A mating deficient and temperature-sensitive lethal mutant of Schizosaccharomyces pombe defines a new fertility locus

Rusu, M

doi:10.1007/bf00318648

Cited by 8 publications

(5 citation statements)

References 25 publications

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“…sep12 and sep14 are only partially sterile and thus cannot be assigned to either category. They are more similar to the 'grey' mutants defective in spi and mef genes (Sipiczki et al, 1984;Rusu, 1992).…”

Section: Discussionmentioning

confidence: 73%

Eleven novelsep genes ofSchizosaccharomyces pombe required for efficient cell separation and sexual differentiation

Grallert

Grallert²,

Zilahi

et al. 1999

Yeast

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Genetic analysis of 20 sterile mutants prone to form hyphae revealed 11 novel ste genes (sep6 to sep16) of Schizosaccharomyces pombe. None of the mutants was completely mycelial. Most mutants formed branching hyphae and showed normal septation. Aberrant septal structures and actin distribution were seen only at 36°C. sep9‐307, sep14‐576 and sep15‐598 showed genetic interactions with sep1‐1, a mutation in a forkhead transcription factor homologue. Additional genetic interactions were detected between sep6‐194, sep15‐598 and cdc16‐116, a mutant allele of an anaphase modulator of p34cdc2. sep9‐307 and sep15‐598 caused dikaryosis in wee1− background. In mating and sporulation tests, sep6−, sep7−, sep9−, sep10−, sep11− and sep15− proved to be defective in conjugation only, whereas sep8−, sep13− and sep16− were also defective in meiosis‐sporulation. sep12− and sep14− were only partially sterile. All mutants could produce M‐factor but sep8−, sep11−, sep15− and sep16− were defective in P‐factor production. The mutations in sep8, sep11 and sep16 suppressed the pat1‐114‐driven meiosis. All mutants were sensitive to the presence of higher concentrations of chloride in the medium and to short heat shocks. The diversity of the mutant phenotypes and the pleiotropic effects of the mutations suggest that these sep genes might act in, or interact with, a multiple overlapping network of regulatory modules. Copyright © 1999 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 73%

Eleven novelsep genes ofSchizosaccharomyces pombe required for efficient cell separation and sexual differentiation

Grallert

Grallert²,

Zilahi

et al. 1999

Yeast

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“…Such desensitization, or adaptation, also appears to be important in the mating process as mutations that reduce the ability to recover from stimulations also lead to reduced fertility (Watson and Davey, unpublished). 68,88,112,236 There are likely to be several mechanisms that contribute to the adaptation process and most of the components activated by pheromone stimulation will need to be deactivated during the recovery process.…”

Section: Adaptationmentioning

confidence: 99%

Fusion of a fission yeast

Davey

1998

Yeast

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“…Based on the observation of similar phenotypes (increased mating) of a strain carrying a recessive mutation in the anr2 locus and a strain carrying a deletion of rad24 (Rusu, 1992; Ozoe et al , 2002), we determined the acid phosphatase acitivity of the rad24 ‐deleted strain. Late exponential‐phase cultures of a rad24Δ strain in YEL supplemented with adenine exhibited about 18‐fold more acid phosphatase activity than the reference rad24 + (501‐1) strain.…”

Section: Resultsmentioning

confidence: 99%

“… a This mutant was first described as a suppressor of a mating‐deficient mutant (Rusu, 1992) and named smd1‐35 . By genetic tests it was later found to be allelic to anr2 mutants isolated by Schweigruber et al (1992). …”

Section: Methodsmentioning

confidence: 99%

In Schizosaccharomyces pombe the 14–3–3 protein Rad24p is involved in negative control of pho1 gene expression

Voicu

Petrescu-Danila

Poitelea

et al. 2006

Yeast

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Expression of Schizosaccharomyces pombe pho1-encoded acid phosphatase is transcriptionally regulated by adenine and phosphate. Four genes, anr1-3 and anr5, encode negative regulators of pho1 expression. Apart from being designated as loci, the anr genes have not been further characterized. In this study we provide evidence that a strain carrying the deletion of rad24, a 14-3-3 protein-encoding gene, exhibits an anr mutant like the phenotype (higher phosphatase activity, higher transcript levels of pho1, lower sensitivity to adenine of pho1 expression) and that rad24 is closely linked, probably allelic, to anr5. By sequencing the two exons of the rad24 gene in a strain carrying the mutant allele anr5-13, we found a T/A-to-C/G transition in the 225th codon of its ORF, causing a leucine-to-serine substitution in a highly conserved region of all proteins of the 14-3-3 family. anr2 and anr3 are not allelic to rad24. The mutant alleles of anr2 and anr3 are recessive to their wild-type alleles and do not belong to the same epistasis group as rad24.

show abstract

A mating deficient and temperature-sensitive lethal mutant of Schizosaccharomyces pombe defines a new fertility locus

Cited by 8 publications

References 25 publications

Eleven novelsep genes ofSchizosaccharomyces pombe required for efficient cell separation and sexual differentiation

Eleven novelsep genes ofSchizosaccharomyces pombe required for efficient cell separation and sexual differentiation

Fusion of a fission yeast

In Schizosaccharomyces pombe the 14–3–3 protein Rad24p is involved in negative control of pho1 gene expression

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