responsible for 19% and 20% among men and women, while stroke is the second most common single cause of death, accounting for 9% in men and 13% in women. Therefore, the prevention and treatment of CVD have aroused extensive public concern worldwide. Moreover, cardiac toxicity induced by drugs has been the main cause of the withdrawal from the market and drugs attrition during the last decades. [5,6] On the one hand, drug-induced cardiotoxicity will also threaten people's health, even life. For example, acute arrhythmia can lead to sudden death, while Cardiovascular disease is currently a leading killer to human, while druginduced cardiotoxicity remains the main cause of the withdrawal and attrition of drugs. Taking clinical correlation and throughput into account, cardiomyocyte is perfect as in vitro cardiac model for heart disease modeling, drug discovery, and cardiotoxicity assessment by accurately measuring the physiological multiparameters of cardiomyocytes. Remarkably, cardiomyocytes present both electrophysiological and biomechanical characteristics due to the unique excitation-contraction coupling, which plays a significant role in studying the cardiomyocytes. This review mainly focuses on the recent advances of biosensing technologies for the 2D and 3D cardiac models with three special properties: electrophysiology, mechanical motion, and contractile force. These high-performance multidimensional cardiac models are popular and ef...

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A MEMS-based measurement system for evaluating the force-length relationship of human induced pluripotent stem cell-derived cardiomyocytes adhered on a substrate

Cited by 12 publications

References 35 publications

MEMS-Based Pulse Wave Sensor Utilizing a Piezoresistive Cantilever

MEMS-Based Pulse Wave Sensor Utilizing a Piezoresistive Cantilever

Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes

Advances in Multidimensional Cardiac Biosensing Technologies: From Electrophysiology to Mechanical Motion and Contractile Force

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