A major mRNA of the human epididymal principal cells, HE5, encodes the leucocyte differentiation CDw52 antigen peptide backbone

Kirchhoff, Christiane; Krull, Nora; Pera, I.; Ivell, Richard

doi:10.1002/mrd.1080340103

Cited by 81 publications

(49 citation statements)

References 22 publications

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“…CD52 monoclonal antibodies are remarkably effective for complement-mediated lysis and have proved to be very potent for lymphocyte depletion both in vitro and in vivo (2,5). Differential screening of a human epididymal cDNA library yielded a highly abundant cDNA, named HE5, which turned out to be colinear to the CD52 cDNA (6). Epididymal and lymphocyte cDNAs predict the same precursor peptide, including NH 2 -terminal and COOH-terminal signals typical of GPIanchored proteins (6).…”

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confidence: 99%

“…Differential screening of a human epididymal cDNA library yielded a highly abundant cDNA, named HE5, which turned out to be colinear to the CD52 cDNA (6). Epididymal and lymphocyte cDNAs predict the same precursor peptide, including NH 2 -terminal and COOH-terminal signals typical of GPIanchored proteins (6). Both mRNAs are derived from the same single-copy gene (6,7) which has been localized on chromosome 1.…”

mentioning

confidence: 99%

“…Epididymal and lymphocyte cDNAs predict the same precursor peptide, including NH 2 -terminal and COOH-terminal signals typical of GPIanchored proteins (6). Both mRNAs are derived from the same single-copy gene (6,7) which has been localized on chromosome 1. CD52 transcripts are found in the epithelial cells of the distal epididymis and deferent duct but not in either spermatogenetic cells or on spermatozoa, nor in the more proximal parts of the epididymis (6,8).…”

mentioning

confidence: 99%

“…Both mRNAs are derived from the same single-copy gene (6,7) which has been localized on chromosome 1. CD52 transcripts are found in the epithelial cells of the distal epididymis and deferent duct but not in either spermatogenetic cells or on spermatozoa, nor in the more proximal parts of the epididymis (6,8). Anti-CD52 antibodies react strongly with parts of the male genital tract, particularly with the cauda epididymal epithelium and cauda fluid, and with epididymal but not testicular spermatozoa.…”

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Male-specific Modification of Human CD52

Schröter

Derr

Conradt³

et al. 1999

Journal of Biological Chemistry

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115

105

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CD52 is an unusually short, bipolar glycopeptide bearing a highly charged N-linked carbohydrate moiety and a glycosylphosphatidylinositol membrane anchor. It is exclusively expressed on lymphocytes and in the male genital tract where it is shed into the seminal plasma and inserts into the sperm membrane. The sperm surface molecule has potential significance as a target for antibodies that inhibit sperm function and gamete interaction. Western blot analyses suggested cell type-specific modifications of the antigen. It was purified from seminal plasma and a detailed structural analysis performed. The majority of anchor structures in male genital tract CD52 showed 2-inositol palmitoylation, rendering molecules insensitive toward phospholipase C, and a sn-1-alkyl-2-lyso-glycerol structure in place of the diacylated anchor described by Treumann et al. . N-Glycans of the male genital tract product were based on bi-, tri-, and tetraantennary structures of highly charged (up to -7), terminally sialylated complex-type sugars. A substantial proportion carried varying numbers of lactosamine repeats of which nearly 30% were branched. Different from lymphocytes, 10 -15% of all N-glycans of the male genital tract antigen also contained peripheral fucose. These data confirm that male genital tract CD52 is distinct from the lymphocyte form by both N-linked glycans and COOH-terminal attached lipid anchor.

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Male-specific Modification of Human CD52

Schröter

Derr

Conradt³

et al. 1999

Journal of Biological Chemistry

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115

105

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“…Of particular interest are the proteins that are secreted by the epididymis and interact with spermatozoa to modify their function. A number have been identified in eutherian mammals, including SPAM1 (formerly PH20; Chen et al 2006), SED1 (Ensslin and Shur 2003), α-d-mannosidase (Jin et al 1999), CRISP1 (Eberspaecher et al 1995), CD52 (Kirchhoff et al 1993), ASF (Thomas et al 1984), HE1 (Kirchhoff et al 1996) and p26H (Coutu et al 1996). However, whilst knockout mice for SPAM1 (Baba et al 2002) and SED1 (Ensslin and Shur 2003) have reduced fertility compared with wild-type, none of the knockouts is infertile.…”

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confidence: 99%

Testicular descent, sperm maturation and capacitation. Lessons from our most distant relatives, the monotremes

Ecroyd

Nixon

Dacheux

et al. 2009

Reprod. Fertil. Dev.

