Abstract:We have developed a measurement method for the cytochrome P450 3A4 (CYP3A4)mediated oxidation of cholesterol in lipid membranes. This method is based on the sterolstructural selectivity for the nanopore formation of streptolysin O (SLO) in a lipid membrane. A decrease in the fraction of 4β-hydroxycholesterol in lipid membranes decreased the nanopore forming ability of SLO. The incubation of the liposomes consisting of l-α-phosphatidylcholine (PC), l-α-phosphatidylethanolamine (PE), and cholesterol (0.98:0.02:0… Show more
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