A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNA<sup>Ile</sup>(GAU)

Romani, Isidora; Manavski, Nikolay; Morosetti, Arianna; Tadini, Luca; Maier, Swetlana; Kühn, Kristina; Ruwe, Hannes; Schmitz‐Linneweber, Christian; Wanner, Gerhard; Leister, Dario; Kleine, Tatjana

doi:10.1104/pp.15.00964

Cited by 52 publications

(105 citation statements)

References 91 publications

(144 reference statements)

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“…This finding thus points to a putative additional function for mTERF6 in seed protein storage. Taken together, the results corroborate the involvement of mTERF6 in aminoacylation of trnI.2 (Romani et al ), because genes expressed with MTERF6 show significant overlap with sets that are expressed together with genes for several aminoacyl‐tRNA synthetases.…”

Section: Resultssupporting

confidence: 84%

“…A set of 358 genes whose mRNA levels differed more than twofold and significantly from those in wild-type (WT) plants was identified (see Appendix S2). The amount of the MTERF6 transcript in the mutant background was reduced to 12% of the normal level, compared with 10% as determined by real-time PCR analysis (Romani et al 2015), which confirms the reliability of the microarray experiment. Furthermore, for several genes, the expression patterns obtained by Affymetrix GeneChip analysis were verified by real-time PCR analysis ( Fig.…”

Section: Transcriptome Changes In the Mterf6-1 Mutantsupporting

confidence: 69%

“…The prors1-2 (Pesaresi et al 2006), mterf6-1 (Romani et al 2015), gun1 and gun5 (Susek et al 1993) and oeGLK1 and oeGLK2 (35S:GLK1 and 35S:GLK2 in the glk1 glk2 background, respectively) (Waters et al 2008) lines have been described previously. Arabidopsis thaliana plants were routinely grown on soil under greenhouse conditions in a 16/8 h light/dark cycle and prors1-2 and mterf6-1 leaf material for RNA extraction was harvested at the same developmental stage.…”

Section: Growth Conditions Plant Materials and Inhibitor Treatmentsmentioning

confidence: 99%

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Definition of a core module for the nuclear retrograde response to altered organellar gene expression identifies GLK overexpressors as gun mutants

Leister¹,

Kleine²

2016

Physiologia Plantarum

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Retrograde signaling can be triggered by changes in organellar gene expression (OGE) induced by inhibitors such as lincomycin (LIN) or mutations that perturb OGE. Thus, an insufficiency of the organelle-targeted prolyl-tRNA synthetase PRORS1 in Arabidopsis thaliana activates retrograde signaling and reduces the expression of nuclear genes for photosynthetic proteins.Recently, we showed that mTERF6, a member of the so-called mitochondrial transcription termination factor (mTERF) family, is involved in the formation of chloroplast (cp) isoleucine-tRNA. To obtain further insights into its functions, co-expression analysis of MTERF6, PRORS1 and two other genes for organellar aminoacyl-tRNA synthetases was conducted. The results suggest a prominent role of mTERF6 in aminoacylation activity, light signaling and seed storage. Analysis of changes in whole-genome transcriptomes in the mterf6-1 mutant showed that levels of nuclear transcripts for cp OGE proteins were particularly affected. Comparison of the mterf6-1 transcriptome with that of prors1-2 showed that reduced aminoacylation of proline (prors1-2) and isoleucine (mterf6-1) tRNAs alters retrograde signaling in similar ways. Database analyses indicate that comparable gene expression changes are provoked by treatment with LIN, norflurazon or high light. A core OGE response module was defined by identifying genes that were differentially expressed under at least four of six conditions relevant to OGE signaling. Based on this module, overexpressors of the Golden2-like transcription factors GLK1 and GLK2 were identified as genomes uncoupled mutants.

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Section: Resultssupporting

confidence: 84%

Section: Transcriptome Changes In the Mterf6-1 Mutantsupporting

confidence: 69%

Section: Growth Conditions Plant Materials and Inhibitor Treatmentsmentioning

confidence: 99%

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Definition of a core module for the nuclear retrograde response to altered organellar gene expression identifies GLK overexpressors as gun mutants

Leister¹,

Kleine²

2016

Physiologia Plantarum

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“…Consistent with the stunted growth of the mterf6-2 and mterf6-5 individuals [20,22], the seedling establishment of both mutants was delayed compared with Col-0 (e.g., at 6 DAS in the MS control medium, 10%, 37%, and 99% of the mterf6-2, mterf6-5 and Col-0 seeds yielded seedlings with fully expanded green cotyledons, respectively (Figure 1b)). However, at 10 DAS, 93% and 100% of seedling establishments were achieved for the mterf6-5 and the wild type, respectively, whereas mterf6-2 reached a maximum value of 77% at 11 DAS (Figure 1b).…”

