A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken

Xia, Zemiao; Geng, Hui; Cai, Yuan; Wang, Yaonan; Sun, Dunlu; Zhang, Jian; Pan, Zhiming; Jiao, Xinan; Geng, Shizhong

doi:10.3389/fvets.2020.00324

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…The specific-pathogen free ( SPF ) Hyline White chickens were bought from Jinan Spafas Poultry CO., Ltd. (Jinan, Shandong, China). Three-day-old chicks were confirmed free from Salmonella infection by serum detection using O 9 Dc-ELISA ( Xia et al., 2020 ) and the bacteriological examination. All chickens were housed in separate rearing isolators by group and fed with a pathogen-free drinking water and commercial diet.…”

Section: Methodsmentioning

confidence: 99%

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

et al. 2022

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Salmonella enterica serovar Pullorum ( S. Pullorum) causes pullorum disease ( PD ), which is an acute systemic disease, in chickens, and leads to serious economic losses in many developing countries because of its high morbidity and mortality rate in young chicks. The live-attenuated vaccine is considered to be an effective measure to control the Salmonella infection. In addition, the DIVA (differentiation of infected and vaccinated animals) feature without the interference of serological monitoring of Salmonella infection is an important consideration in the development of the Salmonella vaccine. In this study, we evaluated the immunogenicity and protective efficacy of a S. Pullorum rough mutant S06004 ΔspiCΔrfaH as a live attenuated DIVA vaccine candidate in chickens. The S06004 ΔspiCΔrfaH exhibited a significant rough lipopolysaccharides ( LPS ) phenotype which was agglutinated with the acriflavine, not with the O 9 mono antibody. Compared to the wild-type, 50% lethal dose (LD 50 ) of the rough mutant increased 100-fold confirmed its attenuation. The mutant strain also showed a decreased bacterial colonization in the spleen and liver. The immunization with the mutant strain had no effect on the body weight and no tissue lesions were observed in the liver and spleen. The high level of the S. Pullorum-specific IgG titers in the serum indicated that significant humoral immune responses were induced in the immunization group. The cellular immune responses were also elicited from the analysis of lymphocyte proliferation and expression of cytokines in the spleen. In addition, the S06004 ΔspiCΔrfaH immunized group exhibited a negative response for the serological test, while the wild-type S06004 infection group was strongly positive for the serological test showing a DIVA capability. The survival rates in the vaccinated chickens were 87% after intramuscular challenge with wild-type S. Pullorum, while the survival rates were 20% in the control groups. Overall, these results have demonstrated that the rough mutant S06004 ΔspiCΔrfaH strain can be developed as an efficient live attenuated DIVA vaccine candidate to control the systemic S. Pullorum infection without the interference of salmonellosis monitoring program in poultry.

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Section: Methodsmentioning

confidence: 99%

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

et al. 2022

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“…The traditional methods for detecting pathogenic bacteria, such as microorganism culture and polymerase chain reaction, are constrained by their intricate operation, time-consuming nature, low sensitivity, specificity, etc. Recently, sensors based on various recognition elements (antibody, − aptamers, − antibiotics, ,, lectins, , and bacteriophages), have been extensively explored to detect pathogenic bacteria conveniently and specifically. However, some of these strategies suffer from drawbacks such as low sensitivity, complex operation, the need for large instruments, and high cost …”

Section: Introductionmentioning

confidence: 99%

Sensitive and on-Site Detection of Staphylococcus aureus Based on CRISPR/Cas 13a-Assisted Chemiluminescence Resonance Energy Transfer

Tao,

Yue,

Tian

et al. 2024

Anal. Chem.

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Developing a specific, sensitive, rapid, and on-site method for detecting pathogenic bacteria in food samples is critical to ensuring public safety. This article demonstrates a CRISPR/Cas13a system and a chemiluminescence resonance energy transfer (CRET) (CRISPR/Cas 13a-assisted CRET)-based strategy for sensitive and on-site detection of pathogenic bacteria in real samples. Once the hybrid double strand of aptamer S. aureus -cRNA recognizes the target model bacteria of Staphylococcus aureus (S. aureus), the released cRNA would bind with CRISPR/Cas 13a to form a complex of cRNA-CRISPR/Cas 13a, which could cleave the RNA molecule in the detecting probe of horseradish peroxidase (HRP) modified-gold nanoparticles (AuNPs) linked by RNA (AuNPs-RNA-HRP), resulting in an enhanced chemiluminescence signal due to the CRET “OFF” phenomenon after introducing the chemiluminescence substrate of luminol. The CRISPR/Cas 13a-assisted CRET strategy successfully detected S. aureus in drinking water and milk with detection limits of 20 and 30 cfu/mL, respectively, within the recovery of 90.07–105.50%. Furthermore, after integrating with an immunochromatographic test strip (ICTS), the CRISPR/Cas 13a-assisted CRET strategy achieved the on-site detection of as low as 102 cfu/mL of S. aureus in drinking water and milk via a smartphone, which is about 10 times lower than that in the previously reported AuNPs-based colorimetric ICTS, demonstrating a convenient and sensitive detection method for S. aureus in real samples.

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“…Serological detection of antibodies against lipopolysaccharides (LPS) of single or multiple serotypes has been used in national surveillance programs for poultry [ 3 , 8 ]. Other LPS-based enzyme-linked immunosorbent assays (ELISA) were developed later [ 27 ]. Because O-antigens in live or killed bacteria are equally recognised by the host immune system, this method cannot distinguish between an animal that is seropositive due to vaccination and that seropositive due to natural infection [ 24 ].…”

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confidence: 99%

Outer membrane protein BamA-based ELISA differentiates <i>Salmonella</i>-vaccinated chickens from naturally infected chickens

Aribam

Nakayama

Ogawa

et al. 2023

The Journal of Veterinary Medical Science

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Salmonella often causes subclinical infection in chickens, but antibody tests can find infected individuals and control the spread of infection. In this study, the S. Typhimurium-specific outer membrane, β-barrel assembly machinery protein A (BamA), was overexpressed in Escherichia coli and purified as a coating antigen to develop a BamA-based enzyme-linked immuno sorbent assay for detecting Salmonella infection. The presence of anti-BamA IgG was detected in the sera of infected BALB/c mice, but not in that of heat-killed Salmonella -vaccinated mice. The assay was validated using White Leghorn chickens and showed similar results. The detection of BamA antibodies in the sera can differentiate infected chickens from vaccinated chickens. This assay will be useful for monitoring Salmonella infection in chickens and possibly in other animals.

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A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken

Cited by 4 publications

References 21 publications

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

Sensitive and on-Site Detection of Staphylococcus aureus Based on CRISPR/Cas 13a-Assisted Chemiluminescence Resonance Energy Transfer

Outer membrane protein BamA-based ELISA differentiates <i>Salmonella</i>-vaccinated chickens from naturally infected chickens

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