A Mechanism for Action of Oscillating Electric Fields on Cells

Panagopoulos, Dimitris J.; Messini, Niki; Karabarbounis, A.; Philippetis, A. L.; Margaritis, Lukas H.

doi:10.1006/bbrc.2000.2746

Cited by 117 publications

(166 citation statements)

References 28 publications

Supporting

Mentioning

149

Contrasting

Unclassified

Order By: Relevance

“…There are also hundreds of studies of oxidative stress responses to low intensity field exposures, which can also be produced by downstream effects of increased [Ca2+]i (1-3). The mode of microwave action via VGCC activation also confirms earlier predictions of Panagopoulos et al (7,8) that EMFs may act via voltage-gated ion channel activation. The whole issue of the biophysics of VGCCs and other voltagegated ion channels is discussed in some detail below.…”

Section: Emfs Act Via Stimulation Of Voltagegated Calcium Channels (Vsupporting

confidence: 85%

“…I believe it is likely that W.R. Adey was influenced by the plasma membrane properties when in the 1980s he proposed that a plasma membrane protein was the likely target of weak EMFs. Panagopoulos et al (7,8) may have been influenced by these plasma membrane and voltage sensor considerations when they decided to do biophysical modeling on voltage gated ion channels. The two reviewers of this paper each had some criticisms of the Panagopoulos et al (7,8) modeling, and some of the things in their papers go beyond my biophysics understanding, so I am unable to judge.…”

Section: The Properties Of Vgccs and Other Voltage-gated Ion Channelsmentioning

confidence: 99%

“…Panagopoulos et al (7,8) may have been influenced by these plasma membrane and voltage sensor considerations when they decided to do biophysical modeling on voltage gated ion channels. The two reviewers of this paper each had some criticisms of the Panagopoulos et al (7,8) modeling, and some of the things in their papers go beyond my biophysics understanding, so I am unable to judge. What I would say is that the modeling studies came to three important predictions: That voltagegated ion channels may be targets of low-intensity EMFs, that the VGCCs may be particularly activated because of the mechanism of the actual calcium flux through the channel and that pulsed fields may be more active than non-pulsed fields.…”

Section: The Properties Of Vgccs and Other Voltage-gated Ion Channelsmentioning

confidence: 99%

See 2 more Smart Citations

Scientific evidence contradicts findings and assumptions of Canadian Safety Panel 6: microwaves act through voltage-gated calcium channel activation to induce biological impacts at non-thermal levels, supporting a paradigm shift for microwave/lower frequency electromagnetic field action

Pall

2015

Reviews on Environmental Health

View full text Add to dashboard Cite

AbstractThis review considers a paradigm shift on microwave electromagnetic field (EMF) action from only thermal effects to action via voltage-gated calcium channel (VGCC) activation. Microwave/lower frequency EMFs were shown in two dozen studies to act via VGCC activation because all effects studied were blocked by calcium channel blockers. This mode of action was further supported by hundreds of studies showing microwave changes in calcium fluxes and intracellular calcium [Ca2+]i signaling. The biophysical properties of VGCCs/similar channels make them particularly sensitive to low intensity, non-thermal EMF exposures. Non-thermal studies have shown that in most cases pulsed fields are more active than are non-pulsed fields and that exposures within certain intensity windows have much large biological effects than do either lower or higher intensity exposures; these are both consistent with a VGCC role but inconsistent with only a heating/thermal role. Downstream effects of VGCC activation include calcium signaling, elevated nitric oxide (NO), NO signaling, peroxynitrite, free radical formation, and oxidative stress. Downstream effects explain repeatedly reported biological responses to non-thermal exposures: oxidative stress; single and double strand breaks in cellular DNA; cancer; male and female infertility; lowered melatonin/sleep disruption; cardiac changes including tachycardia, arrhythmia, and sudden cardiac death; diverse neuropsychiatric effects including depression; and therapeutic effects. Non-VGCC non-thermal mechanisms may occur, but none have been shown to have effects in mammals. Biologically relevant safety standards can be developed through studies of cell lines/cell cultures with high levels of different VGCCs, measuring their responses to different EMF exposures. The 2014 Canadian Report by a panel of experts only recognizes thermal effects regarding safety standards for non-ionizing radiation exposures. Its position is therefore contradicted by each of the observations above. The Report is assessed here in several ways including through Karl Popper’s assessment of strength of evidence. Popper argues that the strongest type of evidence is evidence that falsifies a theory; second strongest is a test of “risky prediction”; the weakest confirms a prediction that the theory could be correct but in no way rules out alternative theories. All of the evidence supporting the Report’s conclusion that only thermal effects need be considered are of the weakest type, confirming prediction but not ruling out alternatives. In contrast, there are thousands of studies apparently falsifying their position. The Report argues that there are no biophysically viable mechanisms for non-thermal effects (shown to be false, see above). It claims that there are many “inconsistencies” in the literature causing them to throw out large numbers of studies; however, the one area where it apparently documents this claim, that of genotoxicity, shows no inconsistencies; rather it shows that various cell types, fields and end points produce different responses, as should be expected. The Report claims that cataract formation is produced by thermal effects but ignores studies falsifying this claim and also studies showing [Ca2+]i and VGCC roles. It is time for a paradigm shift away from only thermal effects toward VGCC activation and consequent downstream effects.