Section: Resultssupporting

confidence: 62%

The Characterization of Arabidopsis mterf6 Mutants Reveals a New Role for mTERF6 in Tolerance to Abiotic Stress

Robles

Navarro-Cartagena

Ferrández‐Ayela

et al. 2018

IJMS

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Exposure of plants to abiotic stresses, such as salinity, cold, heat, or drought, affects their growth and development, and can significantly reduce their productivity. Plants have developed adaptive strategies to deal with situations of abiotic stresses with guarantees of success, which have favoured the expansion and functional diversification of different gene families. The family of mitochondrial transcription termination factors (mTERFs), first identified in animals and more recently in plants, is likely a good example of this. In plants, mTERFs are located in chloroplasts and/or mitochondria, participate in the control of organellar gene expression (OGE), and, compared with animals, the mTERF family is expanded. Furthermore, the mutations in some of the hitherto characterised plant mTERFs result in altered responses to salt, high light, heat, or osmotic stress, which suggests a role for these genes in plant adaptation and tolerance to adverse environmental conditions. In this work, we investigated the effect of impaired mTERF6 function on the tolerance of Arabidopsis to salt, osmotic and moderate heat stresses, and on the response to the abscisic acid (ABA) hormone, required for plants to adapt to abiotic stresses. We found that the strong loss-of-function mterf6-2 and mterf6-5 mutants, mainly the former, were hypersensitive to NaCl, mannitol, and ABA during germination and seedling establishment. Additionally, mterf6-5 exhibited a higher sensitivity to moderate heat stress and a lower response to NaCl and ABA later in development. Our computational analysis revealed considerable changes in the mTERF6 transcript levels in plants exposed to different abiotic stresses. Together, our results pinpoint a function for Arabidopsis mTERF6 in the tolerance to adverse environmental conditions, and highlight the importance of plant mTERFs, and hence of OGE homeostasis, for proper acclimation to abiotic stress.

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“…The molecular characterization of maize Zm‐mTERF4 and Arabidopsis MTERF15 has revealed that they are required for the splicing of chloroplast and mitochondrial RNAs, respectively (Table ; Hammani and Barkan , Hsu et al ). Besides, it has been recently reported that MTERF6 promotes the maturation of chloroplast tRNA Ile , which suggests an additional function of MTERFs (Table ; Romani et al ). Hence, these results indicate that in plants, as in animals, MTERFs play a key role in OGE.…”

Section: The Mterf Family Of Proteinsmentioning

confidence: 95%

The roles of mitochondrial transcription termination factors (MTERFs) in plants

Quesada

2016

Physiologia Plantarum

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Stress such as salinity, cold, heat or drought affect plant growth and development, and frequently result in diminished productivity. Unlike animals, plants are sedentary organisms that must withstand and cope with environmental stresses. During evolution, plants have developed strategies to successfully adapt to or tolerate such stresses, which might have led to the expansion and functional diversification of gene families. Some new genes may have acquired functions that could differ from those of their animal homologues, e.g. in response to abiotic stress. The mitochondrial transcription termination factor (MTERF) family could be a good example of this. Originally identified and characterized in metazoans, MTERFs regulate transcription, translation and DNA replication in vertebrate mitochondria. Plant genomes harbor a considerably larger number of MTERFs than animals. Nonetheless, only eight plant MTERFs have been characterized, which encode chloroplast or mitochondrial proteins. Mutations in MTERFs alter the expression of organelle genes and impair chloroplast or mitochondria development. This information is transmitted to the nucleus, probably through retrograde signaling, because mterf plants often exhibit changes in nuclear gene expression. This study summarizes the recent findings, mainly from the analysis of mterf mutants, which support an emerging role for plant MTERFs in response to abiotic stress.

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A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNA^Ile(GAU)

Cited by 52 publications

References 91 publications

Definition of a core module for the nuclear retrograde response to altered organellar gene expression identifies GLK overexpressors as gun mutants

Definition of a core module for the nuclear retrograde response to altered organellar gene expression identifies GLK overexpressors as gun mutants

The Characterization of Arabidopsis mterf6 Mutants Reveals a New Role for mTERF6 in Tolerance to Abiotic Stress

The roles of mitochondrial transcription termination factors (MTERFs) in plants

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A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNAIle(GAU)

Cited by 52 publications

References 91 publications

Definition of a core module for the nuclear retrograde response to altered organellar gene expression identifies GLK overexpressors as gun mutants

Definition of a core module for the nuclear retrograde response to altered organellar gene expression identifies GLK overexpressors as gun mutants

The Characterization of Arabidopsis mterf6 Mutants Reveals a New Role for mTERF6 in Tolerance to Abiotic Stress

The roles of mitochondrial transcription termination factors (MTERFs) in plants

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A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNA^Ile(GAU)