A major QTL associated with preharvest sprouting in rapeseed (Brassica napus L.)

Abstract

Preharvest sprouting (PHS) is one of the most important factors affecting the cereal production worldwide, in regions characterized by rainfall and high humidity during harvest season. It is sometimes a problem in rapeseed (Brassica napus L.), especially in production of commercial F 1 hybrids. To detect quantitative trait loci (QTL) controlling PHS, a F 2 population consisting of 269 F 2:3 lines was created from the cross between a PHS-tolerant line (117AB) and a PHS-susceptible line (7,605). A linkage map was constructed using 35 Simple Sequence Repeat markers and 242 Amplified Fragment Length Polymorphism markers. PHS was measured as a percentage of sprouted seeds on the mother plant, 7 days after physiological maturity. Five putative QTLs for PHS were detected and located on LG2 (N11) and LG7 (N3), respectively. Phenotypic variance explained by each QTL ranged from 4.11 to 50.78% and the five putative QTLs explained about 75.63% of the total phenotypic variance. A major QTL was identified on LG2 (N11) flanked by P3C4180 and C6C13160, which explained 50.78% of the total phenotypic variance. Meanwhile, we detected four significant epistatic interactions with a total contribution of 17.16% of the total phenotypic variance.

Inheritance of seed quality and seed germination in two doubled haploid populations of oilseed rape segregating for acid detergent lignin (ADL) content

“…Together, this indicates that at least for this locus there is no negative pleiotropic effect of the yellow seed color alleles on pre-harvest germination. Previously, another major QTL for preharvest germination was identified on chromosome N11 of B. napus (Feng et al (2009). In the present work only a slight negative and no correlation between ADLm content and pre-harvest germination was found for the first and second population, respectively, which is in support of an independent inheritance of this trait from reduced lignin content.…”

Genetic analysis of morphological traits in a new, versatile, rapid-cycling Brassica rapa recombinant inbred line population

“…Vivipary (pre-harvest sprouting) is another important oilseed quality trait. A major QTL explaining 50.8% of the total variance for vivipary had previously been mapped to chromosome N11 of B. napus (Feng et al, 2009 ). We are not aware of previous work on seed vivipary QTLs in B. rapa .…”

Identification of two major quantitative trait locus for fresh seed dormancy using the diversity arrays technology and diversity arrays technology‐seq based genetic map in Spanish‐type peanuts

“…QTL studies reported the identification of responsible gene/QTLs in other crop species for seed dormancy/preharvest sprouting (PHS) (rapeseed, rice, Arabidopsis , wheat, barley and sorghum) (Feng et al. , Bentsink et al. , Marzougui et al.…”

“…The genetic control of preharvest sprouting or vivipary is a very important trait in many cereals and oilseed rape breeding programmes. QTL studies reported the identification of responsible gene/QTLs in other crop species for seed dormancy/preharvest sprouting (PHS) (rapeseed, rice, Arabidopsis, wheat, barley and sorghum)(Feng et al 2009, Bent-Fig. 2: Continued sink et al 2010,Marzougui et al 2012, Schatzki et al 2013, Cabral et al 2014).…”