A major QTL for resistance to soil-borne cereal mosaic virus derived from an old Italian durum wheat cultivar

Rye production in European growing areas is constrained by the soilborne cereal mosaic virus (SBCMV) and the wheat spindle streak mosaic virus (WSSMV). To date, no European rye cultivars are known to exhibit resistance against these viruses. In this study, we pursued a quantitative trait locus (QTL) mapping strategy to identify genomic regions for resistance to SBCMV and WSSMV in rye. Three populations, each comprising 100 lines segregating for resistance to SBCMV and/or WSSMV, were evaluated for disease response at two years in three locations in Germany where soils are naturally infested with SBCMV and WSSMV. In the combined analysis across environments, one QTL for SBCMV resistance on chromosome 5R explained 31.9% of the phenotypic variation in one of the populations. For WSSMV resistance, one QTL explaining up to 64.0% of the phenotypic variation was detected on chromosome 7R in each of the three populations. On the Triticeae homoeologous group 5, we found evidence for synteny of the major QTL for SBCMV resistance between the wheat and rye genomes.

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“…This is supported by the findings of Russo et al. () and Maccaferri et al. (,b), who discovered up to eleven minor QTL in durum wheat for SBCMV.…”

Section: Discussionsupporting

confidence: 72%

“…,b, Russo et al. ). WSSMV resistance in bread wheat is considered to be controlled by few genes (Van Koevering et al.…”

mentioning

confidence: 99%

Oligogenic control of resistance to soil‐borne viruses SBCMV and WSSMV in rye (Secale cereale L.)

et al. 2016

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“…The wheat collection was genotyped with 26 SSR markers, which were selected based on published map data [53]–[55] according to the following criteria: locus-specific amplification, low complexity, robust amplification, and good genome coverage (nearly one marker per chromosome arm). PCR amplification was carried out in 15 µl volumes containing 2 µl DNA (≈ 80 ng), 1.5 µl 10× PCR buffer (EuroClone), 0.4 µM of each microsatellite primer (the forward primers were fluorescently labelled), 1.5 mM MgCl 2 (EuroClone), 0.2 mM dNTP mixture (Fermentas), and 1 U Taq DNA-polymerase (EuroClone).…”

Section: Methodsmentioning

confidence: 99%

Genetic Diversity and Population Structure of Tetraploid Wheats (Triticum turgidum L.) Estimated by SSR, DArT and Pedigree Data

et al. 2013

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Levels of genetic diversity and population genetic structure of a collection of 230 accessions of seven tetraploid Triticum turgidum L. subspecies were investigated using six morphological, nine seed storage protein loci, 26 SSRs and 970 DArT markers. The genetic diversity of the morphological traits and seed storage proteins was always lower in the durum wheat compared to the wild and domesticated emmer. Using Bayesian clustering (K = 2), both of the sets of molecular markers distinguished the durum wheat cultivars from the other tetraploid subspecies, and two distinct subgroups were detected within the durum wheat subspecies, which is in agreement with their origin and year of release. The genetic diversity of morphological traits and seed storage proteins was always lower in the improved durum cultivars registered after 1990, than in the intermediate and older ones. This marked effect on diversity was not observed for molecular markers, where there was only a weak reduction. At K >2, the SSR markers showed a greater degree of resolution than for DArT, with their identification of a greater number of groups within each subspecies. Analysis of DArT marker differentiation between the wheat subspecies indicated outlier loci that are potentially linked to genes controlling some important agronomic traits. Among the 211 loci identified under selection, 109 markers were recently mapped, and some of these markers were clustered into specific regions on chromosome arms 2BL, 3BS and 4AL, where several genes/quantitative trait loci (QTLs) are involved in the domestication of tetraploid wheats, such as the tenacious glumes (Tg) and brittle rachis (Br) characteristics. On the basis of these results, it can be assumed that the population structure of the tetraploid wheat collection partially reflects the evolutionary history of Triticum turgidum L. subspecies and the genetic potential of landraces and wild accessions for the detection of unexplored alleles.

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“…In particular, the DArT marker that corresponds to an NBS-LRR gene ( wPt-1601 ) represents the peak marker of a major QTL for resistance to soil-borne cereal mosaic virus that was mapped on chromosome 2B in the durum-wheat population Meridiano × Claudio [109]. Furthermore, Russo et al [110] identified two markers that corresponded to NBS-LRR genes as peak markers of minor QTLs for the same disease on chromosomes 3B and 7B in another durum-wheat population (Cirillo × Neodur). Further evidence is available in the literature relating to the involvement of some DArT markers that correspond to NBS-LRR genes in plant disease resistance.…”

Section: Nbs-lrr Genes: a Resource For Plant Breedingmentioning

confidence: 99%

Plant Nucleotide Binding Site–Leucine-Rich Repeat (NBS-LRR) Genes: Active Guardians in Host Defense Responses

Marone

Russo

Laidò

et al. 2013

IJMS

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294

221

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The most represented group of resistance genes are those of the nucleotide binding site–leucine-rich repeat (NBS-LRR) class. These genes are very numerous in the plant genome, and they often occur in clusters at specific loci following gene duplication and amplification events. To date, hundreds of resistance genes and relatively few quantitative trait loci for plant resistance to pathogens have been mapped in different species, with some also cloned. When these NBS-LRR genes have been physically or genetically mapped, many cases have shown co-localization between resistance loci and NBS-LRR genes. This has allowed the identification of candidate genes for resistance, and the development of molecular markers linked to R genes. This review is focused on recent genomics studies that have described the abundance, distribution and evolution of NBS-LRR genes in plant genomes. Furthermore, in terms of their expression, NBS-LRR genes are under fine regulation by cis- and trans-acting elements. Recent findings have provided insights into the roles of alternative splicing, the ubiquitin/proteasome system, and miRNAs and secondary siRNAs in the regulation of NBS-LRR gene expression at the post-transcriptional, post-translational and epigenetic levels. The possibility to use this knowledge for genetic improvement of plant resistance to pathogens is discussed.

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A major QTL for resistance to soil-borne cereal mosaic virus derived from an old Italian durum wheat cultivar

Cited by 16 publications

References 41 publications

Oligogenic control of resistance to soil‐borne viruses SBCMV and WSSMV in rye (Secale cereale L.)

Oligogenic control of resistance to soil‐borne viruses SBCMV and WSSMV in rye (Secale cereale L.)

Genetic Diversity and Population Structure of Tetraploid Wheats (Triticum turgidum L.) Estimated by SSR, DArT and Pedigree Data

Plant Nucleotide Binding Site–Leucine-Rich Repeat (NBS-LRR) Genes: Active Guardians in Host Defense Responses

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