A Master Key to Assess Stroke Consequences Across Species: The Adhesive Removal Test

Bouët, Valentine; Fréret, Thomas

doi:10.5772/32327

Cited by 8 publications

(8 citation statements)

References 57 publications

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“…In the adhesive removal tests sensorimotor functions and sensory asymmetries can be measured [ 45 ]. In the control group the average time to remove each piece of tape was 15.2 ± 1.3 s ( Figure 5 d).…”

Section: Resultsmentioning

confidence: 99%

“…The delicate manipulations of the forelimbs important for the normal life of rodents were analyzed using the sunflower seed task, vermicelli handling test, and the adhesive test, indicative of sensory and motor abilities of the forelimbs, tongue, and jaw [ 42 , 43 , 44 , 45 ]. In hHcy rats impaired manipulation of small objects (seeds, vermicelli) and slowing of the manipulation rate (adhesive test and seed task) was observed which may result from dysfunctions of cortex and subcortical areas responsible for the fine motor control and sensory feedback as also observed in Parkinson disease, cerebral ischemia, or craniocerebral injury [ 44 , 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

“…Bilateral tactile stimulation or adhesive removal test was developed to assess movement/sensory asymmetries due to unilateral nigrostriatal damage or stroke-related impairments [ 45 ]. The rat was placed in a clear plexiglass box and allowed to habituate for 1 min.…”

Section: Methodsmentioning

confidence: 99%

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Hydrogen Sulfide Alleviates Anxiety, Motor, and Cognitive Dysfunctions in Rats with Maternal Hyperhomocysteinemia via Mitigation of Oxidative Stress

et al. 2020

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Hydrogen sulfide (H2S) is endogenously produced from sulfur containing amino acids, including homocysteine and exerts neuroprotective effects. An increase of homocysteine during pregnancy impairs fetal growth and development of the offspring due to severe oxidative stress. We analyzed the effects of the H2S donor—sodium hydrosulfide (NaHS) administered to female rats with hyperhomocysteinemia (hHcy) on behavioral impairments and levels of oxidative stress of their offspring. Rats born from females fed with control or high methionine diet, with or without H2S donor injections were investigated. Rats with maternal hHcy exhibit increased levels of total locomotor activity and anxiety, decreased muscle endurance and motor coordination, abnormalities of fine motor control, as well as reduced spatial memory and learning. Oxidative stress in brain tissues measured by activity of glutathione peroxidases and the level of malondialdehyde was higher in rats with maternal hHcy. Concentrations of H2S and the activity and expression of the H2S generating enzyme—cystathionine-beta synthase—were lower compared to the control group. Administration of the H2S donor to females with hHcy during pregnancy prevented behavioral alterations and oxidative stress of their offspring. The acquisition of behavioral together with biochemical studies will add to our knowledge about homocysteine neurotoxicity and proposes H2S as a potential agent for therapy of hHcy associated disorders.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Hydrogen Sulfide Alleviates Anxiety, Motor, and Cognitive Dysfunctions in Rats with Maternal Hyperhomocysteinemia via Mitigation of Oxidative Stress

et al. 2020

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“…The adhesive removal test is very relevant in linking experimental and clinical stroke, as it was originally based on the double simultaneous stimulation examination of contralateral neglect [ 74 , 75 ]. Furthermore, it has been used across multiple species, namely mice, rats, gerbils and marmosets [ 20 , 23 , 24 , 76 , 77 ].…”

Section: Discussionmentioning

confidence: 99%

Evolution of ischemic damage and behavioural deficit over 6 months after MCAo in the rat: Selecting the optimal outcomes and statistical power for multi-centre preclinical trials

