A Medicago truncatula EF-Hand Family Gene, MtCaMP1, Is Involved in Drought and Salt Stress Tolerance

Wang, Tianzuo; Zhang, Jinli; Tian, Qiuying; Zhao, Mingfeng; Zhang, Wenhao

doi:10.1371/journal.pone.0058952

Cited by 32 publications

(20 citation statements)

References 45 publications

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“…Higher MDA content can induce cell membrane damage, which further reduces salt tolerance of plants [24], [54]–[57]. In the present study, most of the IbNFU1- overexpressing sweetpotato plants had significantly lower MDA content compared to wild-type plants, also indicating the marked improvement of their salt tolerance (Table 2).…”

Section: Discussionsupporting

confidence: 50%

An Ipomoea batatas Iron-Sulfur Cluster Scaffold Protein Gene, IbNFU1, Is Involved in Salt Tolerance

et al. 2014

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Iron-sulfur cluster biosynthesis involving the nitrogen fixation (Nif) proteins has been proposed as a general mechanism acting in various organisms. NifU-like protein may play an important role in protecting plants against abiotic and biotic stresses. An iron-sulfur cluster scaffold protein gene, IbNFU1, was isolated from a salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line LM79 in our previous study, but its role in sweetpotato stress tolerance was not investigated. In the present study, the IbNFU1 gene was introduced into a salt-sensitive sweetpotato cv. Lizixiang to characterize its function in salt tolerance. The IbNFU1-overexpressing sweetpotato plants exhibited significantly higher salt tolerance compared with the wild-type. Proline and reduced ascorbate content were significantly increased, whereas malonaldehyde (MDA) content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbNFU1 up-regulated pyrroline-5-carboxylate synthase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS) scavenging genes was found in the transgenic plants under salt stress. These findings suggest that IbNFU1gene is involved in sweetpotato salt tolerance and enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system.

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Section: Discussionsupporting

confidence: 50%

An Ipomoea batatas Iron-Sulfur Cluster Scaffold Protein Gene, IbNFU1, Is Involved in Salt Tolerance

et al. 2014

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“…MDA is widely recognized as a marker for lipid peroxidation (RoyChoudhury et al 2007). Higher MDA content can induce cell membrane damage, which further reduces salt tolerance of plants (Deng et al 2013;Wang et al 2013b;Liu et al 2014a, b). The present study found that the contents of H 2 O 2 and MDA in IbSIMT1-overexpressing plants were significantly lower than in WT under salt stress, indicating the marked improvement of their salt tolerance (Table 2; Fig.…”

Section: Discussionmentioning

confidence: 53%

IbSIMT1, a novel salt-induced methyltransferase gene from Ipomoea batatas, is involved in salt tolerance

Liu

Song

et al. 2014

Plant Cell Tiss Organ Cult

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S-adenosyl-methionine (SAM)-dependent methyltransferase (MTase) genes are a multigene family; however, only a few have been characterized at the functional level. In the present study, a novel salt-induced SAM-dependent MTase gene, named IbSIMT1, was isolated from salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line ND98. IbSIMT1 contains a DUF248 domain of unknown function and an MTase domain. Expression of IbSIMT1 was up-regulated in sweetpotato under salt stress and abscisic acid treatment. The IbSIMT1-overexpressing sweetpotato (cv. Shangshu 19) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H 2 O 2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbSIMT1 up-regulated the salt stress responsive genes, including pyrroline-5-carboxylate synthase, pyrroline-5-carboxylate reductase, SOD, psbA and phosphoribulokinase genes under salt stress.These findings suggest that the novel IbSIMT1 gene is involved in sweetpotato salt tolerance and enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and increasing reactive oxygen species scavenging capacity.

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“…For further study, qRT-PCR was used to explore the expression pattern of MtCAS31 in different tissues. Actin (Medtr3g095530) was employed as the reference [35,36]. The highest MtCAS31 expression was detected in the roots (45-fold compared to leaves and 50fold compared to stems) ( Figure 2A).…”

Section: Mtcas31 Is a Positive Regulator Of The Drought Response In Mmentioning

confidence: 99%

Dehydrin MtCAS31 promotes autophagic degradation under drought stress

Liu

Feng

et al. 2019

Autophagy

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Drought stress seriously affects crop yield, and the mechanism underlying plant resistance to drought stress via macroautophagy/autophagy is not clear. Here, we show that a dehydrin, Medicago truncatula MtCAS31 (cold acclimation-specific 31), a positive regulator of drought response, plays a key role in autophagic degradation. A GFP cleavage assay and treatment with an autophagy-specific inhibitor indicated that MtCAS31 participates in the autophagic degradation pathway and that overexpressing MtCAS31 promotes autophagy under drought stress. Furthermore, we discovered that MtCAS31 interacts with the autophagy-related protein ATG8a in the AIM-like motif YXXXI, supporting its function in autophagic degradation. In addition, we identified a cargo protein of MtCAS31, the aquaporin MtPIP2;7, by screening an M. truncatula cDNA library. We found that MtPIP2;7 functions as a negative regulator of drought response. Under drought stress, MtCAS31 facilitated the autophagic degradation of MtPIP2;7 and reduced root hydraulic conductivity, thus reducing water loss and improving drought tolerance. Taken together, our results reveal a novel function of dehydrins in promoting the autophagic degradation of proteins, which extends our knowledge of the function of dehydrins.

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A Medicago truncatula EF-Hand Family Gene, MtCaMP1, Is Involved in Drought and Salt Stress Tolerance

Cited by 32 publications

References 45 publications

An Ipomoea batatas Iron-Sulfur Cluster Scaffold Protein Gene, IbNFU1, Is Involved in Salt Tolerance

An Ipomoea batatas Iron-Sulfur Cluster Scaffold Protein Gene, IbNFU1, Is Involved in Salt Tolerance

IbSIMT1, a novel salt-induced methyltransferase gene from Ipomoea batatas, is involved in salt tolerance

Dehydrin MtCAS31 promotes autophagic degradation under drought stress

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