show abstract

Section: Emfs Act Via Stimulation Of Voltagegated Calcium Channels (Vsupporting

confidence: 85%

Section: The Properties Of Vgccs and Other Voltage-gated Ion Channelsmentioning

confidence: 99%

Section: The Properties Of Vgccs and Other Voltage-gated Ion Channelsmentioning

confidence: 99%

See 1 more Smart Citation

Scientific evidence contradicts findings and assumptions of Canadian Safety Panel 6: microwaves act through voltage-gated calcium channel activation to induce biological impacts at non-thermal levels, supporting a paradigm shift for microwave/lower frequency electromagnetic field action

Pall

2015

Reviews on Environmental Health

View full text Add to dashboard Cite

show abstract

“…The present model, indeed inspired by the OHC, shares similar properties as a physical membrane embedded in viscoelastic surroundings that also functions as a mechanical amplifier at characteristic (resonant) frequencies [40,[63][64][65][66][67][68][69]. In this context, the dimensional power dissipation is given by the following expression:…”

Section: Dimensional Model A) Powermentioning

confidence: 99%

Linear oscillatory dynamics of flexoelectric membranes embedded in viscoelastic media with applications to outer hair cells

Abou-Dakka¹,

Herrera‐Valencia²,

Rey³

2012

Journal of Non-Newtonian Fluid Mechanics

115

View full text Add to dashboard Cite

Membrane flexoelectricity is an electromechanical coupling effect between the membrane average curvature and macroscopic electric polarization. Flexolelectricity is a biological actuation mechanism involved in the functioning of hearing. This thesis uses theory and simulation to develop a fundamental understanding of flexolectricity of relevance to hearing processes by integrating membrane elasticity and flexolectricity with viscoelastic processes.

show abstract

“…Electrical fields can affect multiple systems within cells, changing local membrane potential, position and conformation of proteins [Jaffe, 1977;Poo and Robinson, 1977] in the membranes, and ion flux across the plasma membrane [Panagopoulos et al, 2000;Thompson et al, 2000]. Previous work on the effect of electrical fields on wound healing has focused on the direct application of DC fields on the migration of single cells [Weninger et al, 2000] or monolayers [Cha et al, 1996] on simple matrices, providing data on the mechanisms involved in the stimulation and maintenance of migration.…”

Section: Introductionmentioning

confidence: 99%

The effect of induced biphasic pulsed currents on re‐epithelialization of a novel wound healing model

Bullock

Barker

Coulton

et al. 2006

Bioelectromagnetics

View full text Add to dashboard Cite

The coordinated migration of keratinocytes is crucial to cutaneous wound healing; failure of keratinocytes to migrate into a wound can lead to chronic non-healing wounds. Keratinocyte migration can be influenced by applied electrical fields. Our aim was to investigate whether keratinocyte migration could be accelerated by applying an induced biphasic pulsed electrical field. We developed two in vitro biological systems models for this purpose: a keratinocyte colony-forming model and a reconstituted skin wound healing model with biphasic pulsed currents. Our in vitro skin models were capable of generating trans-epithelial potentials (TEP) similar to in vivo mammalian skin. Histological examination of the wound healing model also indicated that re-epithelialization occurred in a similar manner to that seen in vivo, although no evidence of a reconstitution of a basement membrane was seen during the 14 days in vitro experimental period. We found that growth of keratinocyte colonies and keratinocyte migration in an in vitro wound bed were not significantly affected by induced short duration biphasic pulsed currents at a frequency of 0.5 Hz of 100 and 200 mV/mm.

show abstract

A Mechanism for Action of Oscillating Electric Fields on Cells

Cited by 117 publications

References 28 publications

Scientific evidence contradicts findings and assumptions of Canadian Safety Panel 6: microwaves act through voltage-gated calcium channel activation to induce biological impacts at non-thermal levels, supporting a paradigm shift for microwave/lower frequency electromagnetic field action

Scientific evidence contradicts findings and assumptions of Canadian Safety Panel 6: microwaves act through voltage-gated calcium channel activation to induce biological impacts at non-thermal levels, supporting a paradigm shift for microwave/lower frequency electromagnetic field action

Linear oscillatory dynamics of flexoelectric membranes embedded in viscoelastic media with applications to outer hair cells

The effect of induced biphasic pulsed currents on re‐epithelialization of a novel wound healing model

Contact Info

Product

Resources

About