et al. 2017

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Key disparities between the timing and methods of assessment in animal stroke studies and clinical trial may be part of the reason for the failure to translate promising findings. This study investigates the development of ischemic damage after thread occlusion MCAo in the rat, using histological and behavioural outcomes. Using the adhesive removal test we investigate the longevity of behavioural deficit after ischemic stroke in rats, and examine the practicality of using such measures as the primary outcome for future studies. Ischemic stroke was induced in 132 Spontaneously Hypertensive Rats which were assessed for behavioural and histological deficits at 1, 3, 7, 14, 21, 28 days, 12 and 24 weeks (n>11 per timepoint). The basic behavioural score confirmed induction of stroke, with deficits specific to stroke animals. Within 7 days, these deficits resolved in 50% of animals. The adhesive removal test revealed contralateral neglect for up to 6 months following stroke. Sample size calculations to facilitate the use of this test as the primary experimental outcome resulted in cohort sizes much larger than are the norm for experimental studies. Histological damage progressed from a necrotic infarct to a hypercellular area that cleared to leave a fluid filled cavity. Whilst absolute volume of damage changed over time, when corrected for changes in hemispheric volume, an equivalent area of damage was lost at all timepoints. Using behavioural measures at chronic timepoints presents significant challenges to the basic science community in terms of the large number of animals required and the practicalities associated with this. Multicentre preclinical randomised controlled trials as advocated by the MultiPART consortium may be the only practical way to deal with this issue.

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“…Some such tests include composite scores of general welfare such as the Benderson Neurological Scoring Scales, the Modified Neurological Severity Scores (Bederson et al 1986; Zhang et al 2002), and tests for cognitive deficits such as the Modified Morris Water Maze and the Radial Arm Maze (Volpe et al 1984; Schallert 2006). Furthermore, a battery of tests have been developed to measure sensorimotor deficits, some of which are specific for forelimb use, such as: the Cylinder Test, the Reaching Pellet Retrieval Task, the Staircase Test, the Vibrissae Elicited Forelimb Placing Test, the Corner Test, the Accelerated Rotarod Test and the Adhesive Removal Test (Schallert et al 2000, 1982; Montoya et al 1991; Markgraf et al 1992; Baird et al 2001; Chen et al 2001; Gharbawie et al 2004; Schallert 2006; Allred and Jones 2008; Allred et al 2008; Bouet and Freret 2012); and others that are specific for hindlimb use, such as: the Grid Walking Test, the Ledged Tapered Beam Test and the Ladder Rung Walking Test (Metz and Whishaw 2002; Schallert and Woodlee 2005; Allred and Jones 2008). While it is apparent that there are various tests measuring deficits in forelimb use, there are far fewer tests that measure hindlimb functionality.…”

Section: Introductionmentioning

confidence: 99%

So you think you can jump? A novel long jump assessment to detect deficits in stroked mice

Mittal

Pan

Palmateer

et al. 2015

Journal of Neuroscience Methods

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Background Stroke survivors suffer from persistent disability, as well as severe sensorimotor and cognitive deficits. The preclinical assessment of such deficits is important for the development of novel interventions and therapeutics. New Method The aim of this study was to develop a quantitative behavioral measure of hindlimb functionality in rodents, which could be used to assess deficits after a neural injury, such as stroke. Here we introduce a test to measure long jump behavior in mice. Results Using this test we first showed that while male and female mice exhibited no differences in jump success rate, the female mice showed lower baseline jumping latencies. Next we demonstrated that the induction of a cerebral stroke via middle cerebral artery occlusion (MCAO) for 45 minutes did not affect the jump success rate in either group; however, it did significantly increase jump latencies in both male and female mice. Finally, we used therapeutic interventions to explore mechanisms that may be involved in producing this increase in jump latency by administering the anti-depressant fluoxetine prior to the long jump assay, and also tested for potential changes in anxiety levels after stroke. Comparison with Existing Methods Other methods to assess hindlimb functionality are not specific, because they measure behaviors that rely not only on hindlimbs, but also on forelimbs and tail. Conclusions This study introduces a novel assay that can be used to measure a stroke induced behavioral deficit with great sensitivity, and raises interesting questions about potential mechanisms regulating this effect.

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A Master Key to Assess Stroke Consequences Across Species: The Adhesive Removal Test

Cited by 8 publications

References 57 publications

Hydrogen Sulfide Alleviates Anxiety, Motor, and Cognitive Dysfunctions in Rats with Maternal Hyperhomocysteinemia via Mitigation of Oxidative Stress

Hydrogen Sulfide Alleviates Anxiety, Motor, and Cognitive Dysfunctions in Rats with Maternal Hyperhomocysteinemia via Mitigation of Oxidative Stress

Evolution of ischemic damage and behavioural deficit over 6 months after MCAo in the rat: Selecting the optimal outcomes and statistical power for multi-centre preclinical trials

So you think you can jump? A novel long jump assessment to detect deficits in stroked mice

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