A member of the transforming growth factor-β receptor family from Echinococcus multilocularis is activated by human bone morphogenetic protein 2

Zavala-Góngora, Ricardo; Kroner, Antje; Bernthaler, Peter; Knaus, Petra; Brehm, Klaus

doi:10.1016/j.molbiopara.2005.12.011

Cited by 43 publications

(38 citation statements)

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“…An ORF encoding a member of the TGF-β receptor type Ib family was identified in O. viverrini . The ORF included a 28 amino acid insertion absent from other type I TGF-β receptors, except for TR1 from the hydatid tapeworms of the genus Echinococcus (also members of the phylum Platyhelminthes) [43]. However, these two insertions did not share sequence identity (Figure 6A).…”

Section: Resultsmentioning

confidence: 99%

Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini

et al. 2007

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Section: Resultsmentioning

confidence: 99%

Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini

et al. 2007

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“…Of these, the ALK5 receptor group is typically activated by the TGF-b/Activin subfamily of ligands, the ALK3 group exclusively by BMP family ligands and the ALK1 group by ligands of both subfamilies [52][53][54]. In E. multilocularis, we have previously characterized the type I receptor EmTR1, which is a member of the ALK1 group on the basis of its L45-loop structure [55]. Similar to the situation in EmIR, EmTR1 displays significant homologies to known TGF-b/BMP receptors in the tyrosine kinase and ligand-binding domains but contains extensive inserts in the tyrosine kinase domain, the biochemical function of which is unknown [55].…”

Section: Receptor Serine/threonine Kinase Signalling In Echinococcusmentioning

confidence: 99%

“…In E. multilocularis, we have previously characterized the type I receptor EmTR1, which is a member of the ALK1 group on the basis of its L45-loop structure [55]. Similar to the situation in EmIR, EmTR1 displays significant homologies to known TGF-b/BMP receptors in the tyrosine kinase and ligand-binding domains but contains extensive inserts in the tyrosine kinase domain, the biochemical function of which is unknown [55]. Encoded on the parasite's genome are another two receptors of the type I family, of which one presumably transmits TGF-b/Activin signals, whereas the other is, like EmTR1, a member of the ALK1 receptor family, and a single type II receptor [4].…”

Section: Receptor Serine/threonine Kinase Signalling In Echinococcusmentioning

confidence: 99%

“…Upon heterologous expression of EmTR1 in HEK 293 cells, together with a human type II receptor of the BMP receptor family, specific phosphorylation of EmSmadB (but not EmSmadA and EmSmadC) at the conserved pCXpC motif was obtained in presence of exogenously added human BMP2 [55,57]. We meanwhile also obtained evidence that this was not dependent on the human type II receptor component, since EmTR1 is also able to form a functional BMP2-responsive receptor complex with the Echinococcus type II receptor (Bernthaler et al, unpublished observations).…”

Section: Receptor Serine/threonine Kinase Signalling In Echinococcusmentioning

confidence: 99%

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The role of evolutionarily conserved signalling systems in Echinococcus multilocularis development and host–parasite interaction

Brehm

2010

Med Microbiol Immunol

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Alveolar echinococcosis, one of the most serious and life-threatening zoonoses in the world, is caused by the metacestode larval stage of the fox-tapeworm Echinococcus multilocularis. Mostly due to its accessibility to in vitro cultivation, this parasite has recently evolved into an experimental model system to study larval cestode development and associated host-parasite interaction mechanisms. Respective advances include the establishment of axenic in vitro cultivation systems for parasite larvae as well as culture systems by which the early development of metacestode vesicles from totipotent parasite stem cells can be reconstituted under controlled laboratory conditions. A series of evolutionarily conserved signalling molecules of the insulin, epidermal growth factor and transforming growth factor-beta pathways that are able to functionally interact with corresponding host cytokines have been described in E. multilocularis and most likely play a crucial role in parasite development within the liver of the intermediate host. Furthermore, a whole genome sequencing project has been initiated by which a comprehensive picture on E. multilocularis cell-cell communication systems will be available in due time, including information on parasite cytokines that are secreted towards host tissue and thus might affect the immune response. In this article, an overview of our current picture on Echinococcus signalling systems will be given, and the potential to exploit these pathways as targets for anti-parasitic chemotherapy will be discussed.

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“…Cestode orthologs of some developmentally related genes have been recently identified Zavala-Góngora et al 2006, 2008. However, the set of identified cestode developmental genes or proteins is still relatively small and insufficient to define whole developmental pathways and associate them with typical cestode developmental processes, such as proglottization or body segmentation.…”

Section: Introductionmentioning

confidence: 99%

Proteomic survey of the cestode Mesocestoides corti during the first 24 hours of strobilar development

et al. 2010

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Despite the fact that cestodes represent major etiological agents of both human and domestic animal diseases, little is known about the molecular aspects of cestode development. In this work, Mesocestoides corti, a model cestode species, was studied from the early development of its larval form (tetrathyridium) into adult worms (strobilation) using different proteomic approaches. The protein profiles of M. corti tetrathyridia induced or not induced to undergo strobilation were compared. Proteomic mapping by two-dimensional gel electrophoresis showed the resolution of 248 and 154 spots from tetrathyridia that were subjected or not subjected to strobilation induction, respectively, allowing for the detection of at least nine spots exclusive to each group. Spot analysis by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) or MALDI-TOF MS/MS identified four reference proteins (six spots). LC-MS/MS analyses of protein extracts identified 66 proteins, eight of which were found exclusively in non-induced tetrathyridia, while 13 were found exclusively in strobilation-induced tetrathyridia. Among the proteins exclusively identified in strobilation-induced worms, there was a predominance of proteins with functions relating to chaperone activity and protein synthesis and turnover. Quantitative differential expression analysis between M. corti tetrathyridia prior to and after strobilation induction revealed six proteins upregulated in strobilation-induced worms; these proteins were involved in metabolic pathways, cell proliferation, and cytoskeletal rearrangement. Overall, despite the absence of a sequenced M. corti genome, using sequences from other platyhelminthes, we were able to establish comprehensive protein profiles for tetrathyridia prior to and after strobilation induction and identify several proteins potentially involved in the early events leading to strobilation.

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A member of the transforming growth factor-β receptor family from Echinococcus multilocularis is activated by human bone morphogenetic protein 2

Cited by 43 publications

References 43 publications

Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini

Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini

The role of evolutionarily conserved signalling systems in Echinococcus multilocularis development and host–parasite interaction

Proteomic survey of the cestode Mesocestoides corti during the first 24 hours of strobilar development

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