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The present review examines whether monotremes may help to resolve three questions relating to sperm production in mammals: why the testes descend into a scrotum in most mammals, why spermatozoa are infertile when they leave the testes and require a period of maturation in the specific milieu provided by the epididymides, and why ejaculated spermatozoa cannot immediately fertilise an ovum until they undergo capacitation within the female reproductive tract. Comparisons of monotremes with other mammals indicate that there is a need for considerable work on monotremes. It is hypothesised that testicular descent should be related to epididymal differentiation. Spermatozoa and ova from both groups share many of the proteins that are thought to be involved in gamete interaction, and although epididymal sperm maturation is significant it is probably less complex in monotremes than in other mammals. However, the monotreme epididymis is unique in forming spermatozoa into bundles of 100 with greatly enhanced motility compared with individual spermatozoa. Bundle formation involves a highly organised interaction with epididymal proteins, and the bundles persist during incubation in vitro, except in specialised medium, in which spermatozoa separate after 3 h incubation. It is suggested that this represents an early form of capacitation. Abstract. The present review examines whether monotremes may help to resolve three questions relating to sperm production in mammals: why the testes descend into a scrotum in most mammals, why spermatozoa are infertile when they leave the testes and require a period of maturation in the specific milieu provided by the epididymides, and why ejaculated spermatozoa cannot immediately fertilise an ovum until they undergo capacitation within the female reproductive tract. Comparisons of monotremes with other mammals indicate that there is a need for considerable work on monotremes. It is hypothesised that testicular descent should be related to epididymal differentiation. Spermatozoa and ova from both groups share many of the proteins that are thought to be involved in gamete interaction, and although epididymal sperm maturation is significant it is probably less complex in monotremes than in other mammals. However, the monotreme epididymis is unique in forming spermatozoa into bundles of 100 with greatly enhanced motility compared with individual spermatozoa. Bundle formation involves a highly organised interaction with epididymal proteins, and the bundles persist during incubation in vitro, except in specialised medium, in which spermatozoa separate after 2-3 h incubation. It is suggested that this represents an early form of capacitation.

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Interaction of the human epididymal protein CD52 (HE5) with epididymal spermatozoa from men and cynomolgus monkeys

Yeung¹,

Schröter²,

Wagenfeld³

et al. 1997

Mol. Reprod. Dev.

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A monoclonal antibody (CAMPATH‐1G) against the human lymphocyte surface protein CD52, which is similar to the epididymal secretion HE5, was used to ascertain the presence of this protein on maturing primate spermatozoa by flow cytometry. The percentage of human viable spermatozoa stained specifically with this antibody increased from sperm in spermatocoeles (0.5%), to the efferent ducts (3.8%), corpus (47.2%), and cauda (85.7%) epididymidis. Positive cells revealed staining mainly over the whole tail and postacrosomal region of the sperm head. Spermatozoa (∼10%) from both the efferent ducts and corpus epididymidis took up additional antigen when incubated with human distal cauda epididymidal plasma as a source of CD52, and 12–22% of human testicular sperm (from spermatocoeles) took up CD52 from human seminal plasma. In the cynomolgus monkey, nonspecific binding of control IgG was greater than that in human males and net CD52 staining was measurable only on ∼30% of corpus sperm where it was mainly on the principal piece. Neither caput nor cauda sperm took up human CD52 upon incubation with human seminal plasma, but an additional 27% of corpus sperm expressed CD52. Such uptake of CD52 was drastically reduced, or did not occur, when seminal plasma had been fractionated by filtration through 0.1 μm filters (filtrate II) or 300,000 Da cutoff filters (filtrate III), respectively. Western blots revealed that CD52 contents were much reduced in filtrate II and nondetectable in filtrate III of seminal plasma. Similar reduction of CD52 in the filtrate of cauda epididymidal plasma indicates the association of this epididymal secretion with large molecular factors and suggests their involvement as carriers in the in vivo transfer of the secretion onto the epididymal sperm surface. The in vitro uptake of CD52 by some but not all immature sperm and the detection by Western blotting of much less CD52 in the corpus than the cauda luminal plasma suggest that the acquisition of this epididymal secretion by spermatozoa depends on their maturation status as well as the availability of the protein in the epididymal lumen. Mol. Reprod. Dev. 48:267–275, 1997. © 1997 Wiley‐Liss, Inc.

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A major mRNA of the human epididymal principal cells, HE5, encodes the leucocyte differentiation CDw52 antigen peptide backbone

Cited by 81 publications

References 22 publications

Male-specific Modification of Human CD52

Male-specific Modification of Human CD52

Testicular descent, sperm maturation and capacitation. Lessons from our most distant relatives, the monotremes

Interaction of the human epididymal protein CD52 (HE5) with epididymal spermatozoa from men and cynomolgus monkeys

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