A mathematical model for the study of lipid accumulation in oleaginous microorganisms. II. Study of cellular lipids of &lt;i&gt;Mucor circinelloides&lt;/i&gt; during growth on a vegetable oil

Aggelis, George; Komaitis, Michael; Papanikolaou, Séraphim; Papadopoulos, George

doi:10.3989/gya.1995.v46.i4-5.932

Cited by 27 publications

(19 citation statements)

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“…Fatty acid specificity of Yarrowia lipolytica can be quantified by calculating its fatty acid incorporation constant as proposed by Aggelis et al [6]. Specifically, it was demonstrated that substrate fat (S) was exponentially removed from the medium according to, [s] = A·e -k(S)·t , where k (h -1 ) is the substrate incorporation constant and related to the initial reaction conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Since the various fatty acid substrates are removed at different rates from the medium and incorporated into the cell, the fatty acid composition of the fat medium can be altered as a function of fermentation time [6][7][8]. Incorporated fatty acids are either degraded for growth needs or used as substrate for intracellular biotransformations.…”

Section: Introductionmentioning

confidence: 99%

“…Compositional modifications conducted in both fat medium and accumulated lipid, may lead to the production of "new" lipids with industrial and financial importance (e.g. storage lipids mainly composed of TAGs either rich in polyunsaturated fatty acids or with structures similar to exotic fats) [6,9,10], especially if various crude fats or fatty waste-waters of low value (presently generated in high quantities [11][12][13][14][15]), could be used as substrate. Yarrowia (Candida) lipolytica can be considered to be a model microorganism with the ability to modify fatty materials.…”

Section: Introductionmentioning

confidence: 99%

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Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Papanikolaou

Aggelis

2003

Euro J Lipid Sci & Tech

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Yarrowia lipolytica ACA-DC 50109, maintained on potato dextrose agar (PDA, Fluka, Hannover, Germany) at T = 4 °C, was used. The salt composition of the culture medium was (g/l): KH 2 PO 4 7, Na 2 HPO 4 2. Selective uptake of fatty acids by the yeast Yarrowia lipolyticaYarrowia lipolytica, when cultivated on mixtures of free fatty acid substrates was found to remove C12:0, C14:0, ∆9 C18:1, ∆9,12 C18:2 and ∆9,12,15 C18:3 at significantly higher rates than C16:0 and C18:0, regardless of fatty acid composition of the initial substrate. C12:0, C14:0 and ∆9,12,15 C18:3 were specifically and completely removed from the substrate, while ∆9 C18:1 and ∆9,12 C18:2 concentrations decreased by 55-80 wt-% in comparison with initial concentrations. In contrast, concentration of C18:0 increased 2.1-3.5 fold in the substrate. Although C18:0 was removed slowly from the substrate, this fatty acid was selectively accumulated in the storage lipid. Inversely, only low quantities of ∆9 C18:1 and ∆9,12 C18:2 and traces of C12:0, C14:0 and ∆9,12,15 C18:3 were accumulated in the storage lipid. During storage lipid breakdown, cellular C16:0 and ∆9 C18:1 were taken up more rapidly than C18:0. We concluded that the capability of Yarrowia lipolytica to selectively remove several fatty acids from the substrate and accumulate others could be used in modification of the composition of selected mixtures of fatty acids and probably of common fats, to produce "new" fats with a predetermined composition.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Papanikolaou

Aggelis

2003

Euro J Lipid Sci & Tech

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“…Storage lipid accumulation occurs during primary metabolic growth when fatty materials are used as substrate and therefore after the exhaustion of the substrate fat the culture environment is still favorable for growth [96,97,105]. For this reason, in all cases in which kinetic studies have been performed on fatty substrates lipid accumulated was always re-consumed in favor of lipid-free material generation regardless of the fatty acid composition of the fat used as substrate [39,89,[104][105][106][107][108].…”

Section: Ex Novo Lipid Accumulationmentioning

confidence: 99%

Lipids of oleaginous yeasts. Part II: Technology and potential applications

Papanikolaou

Aggelis

2011

Euro J Lipid Sci & Tech

343

279

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The process of lipid accumulation in the oleaginous yeasts cultivated in various fermentation configurations when either sugars and related compounds or hydrophobic substances are used as substrates is presented and kinetic models describing both de novo and ex novo lipid accumulation are analyzed. Technological aspects related with single cell oil (SCO) produced by oleaginous yeasts are depicted. The influence of culture parameters upon lipid production process is presented. Lipid production has been studied in batch, fed-batch, and continuous cultivation systems using yeasts belonging to the species Lipomyces starkeyi, Rhodosporidium toruloides, Apiotrichum curvatum, Candida curvata, Cryptococcus curvatus, Trichosporon fermentans, and Yarrowia lipolytica. The potentiality of yeasts to produce SCO as starting material of 2nd generation biodiesel is indicated and discussed. Of significant importance is also the utilization of yeast lipids as substitutes of high added value exotic fats (e.g., cocoa butter). Lipid produced by the various yeasts presents, in general, similar composition with that of common vegetable oils being composed of unsaturated fatty acids, whereas cocoa butter is principally composed of saturated fatty acids, consequently the various strategies that are followed in order to increase the cellular saturated fatty acid content of the yeast lipid are presented and comprehensively discussed.

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“…De novo synthesis of lipids in microbial cells decreases when the C W N ratio in the medium increases. 15) More unsaturated fatty acids are incorporated than saturated fatty acids in the microorganisms Mucor circinlloides CBS 172-27, 16) Mucor hiemalis HA-30, 5) and Candida lipolytica. 17) Therefore, we expected that the DHA accumulating in P. methanolica HA-32 would depress de novo synthesis of saturated and monounsaturated fatty acids in the cells, as would cultivation in a medium with a high nitrogen concentration.…”

Section: Discussionmentioning

confidence: 99%