A Maternal Effect on Queen Production in Honeybees

Wei, Hao; He, Xingjian; Liao, Chun Hua; Wu, Xiao; Jiang, Wu; Zhang, Bo; Zhou, Lin; Zhang, Li Zhen; Barron, Andrew B.; Zeng, Zhi

doi:10.1016/j.cub.2019.05.059

Cited by 28 publications

(59 citation statements)

References 47 publications

(71 reference statements)

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“…Although the queen is thought to be protected by several social immune mechanisms in the colony, she is exposed to different environmental stresses during different life stages that possibly have negative effects on her health and quality [24]. Initially, a queen can be influenced by the environment that her parents experienced as a result of a trans-generational stress response [25][26][27][28]. She can also be influenced by different environmental stressors during development, either through exposure to biotic or abiotic stressors in the colony environment or specifically via the quality or quantity of royal jelly she receives [29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Honey Bee Queens and Virus Infections

Amiri

Strand

Rueppell

2020

Viruses

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The honey bee queen is the central hub of a colony to produce eggs and release pheromones to maintain social cohesion. Among many environmental stresses, viruses are a major concern to compromise the queen’s health and reproductive vigor. Viruses have evolved numerous strategies to infect queens either via vertical transmission from the queens’ parents or horizontally through the worker and drones with which she is in contact during development, while mating, and in the reproductive period in the colony. Over 30 viruses have been discovered from honey bees but only few studies exist on the pathogenicity and direct impact of viruses on the queen’s phenotype. An apparent lack of virus symptoms and practical problems are partly to blame for the lack of studies, and we hope to stimulate new research and methodological approaches. To illustrate the problems, we describe a study on sublethal effects of Israeli Acute Paralysis Virus (IAPV) that led to inconclusive results. We conclude by discussing the most crucial methodological considerations and novel approaches for studying the interactions between honey bee viruses and their interactions with queen health.

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Section: Introductionmentioning

confidence: 99%

Honey Bee Queens and Virus Infections

Amiri

Strand

Rueppell

2020

Viruses

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“…6; Table S7, S8 and S11). The mTOR, Wnt, Notch, transforming growth factor-beta (TGF-β) and hippo signaling pathways play an essential role in the developmental processes, such as caste differentiation, embryogenesis, morphogenesis, imaginal disc development and organ size regulation in honeybees and other insects [24][25][26][27][28][29][30]. These results demonstrated a clear difference in gene expression between honeybee male larvae developed from drone cells and female cells.…”

Section: Discussionmentioning

confidence: 89%

“…Fold Change ≥ 1 and p < 0.05 were used as the screening criteria to identify signi cant DEGs among three groups. We selected 61 and 50 interesting genes that are involved in caste differentiation and development regulation [9,[25][26][27][28][29][30] from comparisons at larval and adult stages respectively. The log10 fold change values of these genes were used for heatmap analysis in R package [40]…”

Section: Analysis Of Pearson`s Correlationmentioning

confidence: 99%

Female Developmental Environment Delays Development of Male Honeybee (Apis Mellifera)

Liu

Yao

Abdelmawla

et al. 2020

Preprint

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Background Nutrition and cell size play an important role in the determination of caste differentiation in queen-worker of honeybee (Apis mellifera), whereas the haploid genome dominates the differentiation of drones. However, the effects of female developmental environment on the development of males remain unclear. In this study, young drone larvae were transferred into worker cells (WCs) or remained in drone cells (DCs) to rear drones. The parts of drone larvae were also grafted into queen cells (QCs) for 48 h and then transplanted into drone cells until emerging. Morphological indexes and reproductive organs of these three types of newly emerged drones were measured. Newly emerged drones and 3 d drone larvae from WCs, DCs and QCs were sequenced by RNA sequencing (RNA-Seq). Results Morphological results showed that newly emerged DC drones had bigger body sizes and more well-developed reproductive tissues than WC and QC drones, whereas the reproductive tissues of QC drones were relatively better than those of WC drones. Gene expression results showed a more clear difference among three groups. At the larval stage, there were 889, 1761 and 1927 significantly differentially expressed genes (DEGs) in WC/DC, QC/DC and WC/QC comparisons, respectively. The number of DEGs decreased in adult drones of these three comparisons [678 (WC/DC), 338 (QC/DC) and 518 (WC/QC)]. A high number of DEGs were involved in sex regulation, growth, olfaction, vision, mammalian target of rapamycin (mTOR), Wnt signaling pathways, etc. Weighted gene co-expression network analysis (WCGNA) showed that WC and DC larvae were closer than QC larvae, whereas QC and WC drones were closer than DC drones. These results revealed that DC drones had better development in the body and reproductive system than QC and WC drones. Conclusion This study demonstrated that the developmental environment of honeybee females including the larval diet and cell size delayed male development. Naturally, honeybee colony ovigerous workers in queen-less colonies or non-mated queens produce a large number of dysplasia drones which are not well-developed. Therefore, this study serves as a model for understanding the regulation of sexual differentiation in social insects by environmental factors.

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“…In total 9 pooled samples were collected. RNA libraries were prepared and sequenced using Illumina Hiseq 4000 following standard protocol by Gene Denovo Biotechnology Co. (Guangzhou, China) (Wei et al 2019).…”

Section: Rna Sequencing and Analysismentioning

confidence: 99%

“…However, the honeybee colonies have been declining in recent years (Neumann and Carreck 2010). Multiple factors have been associated with colony losses, including the parasitic mite Varroa destructor (Rosenkranz et al 2010), the fungal parasites Nosema ceranae (Antunez et al 2009), viruses (McMenamin and Genersch 2015), malnutrition, environmental degradation, climate change, queen quality decline (Wei et al 2019) and widespread insecticide application (Budge et al 2015;Sanchez-Bayo et al 2016).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Sub-lethal dose of Insecticides on Honeybee Behavior and Transcriptome

Zhang¹,

Li²,

Huang³

et al. 2021

Preprint

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Exposure to insecticides is the main cause of honeybee (Apis mellifera) population decline. At sub-lethal doses, these chemicals have been shown to negatively affect honeybee physiological development and behavior. Previously, we found the insecticide imidacloprid and deltamethrin significant reduced honeybee dancing and foraging efficiency. As a follow up, we performed a deep RNA-seq analysis to reveal the gene regulatory mechanisms underlying the altered foraging behavior. Genes involved in detoxification were up-regulated in both imidacloprid and deltamethrin treatment groups. Gene members in immune pathways, odorant receptors and major royal jelly protein families showed significant up or down regulation in treatment groups compared with controls. This fluctuating gene expression profile reflects that multifaceted aspects of honeybee physiology were affected by the two insecticides, leading to inaccurate communication and impaired learning and memory. Our findings reveal candidate molecular mechanisms under impaired dance performance in honeybees exposed to insecticides.

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A Maternal Effect on Queen Production in Honeybees

Abstract: Highlights d Honeybee queens lay larger eggs in queen cells than in worker cells d Egg size influences both gene expression and adult queen weight d The in ovo environment is a maternal effect influencing caste development in bees

Cited by 28 publications

References 47 publications

Honey Bee Queens and Virus Infections

Honey Bee Queens and Virus Infections

Female Developmental Environment Delays Development of Male Honeybee (Apis Mellifera)

The Effects of Sub-lethal dose of Insecticides on Honeybee Behavior and Transcriptome

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