“…Patients with symptoms of pain, numbness, or tingling in the median nerve distribution area of hand visited Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, from February 2016 to May 2017. After confirmation by physical and electrophysiological inspection, 51 patients (51 wrists) meeting the following criteria were recruited [23]: (1) pain, numbness, or tingling in the median nerve distribution area of hand, (2) nocturnal worsening of the symptoms, (3) positive Tinel and/or Phalen sign, (4) a slower median nerve conduction (SNCV≦50 m/s and/or DML≧4 ms), (5) patients with unilateral disease, and (6) the desire of the participant to have either a steroid injection or steroid injection plus MSN release. Patients were excluded from this study for the following: (1) symptomatic CTS because of diabetes, thyroid disease, or rheumatic disease, (2) cervical radiculopathy or other polyneuropathy, (3) age<18 years, (4) pregnancy, (5) steroid injection for CTS in the preceding 6 months, (6) history of wrist fracture, (7) prior carpal tunnel decompressive surgery, (8) the presence of infection or skin lesion at the site of injection, (9) patients with bilateral disease, and (10) refusal of informed consent or inability to participate in follow-up.…”