A mammalian cell-based reverse two-hybrid system for functional analysis of 3C viral protease of human enterovirus 71

Identification of Luteolin as Enterovirus 71 and Coxsackievirus A16 Inhibitors through Reporter Viruses and Cell Viability-Based Screening

“…The first approach is mechanism based and detects the enzyme activity of a purified viral protein. This approach has been developed for screening inhibitors against enteroviruses by targeting polymerase 3D pol [44] and protease 2A pro [45] and 3C pro [46]. The second approach is based on a reporter protein encoding the replicon or infectious virus, and the detected fluorescence or luminescence signal is considered to be in proportion to the virus replication.…”

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

“…Anti-EV71 strategies targeting virus entry, viral RNA-dependent RNA polymerase 3D, and viral proteases have been explored Falah et al, 2012;Hung et al, 2010;Kuo and Shih, 2013;Lee et al, 2008;Tsai et al, 2009;Zhang et al, 2012a). Compounds extracted from Chinese herbs have showed promising antiviral effect against EV71 infection (Chiang et al, 2005;Lin et al, 2009;Song et al, 2014;Zhang et al, 2014).…”

BPR-3P0128 inhibits RNA-dependent RNA polymerase elongation and VPg uridylylation activities of Enterovirus 71

“…The EV71 virion comprises several components that are potential targets for antiviral drug development, including viral capsid proteins, proteases, and proteins associated with viral RNA replication-2B, 2C, 2BC, 3A, 3B (VPg), 3AB, 3CD, and 3D (Konig and Rosenwirth, 1988;Rodriguez and Carrasco, 1993;Dragovich et al, 1998;Deszcz et al, 2004;Lee et al, 2008;Chen et al, 2008;Stone et al, 2008;Arita et al, 2010).…”