A Membrane-integrated Microfluidic Device to Study Permeation of Nanoparticles through Straight Micropores toward Rational Design of Nanomedicines

Sasaki, Naoki; Tatanou, Mariko; Suzuki, Tomoko; Anraku, Yasutaka; Kishimura, Akihiro; Kataoka, Kazunori; Sato, Kae

doi:10.2116/analsci.32.1307

Cited by 10 publications

(9 citation statements)

References 31 publications

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“…A schematic illustration of the fabrication procedure of the microfluidic device is shown in Fig. 2 Microfluidic devices with porous membranes were fabricated as described previously (12) with some modifications of the procedures of microchannel pattern formation. (13) Briefly, bracketlike plates [ Fig.…”

Section: Microfabricationmentioning

confidence: 99%

“…Poly(dimethylsiloxane) (PDMS) substrates possessing recessed channel patterns were fabricated as described previously. (12) Through-holes for tubes were punched on the top substrate as shown in Fig. 2(a), using a biopsy punch (BPP-10F, Kai Industries, Gifu, Japan).…”

Section: Microfabricationmentioning

confidence: 99%

“…Afterward, the membrane was sandwiched between the substrates possessing PDMS mortar layers as described previously. (12) PTFE tubes (0.46 mm id, 0.92 mm od, Chukoh Chemical Industries, Tokyo, Japan) were glued to the through-holes at the end of the microchannels with the PDMS prepolymer.…”

Section: Microfabricationmentioning

confidence: 99%

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Photolithography-Free Skin-on-a-Chip for Parallel Permeation Assays

Sasaki¹,

Tsuchiya²,

Kobayashi³

2019

Sensors and Materials

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Dermatitis (skin inflammation) is becoming a serious health problem that lowers the quality of life. Microfluidic devices have been employed as an alternative to conventional in vitro skin models utilized to evaluate irritants, allergens, or drug candidates for dermatitis. However, the following should be achieved: high-throughput analysis by parallelization, simple, easy, and inexpensive device fabrication, and real-time microscopic imaging. In this paper, a photolithography-free skin-on-a-chip for parallel permeation assays is presented. In this study, a consumer laser cutter is employed to form masters for substrates possessing branched microchannel patterns. A porous membrane is sandwiched between the substrates to form 24 crossings of the microchannels on a single device. HaCaT, a human skin keratinocyte cell line, is cultured until confluence on the membrane in the microchannels. Parallel permeation assays are demonstrated using fluorescent-dye-labelled dextran as a tracer and potassium dichromate as an irritant. We think that our technique will expand the use of skin-on-a-chip technology among biologists and pharmacologists, and will open up a new field of biosensing, drug development, and so on.

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Section: Microfabricationmentioning

confidence: 99%

Section: Microfabricationmentioning

confidence: 99%

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Photolithography-Free Skin-on-a-Chip for Parallel Permeation Assays

Sasaki¹,

Tsuchiya²,

Kobayashi³

2019

Sensors and Materials

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“…A porous membrane with uniform straight pores was integrated in a microfluidic device to study permeation of nanoparticles. 38 Furthermore, the effects of pore sizes and pressure differences across the membrane on nanoparticle permeation were examined. The experimentally determined permeability coefficient of the 1.0-μm pore membrane against the 100-nm-diameter nanoparticles agreed well with the theoretical value.…”

Section: •3 Permeability Testmentioning

confidence: 99%

Recent Progress in the Development of Microfluidic Vascular Models

Sato

2018

ANAL. SCI.

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The blood vessel is part of the circulatory system, and systemic circulation provides the blood supply to all tissues. Arteries are pathways through which the blood is carried, and the capillaries have a key role in material exchange to maintain the tissue environment. Blood vessels have structures appropriate for their functions, and their sizes and cell types are different. In this review, we introduced recent studies of the microfluidic vascular models. The model structures are classified mainly as poly(dimethylsiloxane) and hydrogel microchannels and self-assembled networks. Basic phenomena and functions were realized in vascular models, including fluid shear stress, cell strain, interstitial flow, endothelial permeation, angiogenesis, and thrombosis. In some models, endothelial cells were co-cultured with smooth muscle cells, pericytes, and fibroblasts in an extracellular matrix. Examples of vascular models involving the brain, lung, liver, kidney, placenta, and cancer were also introduced.

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“…4) Ideally, this analytical method should also allow determination of the released (free) drug, as drug efficacy can only be measured following release of the encapsulated drug from the nanoparticle. 5) Hence, the determination of both the nanoparticle and the free drug is essential to allow evaluation of the quality, efficacy, and safety of such nanomedicines.…”

mentioning

confidence: 99%

Rapid Analysis of DOXIL Stability and Drug Release from DOXIL by HPLC Using a Glycidyl Methacrylate-Coated Monolithic Column

et al. 2017

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In recent years, nanomedicines have received growing attention in a range of medical applications, including selective drug delivery technology. In this context, the analysis of liposome stability and drug release from liposomes is of particular importance, as the efficacy of a nanomedicine is determined by the release of the encapsulated drug. We investigated the influence of the surrounding environment on the stability and release of the encapsulated drug (i.e., doxorubicin) from DOXIL. Thus, for the purpose of this study, we selected the liposomal anticancer drug, DOXIL, as a typical nanomedicine, and investigated the influence of the surrounding environment on release of doxorubicin from DOXIL. We found that two pathways existed for doxorubicin release, namely the collapse of DOXIL, and an increase in the permeability of the lipid bilayer. DOXIL collapse occurred upon the addition of high concentrations (>60%) of a methanol solution, while an increase in permeability occurred at temperatures above the phase transition temperature of the DOXIL lipid bilayer, under basic conditions, and in the presence of membrane-permeable bases (e.g., Tris). As DOXIL is particularly stable and limited collapse of DOXIL occurred under physiological conditions, it is expected that doxorubicin release within the body took place through permeability changes in the lipid bilayer of the DOXIL structure.

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A Membrane-integrated Microfluidic Device to Study Permeation of Nanoparticles through Straight Micropores toward Rational Design of Nanomedicines

Cited by 10 publications

References 31 publications

Photolithography-Free Skin-on-a-Chip for Parallel Permeation Assays

Photolithography-Free Skin-on-a-Chip for Parallel Permeation Assays

Recent Progress in the Development of Microfluidic Vascular Models

Rapid Analysis of DOXIL Stability and Drug Release from DOXIL by HPLC Using a Glycidyl Methacrylate-Coated Monolithic Column

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