A membrane‐bound NAC transcription factor NTL8 regulates gibberellic acid‐mediated salt signaling in Arabidopsis seed germination

Kim, Sang-Gyu; Lee, An-Kyo; Yoon, Hye-Kyung; Park, Chung‐Mo

doi:10.1111/j.1365-313x.2008.03493.x

Cited by 181 publications

(156 citation statements)

References 36 publications

(46 reference statements)

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“…Controlled proteolytic release of membrane-bound transcription factors is emerging as a versatile way of inducing rapid transcriptional changes in plants, e.g., in response to environmental changes (26). Recently, the functions of several membranebound NAC transcription factors were documented as well as their stimulus-dependent nuclear translocation (34)(35)(36)(37). The Cvi FSQ6/ANAC089 protein lacks the membrane anchor because of the premature stop mutation.…”

Section: Discussionmentioning

confidence: 99%

Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain

Wind

Shi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

115

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In living organisms sugars not only provide energy and carbon skeletons but also act as evolutionarily conserved signaling molecules. The three major soluble sugars in plants are sucrose, glucose, and fructose. Information on plant glucose and sucrose signaling is available, but to date no fructose-specific signaling pathway has been reported. In this study, sugar repression of seedling development was used to study fructose sensitivity in the Landsberg erecta (Ler)/Cape Verde Islands (Cvi) recombinant inbred line population, and eight fructose-sensing quantitative trait loci (QTLs) (FSQ1-8) were mapped. Among them, FSQ6 was confirmed to be a fructose-specific QTL by analyzing near-isogenic lines in which Cvi genomic fragments were introgressed in the Ler background. These results indicate the existence of a fructose-specific signaling pathway in Arabidopsis. Further analysis demonstrated that the FSQ6-associated fructose-signaling pathway functions independently of the hexokinase1 (HXK1) glucose sensor. Remarkably, fructosespecific FSQ6 downstream signaling interacts with abscisic acid (ABA)-and ethylene-signaling pathways, similar to HXK1-dependent glucose signaling. The Cvi allele of FSQ6 acts as a suppressor of fructose signaling. The FSQ6 gene was identified using mapbased cloning approach, and FSQ6 was shown to encode the transcription factor gene Arabidopsis NAC (petunia No apical meristem and Arabidopsis transcription activation factor 1, 2 and Cup-shaped cotyledon 2) domain containing protein 89 (ANAC089). The Cvi allele of FSQ6/ANAC089 is a gain-of-function allele caused by a premature stop in the third exon of the gene. The truncated Cvi FSQ6/ ANAC089 protein lacks a membrane association domain that is present in ANAC089 proteins from other Arabidopsis accessions. As a result, Cvi FSQ6/ANAC089 is constitutively active as a transcription factor in the nucleus. I n plants, sugars provide the energy and carbon skeletons needed for growth and in addition act as crucial signaling molecules that affect growth, development, and response to the (a)biotic environment (1-3). Plant cells harbor sugar-sensing and -signaling systems that regulate the expression of thousands of genes and control the metabolic processes needed for growth (2-4). These sugar-response systems are known to interact with other signaling pathways, such as those for light, phytohormones, stress, and nutrients (1).The neutral sugars sucrose, glucose, and fructose are central to metabolism in plants and in other organisms as well. So far, detailed information is available only on glucose sensing, and it has been shown that the hexokinase 1 (HXK1) enzyme acts as a glucose sensor (5, 6). Sucrose-specific signaling also was demonstrated because the effect of sucrose cannot be mimicked by glucose and/or fructose (7), but so far no information on sucrosesensing systems is available. A signaling function for fructose has been proposed (8, 9), but no convincing experimental evidence on such fructose-specific signaling is available.In Arabidopsis ea...

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Section: Discussionmentioning

confidence: 99%

Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain

Wind

Shi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

115

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“…A novel wheat NAC TF, TaNAC4 was found to be induced in response to cold, salt, wounding, ABA, ethylene and MeJA, suggesting a significant cross-talk between abiotic and biotic stress conditions (Xia et al, 2010). Kim et al (2008) reported that a salt-inducible NTL8 (membrane associated NAC) regulates gibberellic acid -mediated salt signaling in seed germination. Very recently a membrane associated NAC TF from foxtail millet was found to be upregulated in drought, salinity, ethephone and MeJA treatments (Puranik et al, 2011).…”

Section: The Nac (Nam Ataf and Cuc) And Zf-hd (Zinc-finger Homeodomamentioning

confidence: 99%

Role of Plant Transcription Factors in Abiotic Stress Tolerance

Lata¹,

Yadav²,

Prasad³

2011

Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives

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“…Fourteen of the NAC transcription factors in Arabidopsis are membranebound transcription factors with transmembrane domains (Kim et al, 2007a;Liang et al, 2015). Among them, NAC with Transmembrane Motif1 (NTM1) has been reported to participate in the regulation of cell division (Kim et al, 2006), NTM1-like6 (NTL6) is involved in the regulation of pathogen resistance response (Seo et al, 2010), NTL14/ANAC089 controls ER-stress-induced programmed cell death (Yang et al, 2014), NTL4 participates in heat-stress response (Lee et al, 2014), NTL1/ANAC013 is involved in oxidative stress response (De Clercq et al, 2013), and NTL8 regulates salt-responsive flowering and seed germination (Kim et al, 2007b(Kim et al, , 2008.…”

mentioning

confidence: 99%

NTL8 Regulates Trichome Formation in Arabidopsis by Directly Activating R3 MYB Genes TRY and TCL1

Tian

Guo

et al. 2017

Plant Physiol.

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The NAM, ATAF1/2, and CUC (NAC) are plant-specific transcription factors that regulate multiple aspects of plant growth and development and plant response to environmental stimuli. We report here the identification of NTM1-LIKE8 (NTL8), a membrane-associated NAC transcription factor, as a novel regulator of trichome formation in Arabidopsis (Arabidopsis thaliana). From an activation-tagged Arabidopsis population, we identified a dominant, gain-of-function mutant with glabrous inflorescence stem. By using plasmid rescue and RT-PCR analyses, we found that NTL8 was tagged; thus, the mutant was named ntl8-1 Dominant (ntl8-1D). Recapitulation experiment further confirmed that the phenotype observed in the ntl8-1D mutant was caused by elevated expression of NTL8. Quantitative RT-PCR results showed that the expression level of the single-repeat R3 MYB genes TRIPTYCHON (TRY) and TRICHOMELESS1 (TCL1) was elevated in the ntl8-1D mutant. Genetic analyses demonstrated that NTL8 acts upstream of TRY and TCL1 in the regulation of trichome formation. When recruited to the promoter region of the reporter gene Gal4:GUS by a fused GAL4 DNA-binding domain, NTL8 activated the expression of the reporter gene. Chromatin immunoprecipitation results indicated that TRY and TCL1 are direct targets of NTL8. However, NTL8 did not interact with SQUAMOSA PROMOTER BINDING PROTEIN LIKE9, another transcription factor that regulates the expression of TRY and TCL1, in yeast and plant cells. Taken together, our results suggest that NTL8 negatively regulates trichome formation in Arabidopsis by directly activating the expression of TRY and TCL1.

show abstract

A membrane‐bound NAC transcription factor NTL8 regulates gibberellic acid‐mediated salt signaling in Arabidopsis seed germination

Cited by 181 publications

References 36 publications

Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain

Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain

Role of Plant Transcription Factors in Abiotic Stress Tolerance

NTL8 Regulates Trichome Formation in Arabidopsis by Directly Activating R3 MYB Genes TRY and TCL1

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