A Mango Leaf Extract (Zynamite®) Combined with Quercetin Has Exercise-Mimetic Properties in Human Skeletal Muscle

Abstract

Zynamite PX®, a mango leaf extract combined with quercetin, enhances exercise performance by unknown molecular mechanisms. Twenty-five volunteers were assigned to a control (17 males) or supplementation group (8 males, receiving 140 mg of Zynamite® + 140 mg quercetin/8 h for 2 days). Then, they performed incremental exercise to exhaustion (IE) followed by occlusion of the circulation in one leg for 60 s. Afterwards, the cuff was released, and a 30 s sprint was performed, followed by 90 s circulatory occlusion (same leg). Vastus lateralis muscle biopsies were obtained at baseline, 20 s after IE (occluded leg) and 10 s after Wingate (occluded leg), and bilaterally at 90 s and 30 min post exercise. Compared to the controls, the Zynamite PX® group showed increased basal protein expression of Thr287-CaMKIIδD (2-fold, p = 0.007) and Ser9-GSK3β (1.3-fold, p = 0.005) and a non-significant increase of total NRF2 (1.7-fold, p = 0.099) and Ser40-NRF2 (1.2-fold, p = 0.061). In the controls, there was upregulation with exercise and recovery of total NRF2, catalase, glutathione reductase, and Thr287-CaMKIIδD (1.2–2.9-fold, all p < 0.05), which was not observed in the Zynamite PX® group. In conclusion, Zynamite PX® elicits muscle signaling changes in resting skeletal muscle resembling those described for exercise training and partly abrogates the stress kinases responses to exercise as observed in trained muscles.

Effects of a Singular Dose of Mangiferin–Quercetin Supplementation on Basketball Performance: A Double-Blind Crossover Study of High-Level Male Players

“…Conversely, the process of acidification promotes the generation of hydroxyl radicals and diminishes the effectiveness of antioxidant enzymes [ 41 ]. Individual polyphenolic compounds such as mangiferin and quercetin possess distinct chemical characteristics that influence their specific interactions within various cellular compartments [ 42 , 43 ], and both polyphenols (mangiferin and quercetin) exhibit significant capabilities in neutralizing free radicals [ 10 , 14 ]. When utilized in combination, their cumulative antioxidant efficacy could potentially exceed that of the individual compounds [ 44 , 45 ].…”

“…This combination appears to increase muscle power in fatigued individuals, enhance peak oxygen uptake ( O 2 ), and improve brain oxygenation during extended sprinting efforts in females [ 8 ]. Mangiferin, a xanthone and non-flavonoid polyphenol, is notably abundant in mango leaves, bark, flowers, pulp, and various other plants [ 9 ] and exhibits a notable capacity for iron chelation along with potent antioxidant attributes [ 10 ]. Quercetin is a flavonoid polyphenol (occurring naturally in a variety of fruits and vegetables, including mangoes) with potential performance-enhancing properties during prolonged exercise [ 11 , 12 , 13 ] and has robust antioxidant and anti-inflammatory capabilities [ 14 ].…”

Dietary Supplements and Musculoskeletal Health and Function

“…There is also evidence that polyphenol supplementation may improve sports and exercise performance [8]. Martinez-Canton et al [9] investigated the effects of mango leaf extract (Zynamite PX ® ) combined with Quercetin supplementation on skeletal muscle NRF2 protein levels and NRF2-induced signaling under basal conditions and in response to high-intensity exercise in humans. The supplementation period lasted 48 h, and the supplementation group exhibited significant changes in resting skeletal muscle signaling, resembling those described after exercise training, and partly abrogated the stress kinase responses to exercise, as observed in trained muscles.…”