A mechanistic approach to the in-vitro resistance modulating effects of fluoxetine against meticillin resistant Staphylococcus aureus strains

Neto, João Batista de Andrade; Josino, Maria Aparecida Alexandre; Silva, Cecília Rocha da; Campos, Rosana de Sousa; Nascimento, Francisca Bruna Stefany Aires do; Sampaio, Letícia Serpa; Sá, Lívia Gurgel do Amaral Valente; Carneiro, Igor de Sá; Barroso, Fátima Daiana Dias; Silva, Lisandra Juvêncio da; Mesquita, Jacó Ricarte Lima de; Cavalcanti, Bruno C.; Moraes, Manoel Odoríco de; Júnior, Hélio Vitoriano Nobre

doi:10.1016/j.micpath.2018.11.056

Cited by 33 publications

(13 citation statements)

References 27 publications

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“…The terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) method was used to determine DNA fragmentation according to [31]. Two hundred cells were counted per sample to determine the percentage of positive cells.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of the antifungal effect of chlorogenic acid against strains of Candida spp. resistant to fluconazole: apoptosis induction and in silico analysis of the possible mechanisms of action

Silva

Sá

Santos

et al. 2022

Journal of Medical Microbiology

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Introduction. Candida spp. are commensal fungal pathogens of humans, but when there is an imbalance in the microbiota, or weak host immunity, these yeasts can become pathogenic, generating high medical costs. Gap Statement. With the increase in resistance to conventional antifungals, the development of new therapeutic strategies is necessary. This study evaluated the in vitro antifungal activity of chlorogenic acid against fluconazole-resistant strains of Candida spp. Mechanism of action through flow cytometry and in silico analyses, as well as molecular docking assays with ALS3 and SAP5, important proteins in the pathogenesis of Candida albicans associated with the adhesion process and biofilm formation. Results. The chlorogenic acid showed in vitro antifungal activity against the strains tested, causing reduced cell viability, increased potential for mitochondrial depolarization and production of reactive oxygen species, DNA fragmentation and phosphatidylserine externalization, indicating an apoptotic process. Concerning the analysis through docking, the complexes formed between chlorogenic acid and the targets Thymidylate Kinase, CYP51, 1Yeast Cytochrome BC1 Complex e Exo-B-(1,3)-glucanase demonstrated more favourable binding energy. In addition, chlorogenic acid presented significant interactions with the ALS3 active site residues of C. albicans, important in the adhesion process and resistance to fluconazole. Regarding molecular docking with SAP5, no significant interactions were found between chlorogenic acid and the active site of the enzyme. Conclusion. We concluded that chlorogenic acid has potential use as an adjuvant in antifungal therapies, due to its anti-Candida activity and ability to interact with important drug targets.

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Section: Methodsmentioning

confidence: 99%

“…Intracellular sterases hydrolyse CM-H 2 DCFDA to a non-fluorescent compound (NFDC), which is rapidly oxidized to NFDC (2′,7′-dichlorofluorescein), a compound that produces high fluorescence. The fluorescence intensity of DCF is proportional to the production of ERO [31].…”

Section: Assessment Of the Production Of Reactive Oxygen Speciesmentioning

confidence: 99%

Evaluation of the antifungal effect of chlorogenic acid against strains of Candida spp. resistant to fluconazole: apoptosis induction and in silico analysis of the possible mechanisms of action

Silva

Sá

Santos

et al. 2022

Journal of Medical Microbiology

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“…De Andrade Neto et al studied the possible mechanism of action of fluoxetine against MRSA. In fact, they carried out cytometric analysis revealing that treatment with fluoxetine resulted in alterations in the integrity of plasma membranes and DNA damage, which provoked cell death, possibly by apoptosis [ 51 ].…”

Section: Antidepressants and Antipsychoticsmentioning

confidence: 99%

Non-Antibiotic Drug Repositioning as an Alternative Antimicrobial Approach

et al. 2022

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The worldwide scenario of antibiotic resistance and the falling number of funds for the development of novel antibiotics have led research efforts toward the study of specific cost-effective strategies aimed at discovering drugs against microbial infections. Among the potential options, drug repositioning, which has already exhibited satisfactory results in other medical fields, came out as the most promising. It consists of finding new uses for previously approved medicines and, over the years, many “repurposed drugs” displayed some encouraging in vitro and in vivo results beyond their initial application. The principal theoretical justification for reusing already existing drugs is that they have known mechanisms of action and manageable side effects. Reuse of old drugs is now considered an interesting approach to overcome the drawbacks of conventional antibiotics. The purpose of this review is to offer the reader a panoramic view of the updated studies concerning the repositioning process of different classes of non-antibiotic drugs in the antimicrobial field. Several research works reported the ability of some non-steroidal anti-inflammatory drugs (NSAIDs), antidepressants, antipsychotics, and statins to counteract the growth of harmful microorganisms, demonstrating an interesting winning mode to fight infectious diseases caused by antimicrobial resistant bacteria.

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“…Indeed, polyurethane linkages from the capsule's shell, in combination with intermolecular interactions between the drug and the nanocapsules hydrophilic core, allow for greater penetration into bacterial cells via layers of exopolysaccharides, resulting in an antimicrobial effect as evidenced by bacterial growth inhibition. [ 150,151 ]…”

Section: Other Starch Based Antimicrobial Polymersmentioning

confidence: 99%

Current State and Future Perspectives of Starch Derivatives and Their Blends as Antimicrobial Materials

Kanth

Puttaiahgowda

2022

Starch Stärke

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Antimicrobial resistance is increasingly recognized as a serious worldwide threat, jeopardizing medical progress, and the ability to treat infectious illness. Antimicrobial polymers have sparked the interest of researchers because of the increased demand for enhancing human health by lowering illness caused by different bacteria. Polysaccharides are ideal starting materials for the synthesis of antimicrobial polymers due to their bio renewability and broad availability. Starch is one such polysaccharide which has many advantages such as low-cost, nontoxicicity, and renewable sourcing and thus can be used for the synthesis of antimicrobial polymers. Here, the most recent research on starch-based antimicrobial polymers in the context of microbial infections is given in this article. The possibility of employing starch based films to alleviate environmental waste and prevent food spoiling is discussed in this paper. The goal of this study is to show the barrier and mechanical properties of starch containing antimicrobial agents, as well as their antimicrobial activity, in order to improve product storability. This study aims to advance knowledge in this area and urges additional research into the synthesis of starch-based antibacterial materials.

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A mechanistic approach to the in-vitro resistance modulating effects of fluoxetine against meticillin resistant Staphylococcus aureus strains

Cited by 33 publications

References 27 publications

Evaluation of the antifungal effect of chlorogenic acid against strains of Candida spp. resistant to fluconazole: apoptosis induction and in silico analysis of the possible mechanisms of action

Evaluation of the antifungal effect of chlorogenic acid against strains of Candida spp. resistant to fluconazole: apoptosis induction and in silico analysis of the possible mechanisms of action

Non-Antibiotic Drug Repositioning as an Alternative Antimicrobial Approach

Current State and Future Perspectives of Starch Derivatives and Their Blends as Antimicrobial Materials

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