A melt-electrowritten filter for capture and culture of circulating colon cancer cells

Jørgensen, Mathias Lindh; Müller, Christoph; Sikkersoq, Mille; Nadzieja, Marcin; Zhang, Zhongyang; Su, Yingchun; Just, Jesper; Spindler, Karen‐Lise Garm; Chen, Menglin

doi:10.1016/j.mtbio.2020.100052

Cited by 12 publications

(22 citation statements)

References 41 publications

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“…With lower conductivity, melt electrospinning generates a more stable jet than solution electrospinning, where solvent evaporation further introduces unstable factors. Recently, inspired by the threedimensional (3D) printing technique, a melt electrospinning writing (MEW) process was proposed [20][21][22] , by which accurate positioning of submicron fibers that are~2 orders of magnitude smaller than 3D printing has been realized. Depending on the collecting speed, these submicron fibers can be either collected as coils at low speed or be stretched into straight lines at high speed 23 .…”

Section: Introductionmentioning

confidence: 99%

A hierarchically ordered compacted coil scaffold for tissue regeneration

Zhang

Wan

et al. 2020

NPG Asia Mater

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Hierarchically ordered scaffold has a great impact on cell patterning and tissue engineering. The introduction of controllable coils into a scaffold offers an additional unique structural feature compared to conventional linear patterned scaffolds and can greatly increase interior complexity and versatility. In this work, 3D coil compacted scaffolds with hierarchically ordered patterns and tunable coil densities created using speed-programmed melt electrospinning writing (sMEW) successfully led to in vitro cell growth in patterns with tunable cell density. Subcutaneous implantation in mice showed great in vivo biocompatibility, as evidenced by no significant increase in tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) levels in mouse serum. In addition, a lumbar vertebra was successfully printed for mesenchymal stem cells to grow in the desired pattern. A long-range patterned matrix composed of programmable short-range compacted coils enabled the design of complex structures, e.g., for tailored implants, by readily depositing short-range coil-compacted secondary architectures along with customized primary design.

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Section: Introductionmentioning

confidence: 99%

A hierarchically ordered compacted coil scaffold for tissue regeneration

Zhang

Wan

et al. 2020

NPG Asia Mater

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“…As PCL is highlighted in several reviews on clinical translation of tissue engineering products, the transition from university-led research to application in the clinic is often negatively impacted by the need to repeat experiments with medical-grade polymers. In this instance, our understand- Measured as M w = 80 kDa [31] [ 11,16,12,20,[22][23][24]26, Sigma Aldrich Product number 440744 [46] M n = 80 × 10 3 [ 5,21,56,57] M n = 35 × 10 3 M w = 83 × 10 3 [ 13] M w = 45 kDa [58][59][60] M n = 45 × 10 3 [ 61] [ 5,13,21,[56][57][58][59][60][61] Capa 6400 M w ≈ 37 × 10 3 a) MFI = 40 b) [ 62,63] Capa 6430…”

Section: Why Pcl?mentioning

confidence: 99%

Polymers for Melt Electrowriting

Kade

Dalton

2020

Adv Healthcare Materials

149

155

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Melt electrowriting (MEW) is an emerging high‐resolution additive manufacturing technique based on the electrohydrodynamic processing of polymers. MEW is predominantly used to fabricate scaffolds for biomedical applications, where the microscale fiber positioning has substantial implications in its macroscopic mechanical properties. This review gives an update on the increasing number of polymers processed via MEW and different commercial sources of the gold standard poly(ε‐caprolactone) (PCL). A description of MEW‐processed polymers beyond PCL is introduced, including blends and coated fibers to provide specific advantages in biomedical applications. Furthermore, a perspective on printer designs and developments is highlighted, to keep expanding the variety of processable polymers for MEW.

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“…With this approach, high quality MES substrates could be made that have applicability for biomedical [ 32,33 ] and filtration applications. [ 34 ]…”

Section: Figurementioning

confidence: 99%

“…[ 36 ] As one example, Chen and colleagues recently combined solution electrospinning with MEW to create a filter that traps specific circulating tumor cells. [ 34 ] Using the system here, a similar purpose product can be made without requiring solvent use. Similarly, solution electrospun tubes that are melt electrowritten upon for vascular approaches [ 32 ] could be made solvent‐free without compromising the “barrier effect” that the small diameter electrospun fibers are intended to provide.…”

Section: Figurementioning

confidence: 99%

“…While current manufacturing throughput for MES is notably low in this study, the placement of the electrospun fibers on a specific spot on the collector is highly predictable and the head small enough to be employed within a hybrid manufacturing approach (shown with MEW as an example in this study) for biomaterial applications where defined barriers are of interest. [ 32–34 ]…”

Section: Figurementioning

confidence: 99%

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Melt Electrospinning of Nanofibers from Medical‐Grade Poly(ε‐Caprolactone) with a Modified Nozzle

Großhaus

Bakırcı

Berthel

et al. 2020

Small

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Melt electrospun fibers, in general, have larger diameters than normally achieved with solution electrospinning. This study uses a modified nozzle to direct‐write melt electrospun medical‐grade poly(ε‐caprolactone) onto a collector resulting in fibers with the smallest average diameter being 275 ± 86 nm under certain processing conditions. Within a flat‐tipped nozzle is a small acupuncture needle positioned so that reduces the flow rate to ≈0.1 µL h−1 and has the sharp tip protruding beyond the nozzle, into the Taylor cone. The investigations indicate that 1‐mm needle protrusion coupled with a heating temperature of 120 °C produce the most consistent, small diameter nanofibers. Using different protrusion distances for the acupuncture needle results in an unstable jet that deposited poor quality fibers that, in turn, affects the next adjacent path. The material quality is notably affected by the direct‐writing speed, which became unstable above 10 mm min−1. Coupled with a dual head printer, first melt electrospinning, then melt electrowriting could be performed in a single, automated process for the first time. Overall, the approach used here resulted in some of the smallest melt electrospun fibers reported to date and the smallest diameter fibers from a medical‐grade degradable polymer using a melt processing technology.

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A melt-electrowritten filter for capture and culture of circulating colon cancer cells

Cited by 12 publications

References 41 publications

A hierarchically ordered compacted coil scaffold for tissue regeneration

A hierarchically ordered compacted coil scaffold for tissue regeneration

Polymers for Melt Electrowriting

Melt Electrospinning of Nanofibers from Medical‐Grade Poly(ε‐Caprolactone) with a Modified Nozzle

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