A mannosylated polymer with endosomal release properties for peptide antigen delivery

Poly(β-aminoester) Physicochemical Properties Govern the Delivery of siRNA from Electrostatically Assembled Coatings

“…Endosomal escape was reported using a HeLa reporter cell line containing a GFP-tagged galectin 8 (Gal8) . These cells were a gift from Suzie Pun’s lab at the University of Washington . The day before transfection, cells were lifted with trypsin and washed before plating at 20,000 per well in Lab-TekII 8-well chamber slides (Thermo Fisher Scientific) with 300 μL growth media.…”

“…45 These cells were a gift from Suzie Pun's lab at the University of Washington. 46 The day before transfection, cells were lifted with trypsin and washed before plating at 20,000 per well in Lab-TekII 8-well chamber slides (Thermo Fisher Scientific) with 300 μL growth media. Fresh media was added to the cells, and ∼0.3 cm 2 pieces of dressing containing siD7 with Poly2 or Poly3 were added to the wells.…”

“…73,75 Gal8 was coupled to a GFP reporter and expressed in HeLa cells (HeLa Gal8-GFP). 46 This allows fluorescence microscopic visualization of the endosomal escape process. We compared endosomal escape events in cells treated with Poly2 versus Poly3 dressings, with buffer treatment as a negative control (Figure 9A).…”

Therapeutic synthetic and natural materials for immunoengineering

“…330 Song et al recently developed a cancer vaccine consisting of synthetic mannosylation to target DCs and deliver OVA peptides. 331 The construct, named Virus-Inspired Polymers for Endosomal Release (VIPER), is assembled by increasing the pH of an aqueous buffer to physiological pH 7.4, causing the pH-responsive polymer to become deprotonated and therefore hydrophobic. Following subcutaneous injection, the authors demonstrated robust anti-cancer immunity by the reduction in B16F10-OVA tumor growth as well as strong CD8 + T Cell responses upon ex vivo re-stimulation.…”

Preparation of glycopeptide-modified pH-sensitive liposomes for promoting antigen cross-presentation and induction of antigen-specific cellular immunity

“…[10][11][12] To date, various antigen delivery systems have been reported as promoting the cytosolic delivery of an antigen, such as virus-derived fusogenic protein-introduced liposomes, cell-penetrating peptide-introduced carriers, and endosomolytic molecule-introduced carriers. [16][17][18][19] The authors have also reported several antigen delivery systems using liposomes modified with pH-responsive polymers. 20 Carboxylated polyglycidol-modified liposomes have delivered model antigenic protein (ovalbumin, OVA) successfully into cytosol of murine dendritic cell line or bone marrow-derived dendritic cells, resulting in induction of cross-presentation of OVA.…”