As various technologies been incorporated into education and training, the evolution of e-learning also takes its turn in many universities. In Malaysia, the evaluation of motivational dimensions of the application is considered vital as e-learning had been embedded and integrated as part of teaching and learning assistance tool in many universities. The aim of this research is to investigate the dimensions of motivation of dental students in using e-learning as part of their learning-assisted-tool. The adapted questionnaires from Website Motivational Checklist (WebMAC) professional were distributed to 144 dental students from a Malaysian public university. The motivational dimensions investigated are meaningful, stimulation, easy-to-use and organization. The outcome of the study revealed that the elearning website can be improved to increase the motivational dimensions. Some improvements on meaningful, stimulation and easy-to-use motivational dimensions are being required.