A membrane antigen of rabbit bursal equivalent cells

Chou, C.‐T.; Cinader, B.; Dubiski, S.

doi:10.1016/0008-8749(77)90116-2

Cited by 23 publications

(6 citation statements)

References 11 publications

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“…Antibodies against membrane differen tiation antigens have been previously used to enumerate B and T cells in different or gans of the rabbit [5,8]. During the last few years it has become apparent that immuno fluorescent staining of human lymphocytes involves a considerable number of cells bearing receptors for the Fc portion of IgG (FcR).…”

Section: Discussionmentioning

confidence: 99%

“…RABELA antiserum: Heterologous goat anti serum directed against rabbit bursal equivalent cell antigen was prepared as described previously [5], Briefly, female goats were immunized with appen dix cells, sera were heated for 30 min at 56 °C and absorbed with rabbit erythrocytes, thymocytes and bone marrow cells.…”

Section: Methodsmentioning

confidence: 99%

“…In the following, we have examined the importance of various factors influencing the identification of rabbit B cells by fluo rescent staining and the correlation between values obtained when immunoglobulin and RABELA [5] were used as markers of these cells.…”

Section: Introductionmentioning

confidence: 99%

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Surface Immunoglobulin Receptors of Rabbit Lymphoid Cells

Szymańska¹,

Alcala²,

Dubiski³

et al. 1980

Int Arch Allergy Immunol

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Rabbit lymphoid cells were stained with fluorescein-labelled antiallotype antibodies. The double layer technique was found to be more sensitive than the direct staining. Rabbit B cells are stained only via their surface immunoglobulin (sIg) receptors and not via the receptors for the Fc portion of the IgG. Peritoneal exudate macrophages do not carry sIg receptors and are stained via their Fc receptors. Removal of protein aggregates from the system and use of reagents prepared from F(ab’)₂ immunoglobulin fragments prevent staining of the, macrophages through their Fc receptors. There was a good agreement between the percent of RABELA-positive and sIg-containing spleen cells. In appendix there were approximately 20% more RABELA-positive than sIg-positive cells.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Surface Immunoglobulin Receptors of Rabbit Lymphoid Cells

Szymańska¹,

Alcala²,

Dubiski³

et al. 1980

Int Arch Allergy Immunol

Self Cite

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“…The antisera and the antigens defined by them were described in previous publications [5,7,14,17]. In short, RTLA is characterized by an antiserum raised in goats by im munization with thymus cells; the serum is absorbed with bone-marrow cells.…”

Section: Enumeration O F Cellsmentioning

confidence: 99%

Mitotic Response of Rabbit Peripheral Blood Lymphocytes to a Factor in Normal Rabbit Serum: A Possible Genetic Polymorphism

Yonish-Rouach¹,

Dubiski²,

Cinader³

1982

Int Arch Allergy Immunol

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Normal rabbit sera contain a mitogen capable of stimulating rabbit peripheral blood lymphocytes in in vivo culture to incorporate radioactive thymidine. Cells from spleen, appendix or peritoneal exudate did not respond when cultured under similar conditions. The responding peripheral blood lymphocytes are adherent cells, but not macrophages. Presence of responding peripheral blood lymphocytes is a dominant trait. The response is regulated by a nonadherent, radiation-sensitive suppressor cell and by a nonadherent, relatively radiation-resistant helper cell.

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“…It is our aim to characterize B and T cells of the rabbit, so as to prepare the way for experiments which depend on identified functional lymphoid subpopulations. Few biochemical studies have been carried out with rabbit cells, primarily because well-defined markers by which B and T rabbit lymphocytes can be distinguished have become available only recently [7,13].…”

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confidence: 99%

Effect of Cyclic Nucleotides, Isoproterenol and Cholera Toxin on DNA Synthesis Triggered by Mitogens

1978

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The effect of cyclic nucleotides, isoproterenol and cholera toxin on phytohaemagglutinin (PHA), concanavalin A (Con A) and anti-allotype-induced rabbit lymphoid cell proliferation was examined. Cholera toxin in concentrations ranging from 10(-8) microgram to 1 microgram per culture inhibited DNA synthesis, triggered by PHA, Con A and nocardia water-soluble mitogen (NWSM). It had the opposite effect on stimulation with antibodies directed against allotypic specificities of the immunoglobulin light chains: over the entire range of tested concentrations, cholera toxin stimulated DNA synthesis triggered by antibodies to Ab4, Ab5, Ab9 and to Aa1 allotypic specificities. Relatively high concentrations of dibutyryl adenosine 3':5'-cyclic monophosphate (cAMP) and isoproterenol (10(-3) M) inhibited mitogen-stimulated thymidine incorporation; lower concentrations (10(-9) M) had an enhancing effect. A similar enhancing effect was observed when high (10(-3) M) concentrations of dibutyryl guanosine 3':5'-cyclic monophosphate (cGMP) were used.

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A membrane antigen of rabbit bursal equivalent cells

Cited by 23 publications

References 11 publications

Surface Immunoglobulin Receptors of Rabbit Lymphoid Cells

Surface Immunoglobulin Receptors of Rabbit Lymphoid Cells

Mitotic Response of Rabbit Peripheral Blood Lymphocytes to a Factor in Normal Rabbit Serum: A Possible Genetic Polymorphism

Effect of Cyclic Nucleotides, Isoproterenol and Cholera Toxin on DNA Synthesis Triggered by Mitogens

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