A Mechanistic Analysis of Enzymatic Degradation of Organohalogen Compounds

Abstract

Abbreviations: HAD, haloacid dehalogenase; L-DEX YL, L-2-haloacid dehalogenase from Pseudomonas sp. YL; DL-DEX 113, DL-2-haloacid dehalogenase from Pseudomonas sp. 113; DehI, DL-2-haloacid dehalogenase from Pseudomonas putida strain PP3; FAc-DEX H1, fluoroacetate dehalogenase from Delftia acidovorans strain B; FAc-DEX FA1, fluoroacetate dehalogenase from Burkholderia sp. FA1; QM/MM, quantum mechanical/molecular mechanical; 2-CAA, 2-chloroacrylate; PAGE, polyacrylamide gel electrophoresis

Inverting hydrolases and their use in enantioconvergent biotransformations

“…Haloacid dehalogenases catalyze the hydrolytic dehalogenation of α-haloalkanoic acids to the corresponding α-hydroxyalkanoic acids (Figure 3) [58]. They have been classified either according to their sequence [59] or based on their stereoselectivity [60,61]; in this review the latter classification is used.…”

D-Specific Dehalogenases, a Review

“…enzymes involved in the conversion of organohalogen compounds have potential applications in environmental technologies and the chemical industry (18,23,24). For the catabolism of recalcitrant halogenated organic compounds in the biosphere, dehalogenation is regarded as the key first step (12).…”

Enhancement of haloacetate dehalogenase production by strain mutation and condition optimization

“…However, the microorganism was unable to grow in 3-CPS as a sole carbon source. Since the reported chloroacetate dehalogenase enzymes showed no activity with 3-CPS, and 3-chloropropionate dehalogenases have no activity with CAS [12,15,16], the chloroacetate dehalogenase presented in this study may be a novel enzyme.…”