A Marine Fibrinolytic Compound FGFC1 Stimulating Enzymatic Kinetic Parameters of a Reciprocal Activation System Based on a Single Chain Urokinase-Type Plasminogen Activator and Plasminogen

Abstract

A marine fibrinolytic compound FGFC1 enhancing fibrinolysis was obtained involving enzymatic kinetic parameters of a reciprocal activation system with a single chain urokinase type plasminogen activator and plasminogen. FGFC1, a kind of bisindole alkaloid from a metabolite of the rare marine fungi Starchbotrys longispora FG216, modulated enzymatic kinetic parameters including the fibrinolytic reaction rate and fibrin degradation characteristics. The enzymatic kinetics of fibrinolysis was described based on the enzymatic reaction of a chromogenic-substrate associated with p-nitroaniline (p-NA). While the single chain urokinase-type plasminogen activator (pro-uPA) activated plasminogen, kcat and kcat/Km increased significantly with an increase of FGFC1 concentration. Moreover, kcat and kcat/Km exhibited 26.5-fold and 22.8-fold enhanced activity at the concentration of 40 μg·mL −1 of FGFC1, respectively. The results suggested that FGFC1 significantly improved the maximum catalytic efficiency and the total catalytic activity of fibrinolysis base on the reciprocal activation of pro-uPA and plasminogen. Km increased with increasing FGFC1 concentration, which indicated that FGFC1 slightly decreased the affinity activity of the pro-uPA and plasminogen versus the enzyme substrate. The marine bisindole alkaloid FGFC1 enhanced fibrinolysis, which was taken on enzymatic kinetic characteristics.