IntroductionRâRas GTPase has recently been implicated in the regulation of immune functions, particularly in dendritic cell (DC) maturation, immune synapse formation, and subsequent T cell responses.MethodsHere, we investigated the role of RâRas in allergenâinduced immune response (type 2 immune response) in Rras deficient (RâRas KO) and wild type (WT) mice.ResultsInitially, we found that the number of conventional DC's in the lymph nodes (LNs) was reduced in RâRas KO mice. The expression of coâstimulatory CD80 and CD86 molecules on these cells was also reduced on DC's from the RâRas KO mice. However, there was no difference in papainâinduced immune response between the RâRas WT and KO as measured by serum IgE levels after the immunization. Interestingly, neither the DC number nor coâstimulatory molecule expression was different between WT and RâRas KO animals after the immunization.ConclusionsTaken together, despite having reduced number of conventional DC's in the RâRas KO mice and low expression of CD80 on DC's, the RâRas KO mice are capable of mounting papainâinduced IgE responses comparable to that of the WT mice. To our knowledge, this is the first report addressing potential differences in in vivo allergen responses regulated by the RâRas GTPase.