We address the existence of periodic orbits for periodic eco-epidemiological system with disease in the prey. To do it, we consider three main steps. Firstly we study a one parameter family of systems and obtain uniform bounds for the components of any periodic solution of these systems. Next, we make a suitable change of variables in our family of systems to establish the setting where we are able to apply Mawhin's continuation Theorem. Finally, we use Mawhin's continuation Theorem to obtain our result. Later on, we present two examples that include previous results in the literature and some numerical simulations to illustrate our results.Date: March 13, 2020. Key words and phrases. Eco-epidemiological system; periodic orbit; persistence. L. de Jesus, C. M. Silva and H. Vilarinho were partially supported by FCT through CMA-UBI (project UIDB/MAT/00212/2020).