A mechanistic study on tumour spheroid formation in thermosensitive hydrogels: experiments and mathematical modelling

Cui, Xiaolin; Dini, S; Dai, Sheng; Bi, Jian; Binder, Benjamin J.; Green, J. E. F.; Zhang, Hu

doi:10.1039/c6ra11699j

Cited by 29 publications

(30 citation statements)

References 48 publications

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“…It has been demonstrated that this material is able to promote stem cell proliferation and clustering, 37,38 which leads to enhanced cell function and survival rate. 25 The porous structure of the nanogel can maximize mass transport of nutrients, oxygen, and secretion of regenerative factors from the encapsulated cells (Figure 1 and Supporting Information Figure S1).…”

Section: Discussionmentioning

confidence: 99%

Heart Repair Using Nanogel-Encapsulated Human Cardiac Stem Cells in Mice and Pigs with Myocardial Infarction

et al. 2017

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Stem cell transplantation is currently implemented clinically but is limited by low retention and engraftment of transplanted cells and the adverse effects of inflammation and immunoreaction when allogeneic or xenogeneic cells are used. Here, we demonstrate the safety and efficacy of encapsulating human cardiac stem cells (hCSCs) in thermosensitive poly(N-isopropylacrylamine-co-acrylic acid) or P(NIPAM-AA) nanogel in mouse and pig models of myocardial infarction (MI). Unlike xenogeneic hCSCs injected in saline, injection of nanogel-encapsulated hCSCs does not elicit systemic inflammation or local T cell infiltrations in immunocompetent mice. In mice and pigs with acute MI, injection of encapsulated hCSCs preserves cardiac function and reduces scar sizes, whereas injection of hCSCs in saline has an adverse effect on heart healing. In conclusion, thermosensitive nanogels can be used as a stem cell carrier: the porous and convoluted inner structure allows nutrient, oxygen, and secretion diffusion but can prevent the stem cells from being attacked by immune cells.

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Section: Discussionmentioning

confidence: 99%

Heart Repair Using Nanogel-Encapsulated Human Cardiac Stem Cells in Mice and Pigs with Myocardial Infarction

et al. 2017

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“…As a result, their aqueous suspensions can gel under appropriate conditions, forming a three‐dimensional (3D) network crosslinked physically with a hierarchical structure, a high surface area, and easily tunable mesh size. As a newly developed hydrogel material, the bulky gels formed from the thermoresponsive microgels have shown great potential in various applications such as 3D cell scaffolds, drug carriers, and blood vessel embolic material . Therefore, the thermally induced gelation behavior of thermoresponsive microgels has attracted much research attention in recent years …”

Section: Introductionmentioning

confidence: 99%

Rheological study on the thermoinduced gelation behavior of poly(N‐isopropylacrylamide‐co‐acrylic acid) microgel suspensions

Wang

Jin

et al. 2017

J of Applied Polymer Sci

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Submicron-sized thermoresponsive poly(N-isopropylacrylamide-co-acrylic acid) microgels were synthesized by soap-free emulsion polymerization. The physical state of the microgel suspensions, in a wide range of polymer concentrations (1.1-7.1 wt %), transformed from fluid to gel when the temperature was elevated across their volume phase transition temperature at pH 5 3.0. Such thermoinduced gelation behavior was studied in detail by small-deformation oscillatory rheological measurements within the linear viscoelastic region. It was found that the gelation temperature was strongly affected by the polymer concentration, decreasing as the polymer concentration increased. The gelation kinetics showed that the suspension gelled more quickly at either larger polymer concentration or higher isothermal heating temperature. In an isothermal frequency sweep for the as-formed gels, both storage and loss moduli, G 0 and G 00 , exhibited a power-law behavior, that is, G 0 (x) x 0.2-0.4 and G 00 (x) x 0.4620.50 within the frequency range where G 0 dominates G 00 . In addition, the elasticity of the gels, which results from the attractive particle bonds, increased markedly with increasing polymer concentration.

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“…cell [82] synthesized polymers PEG -good biocompatibility -irreversible gelation leading to challenges in releasing MCSs from gel Huh7.5 cells [12], submadibular glands [83] PLG/PLGA -biodegradable and biocompatible hepatocytes [84], MCF-7, U87 [49] NIPAM-based hydrogel -thermally reversible to release MSCs during harvest -low viscoelasticity -porous structure with great nutrient, oxygen and waste transport HepG2 [85], HeLa [51], human pluripotent stem cells [7] poly(1-carpolacton)…”

Section: Micro-mouldingmentioning

confidence: 99%

Advances in multicellular spheroids formation

Cui¹,

Hartanto²,

Zhang³

2017

J. R. Soc. Interface.

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394

336

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Three-dimensional multicellular spheroids (MCSs) have a complex architectural structure, dynamic cell -cell/cell -matrix interactions and bio-mimicking in vivo microenvironment. As a fundamental building block for tissue reconstruction, MCSs have emerged as a powerful tool to narrow down the gap between the in vitro and in vivo model. In this review paper, we discussed the structure and biology of MCSs and detailed fabricating methods. Among these methods, the approach in microfluidics with hydrogel support for MCS formation is promising because it allows essential cell -cell/cell -matrix interactions in a confined space.

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A mechanistic study on tumour spheroid formation in thermosensitive hydrogels: experiments and mathematical modelling

Abstract: Thermo-reversible microgels to culture and harvest uniform-sized tumour spheroids with a narrow size-distribution.

Cited by 29 publications

References 48 publications

Heart Repair Using Nanogel-Encapsulated Human Cardiac Stem Cells in Mice and Pigs with Myocardial Infarction

Heart Repair Using Nanogel-Encapsulated Human Cardiac Stem Cells in Mice and Pigs with Myocardial Infarction

Rheological study on the thermoinduced gelation behavior of poly(N‐isopropylacrylamide‐co‐acrylic acid) microgel suspensions

Advances in multicellular spheroids formation

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