A Membrane‐Associated Light‐Harvesting Model is Enabled by Functionalized Assemblies of Gene‐Doubled TMV Proteins

Dai, Jing; Wilhelm, Kiera B.; Bischoff, Amanda J.; Pereira, J.H.; Dedeo, Michel T.; García-Almedina, Derek M.; Adams, Paul D.; Groves, Jay T.; Francis, Matthew B.

doi:10.1002/smll.202207805

Cited by 2 publications

(2 citation statements)

References 69 publications

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“…In a recent report, we have demonstrated the ability to incorporate the TMV-based light-harvesting model into a supported lipid bilayer. 49 We have also recently used the TMV model to identify sources of disorder in biomimetic lightharvesting systems and their effect on long-range energy transfer. 29 The ability to couple two TMV assemblies asymmetrically adds another layer of utility to the TMV-based model system by allowing energy transfer between donor and acceptor complexes at controlled distances and orientations to be examined.…”

Section: Discussionmentioning

confidence: 99%

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Protein-Based Model for Energy Transfer between Photosynthetic Light-Harvesting Complexes Is Constructed Using a Direct Protein–Protein Conjugation Strategy

Bischoff

Hamerlynck

et al. 2023

J. Am. Chem. Soc.

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Photosynthetic organisms utilize dynamic and complex networks of pigments bound within light-harvesting complexes to transfer solar energy from antenna complexes to reaction centers. Understanding the principles underlying the efficiency of these energy transfer processes, and how they may be incorporated into artificial light-harvesting systems, is facilitated by the construction of easily tunable model systems. We describe a protein-based model to mimic directional energy transfer between light-harvesting complexes using a circular permutant of the tobacco mosaic virus coat protein (cpTMV), which self-assembles into a 34-monomer hollow disk. Two populations of cpTMV assemblies, one labeled with donor chromophores and another labeled with acceptor chromophores, were coupled using a direct protein−protein bioconjugation method. Using potassium ferricyanide as an oxidant, assemblies containing o-aminotyrosine were activated toward the addition of assemblies containing p-aminophenylalanine. Both of these noncanonical amino acids were introduced into the cpTMV monomers through amber codon suppression. This coupling strategy has the advantages of directly, irreversibly, and site-selectively coupling donor with acceptor protein assemblies and avoids cross-reactivity with native amino acids and undesired donor−donor or acceptor−acceptor combinations. The coupled donor−acceptor model was shown to transfer energy from an antenna disk containing donor chromophores to a downstream disk containing acceptor chromophores. This model ultimately provides a controllable and modifiable platform for understanding photosynthetic interassembly energy transfer and may lead to the design of more efficient functional light-harvesting materials.

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Section: Discussionmentioning

confidence: 99%

“…In a recent report, we have demonstrated the ability to incorporate the TMV-based light-harvesting model into a supported lipid bilayer . We have also recently used the TMV model to identify sources of disorder in biomimetic light-harvesting systems and their effect on long-range energy transfer .…”

Section: Discussionmentioning

confidence: 99%

Protein-Based Model for Energy Transfer between Photosynthetic Light-Harvesting Complexes Is Constructed Using a Direct Protein–Protein Conjugation Strategy

Bischoff

Hamerlynck

et al. 2023

J. Am. Chem. Soc.

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Protein‐Based Controllable Nanoarchitectonics for Desired Applications

Li,

Zhang,

et al. 2024

Adv Funct Materials

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Controllable protein nanoarchitectonics refers to the process of manipulating and controlling the assembly of proteins at the nanoscale to achieve domain‐limited and accurate spatial arrangement. In nature, many proteins undergo precise self‐assembly with other structural domains to engage in synergistic physiological activities. Protein nanomaterials prepared through protein nanosizing have received considerable attention due to their excellent biocompatibility, low toxicity, modifiability, and versatility. This review focuses on the fundamental strategies used for controllable protein nanoarchitectinics, which include computational design, self‐assembly induction, template introduction, complexation induction, chemical modification, and in vivo assembly. Precise controlling of the nanosizing process has enabled the creation of protein nanostructures with different dimensions, including 0D spherical oligomers, 1D nanowires, nanorings, and nanotubes, as well as 2D nanofilms, and 3D protein nanocages. The unique biological properties of proteins hold promise for diverse applications of these protein nanomaterials, including in biomedicine, the food industry, agriculture, biosensing, environmental protection, biocatalysis, and artificial light harvesting. Protein nanosizing is a powerful tool for developing biomaterials with advanced structures and functions.

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A Membrane‐Associated Light‐Harvesting Model is Enabled by Functionalized Assemblies of Gene‐Doubled TMV Proteins

Cited by 2 publications

References 69 publications

Protein-Based Model for Energy Transfer between Photosynthetic Light-Harvesting Complexes Is Constructed Using a Direct Protein–Protein Conjugation Strategy

Protein-Based Model for Energy Transfer between Photosynthetic Light-Harvesting Complexes Is Constructed Using a Direct Protein–Protein Conjugation Strategy

Protein‐Based Controllable Nanoarchitectonics for Desired Applications

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