A mechanism for cryptic female choice in chinook salmon

Rosengrave, Patrice; Gemmell, Neil J.; Metcalf, Victoria J.; McBride, Katherine P.; Montgomerie, Robert

doi:10.1093/beheco/arn089

Cited by 108 publications

(162 citation statements)

References 60 publications

Supporting

Mentioning

145

Contrasting

Order By: Relevance

“…Beforehand, however, we confirmed that male-byfemale OF-sperm velocity interactions, reported previously in O. tshawytscha [23], were detectable in the experimental stock used for this study, and under the fertilization conditions imposed in our subsequent competitive fertilization assays. For these preliminary trials, we tested sperm velocity from six males crossed with OF from six females in all (n ¼ 36) combinations.…”

Section: (B) Experimental Overviewsupporting

confidence: 90%

“…This was necessary because in this study, OF from each focal female had to be partitioned across four fertilization trials within each block of the design (see below), requiring us to use OF at a lower concentration (OF : river H 2 O ¼ 1 : 10 ¼ 10% OF) in the competitive fertilization assays (see below) to that used previously (50% OF) to demonstrate OF -sperm interaction in O. tshawytscha [23]. In other respects, our methods for this preliminary experiment closely follow those described previously for O. tshawytscha [23]. Briefly, we conducted a factorial experiment involving sperm from six males and OF solutions from six females (OF diluted to 10% with raceway water).…”

Section: (C) Test For Ovarian Fluid -Sperm Interactionsmentioning

confidence: 99%

See 1 more Smart Citation

Delineating the roles of males and females in sperm competition

et al. 2013

Self Cite

View full text Add to dashboard Cite

Disentangling the relative roles of males, females and their interactive effects on competitive fertilization success remains a challenge in sperm competition. In this study, we apply a novel experimental framework to an ideally suited externally fertilizing model system in order to delineate these roles. We focus on the chinook salmon, Oncorhynchus tshawytscha, a species in which ovarian fluid (OF) has been implicated as a potential arbiter of cryptic female choice for genetically compatible mates. We evaluated this predicted sexually selected function of OF using a series of factorial competitive fertilization trials. Our design involved a series of 10 factorial crosses, each involving two 'focal' rival males whose sperm competed against those from a single 'standardized' (non-focal) rival for a genetically uniform set of eggs in the presence of OF from two focal females. This design enabled us to attribute variation in competitive fertilization success among focal males, females (OF) and their interacting effects, while controlling for variation attributable to differences in the sperm competitive ability of rival males, and male-by-female genotypic interactions. Using this experimental framework, we found that variation in sperm competitiveness could be attributed exclusively to differences in the sperm competitive ability of focal males, a conclusion supported by subsequent analyses revealing that variation in sperm swimming velocity predicts paternity success. Together, these findings provide evidence that variation in paternity success can be attributed to intrinsic differences in the sperm competitive ability of rival males, and reveal that sperm swimming velocity is a key target of sexual selection.

show abstract

Section: (B) Experimental Overviewsupporting

confidence: 90%

Section: (C) Test For Ovarian Fluid -Sperm Interactionsmentioning

confidence: 99%

Delineating the roles of males and females in sperm competition

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…Prior work in several fish species has shown that ovarian fluid, a viscous substance surrounding the spawned ova, enhances sperm longevity, per cent motility, path linearity and sperm velocity compared to water (for example, Litvak and Trippel, 1998;Turner and Montgomerie, 2002;Rosengrave et al, 2008;Galvano et al, 2013). The degree of stimulation of sperm velocity by the ovarian fluid is greatly explained by male-female interactions, which is indicative of the presence of CFC acting to promote gamete fusions of particular female-male combinations (Urbach et al, 2005;Rosengrave et al, 2008). Because sperm velocity is positively linked with fertilization success in fish (Gage et al, 2004;Boschetto et al, 2011;Evans et al, 2013;Rosengrave et al, 2016), it is possible that the differential stimulation of sperm velocity by ovarian fluid represents a mechanism of CFC (Rosengrave et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Sexual selection for genetic compatibility: the role of the major histocompatibility complex on cryptic female choice in Chinook salmon (Oncorhynchus tshawytscha)

et al. 2017

View full text Add to dashboard Cite

Cryptic female choice (CFC), a form of sexual selection during or post mating, describes processes of differential sperm utilization by females to bias fertilization outcomes towards certain males. In Chinook salmon (Oncorhynchus tshawytscha) the ovarian fluid surrounding the ova of a given female differently enhances the sperm velocity of males. Sperm velocity is a key ejaculate trait that determines fertilization success in externally fertilizing fishes, thus the differential effect on sperm velocity might bias male fertilization outcomes and represent a mechanism of CFC. Once sperm reach the oocyte, CFC could potentially be further facilitated by sperm-egg interactions, which are well understood in externally fertilizing marine invertebrates. Here, we explored the potential genetic basis of both possible mechanisms of CFC by examining whether the genotypic combinations of mates (amino-acid divergence, number of shared alleles) at the major histocompatibility complex (MHC) class I and II explain the variation in sperm velocity and/or male fertilization success that is not explained by sperm velocity, which might indicate MHC-based sperm-egg interactions. We recorded sperm velocity in ovarian fluid, employed paired-male fertilization trials and evaluated the fertilization success of each male using microsatellite-based paternity assignment. We showed that relative sperm velocity was positively correlated with fertilization success, confirming that the differential effect on sperm velocity may be a mechanism of CFC in Chinook salmon. The variation in sperm velocity was independent of MHC class I and II. However, the MHC class II divergence of mates explained fertilization success, indicating that this locus might influence sperm-egg interactions.

show abstract

“…Body size is the male trait directly preferred by females (Andersson & Simmons 2006); the mechanism suggested by which a cryptic female choice for male's genetic quality is exerted in externally fertilising fish is through ovarian fluid that facilitates sperm function in the Arctic charr (Turner and Montgomerie 2002) and in the Atlantic cod (Litvak and Trippel 1998). The effect of ovarian fluid on sperm performance depends on the physiological compatibility between male and female partners (Rosengrave et al 2008). Mate choice is believed to optimize variation on Major Histocompatibility Complex (MHC) genes that confer resistance to disease.…”

Section: Sperm Competition and Maternal Effectsmentioning

confidence: 99%

Ejaculate Allocation and Sperm Competition in Alternative Reproductive Tactics of Salmon and Trout: Implications for Aquaculture

Vladić¹

2011

Recent Advances in Fish Farms

View full text Add to dashboard Cite

A mechanism for cryptic female choice in chinook salmon

Cited by 108 publications

References 60 publications

Delineating the roles of males and females in sperm competition

Delineating the roles of males and females in sperm competition

Sexual selection for genetic compatibility: the role of the major histocompatibility complex on cryptic female choice in Chinook salmon (Oncorhynchus tshawytscha)

Ejaculate Allocation and Sperm Competition in Alternative Reproductive Tactics of Salmon and Trout: Implications for Aquaculture

Contact Info

Product

Resources

About