A Major Locus for Manganese Tolerance Maps on Chromosome A09 in a Doubled Haploid Population of Brassica napus L.

Raman, Harsh; Raman, Rosy; McVittie, Brett; Orchard, Beverley; Qiu, Yu; Delourme, Régine

doi:10.3389/fpls.2017.01952

Cited by 16 publications

(17 citation statements)

References 62 publications

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“…‘Darmor- bzh ’ is a French winter type and double-low oil quality cultivar, while ‘Yudal,’ is a Korean Spring type cultivar. Besides resistance to blackleg disease, this population also segregates for a range of traits such as plant height, flowering time, oil content ( Delourme et al, 2006b ) and quality, and tolerance to manganese (Mn 2+ ) toxicity ( Raman et al, 2017 ). ‘Darmor- bzh ’ carries the dwarf Bzh gene and is known to carry Rlm9 for qualitative resistance to L. maculans ( Foisset et al, 1995 ; Delourme et al, 2004 ).…”

Section: Methodsmentioning

confidence: 99%

“…Total genomic DNA was isolated following a high throughout DNA protocol, essentially based on chloroform and phenol. A framework map of the DYDH population, based on 2,094 DArTseq markers was constructed as described previously ( Raman et al, 2014 , 2017 ). PCR based SCAR (sequence characterized amplified region) marker specific to Bzh gene for dwarfness was analyzed using the primer-pairs: SCM07UP1 and LP2 as described in Barret et al (1998) .…”

Section: Methodsmentioning

confidence: 99%

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Stable Quantitative Resistance Loci to Blackleg Disease in Canola (Brassica napus L.) Over Continents

Raman

Diffey

et al. 2018

Front. Plant Sci.

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The hemibiotrophic fungus, Leptosphaeria maculans is the most devastating pathogen, causing blackleg disease in canola (Brassica napus L). To study the genomic regions involved in quantitative resistance (QR), 259–276 DH lines from Darmor-bzh/Yudal (DYDH) population were assessed for resistance to blackleg under shade house and field conditions across 3 years. In different experiments, the broad sense heritability varied from 43 to 95%. A total of 27 significant quantitative trait loci (QTL) for QR were detected on 12 chromosomes and explained between 2.14 and 10.13% of the genotypic variance. Of the significant QTL, at least seven were repeatedly detected across different experiments on chromosomes A02, A07, A09, A10, C01, and C09. Resistance alleles were mainly contributed by ‘Darmor-bzh’ but ‘Yudal’ also contributed few of them. Our results suggest that plant maturity and plant height may have a pleiotropic effect on QR in our conditions. We confirmed that Rlm9 which is present in ‘Darmor-bzh’ is not effective to confer resistance in our Australian field conditions. Comparative mapping showed that several R genes coding for nucleotide-binding leucine-rich repeat (LRR) receptors map in close proximity (within 200 Kb) of the significant trait-marker associations on the reference ‘Darmor-bzh’ genome assembly. More importantly, eight significant QTL regions were detected across diverse growing environments: Australia, France, and United Kingdom. These stable QTL identified herein can be utilized for enhancing QR in elite canola germplasm via marker- assisted or genomic selection strategies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Stable Quantitative Resistance Loci to Blackleg Disease in Canola (Brassica napus L.) Over Continents

Raman

Diffey

et al. 2018

Front. Plant Sci.

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“…One of the highly significant association peaks was located close to the BnMn 2+ .A09 locus, previously identified by QTL analysis in the B. napus DH population (Raman et al, 2017). However, no QTL/eQTL for Mn 2+ tolerance on C03 and C09 chromosomes were identified in earlier studies.…”

Section: Bnmtp8a09 Controls Natural Variation For Mn 2+ Tolerance In ...mentioning

confidence: 79%

“…Natural variation for tolerance to Mn 2+ toxicity is described in several plant species, including Brassica napus (Foy, 1984, Horiguchi, 1988, Khan and McNeilly, 1998, Basu et al, 2001, Schaaf et al, 2002, Kassem et al, 2004, Peiter et al, 2007, Mizuno et al, 2008, Pradeep et al, 2020, Wratten and Scott, 1979, Moroni et al, 2003, Delhaize et al, 2003, Raman et al, 2017. Studies have shown that AtMTP11, BnMTP8.C04, BnMTP9.A07, ShMTP1, and NRAMP5 confer tolerance to Mn 2+ tolerance in plants (Gu et al, 2022, Delhaize et al, 2003, Delhaize et al, 2007, Noor et al, 2023.…”

Section: These Include Various Transporters Such As Natural Resistanc...mentioning

confidence: 99%

Genome-wide association study elucidates the genetic architecture of manganese tolerance inBrassica napus

Raman,

Bai,

McVittie

et al. 2024

Preprint

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Brassica napus (canola) is a significant contributor to the world's oil production and is cultivated across continents, yet acidic soils with Al3+ and Mn2+ toxicities limit its production. The genetic determinants underlying acidic soil tolerance in canola are unknown and require to be uncovered for canola breeding and production. Here, through comprehensive phenotyping, whole genome resequencing, and genome-wide association analysis, we identified three QTLs for tolerance to Mn2+ toxicity on chromosomes A09, C03, and C09. Allelism tests between four tolerance sources confirmed that at least one locus on A09 controls Mn2+ tolerance in B. napus. Integrated analysis of genomic and expression QTL and Mn2+ tolerance data reveals that BnMTP8.A09, in conjunction with BnMATE.C03, BnMTP8.C04 and BnMTP8.C08, play a central role in conferring Mn2+ tolerance in B. napus. Gene expression analysis revealed a high correlation (R2 = 0.74) between Mn2+ tolerance and the BnMTP8.A09 expression. Yeast complementation assays show that BnMTP8.A09 can complement manganese-hypersensitive yeast mutant strain PMR1Δ and restore Mn2+ tolerance to wild-type levels. Inductively coupled plasma mass spectrometry revealed that Mn2+ tolerant accessions accumulate less Mn in the shoots compared to Mn2+ sensitives, suggesting that the BnMTP8.A09 transporter likely sequesters Mn2+ into the tonoplast. Taken together, our research unveils the genetic architecture of Mn2+ tolerance and identifies BnMTP8.A09 as a major gene imparting tolerance to Mn2+ toxicity in B. napus.

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“…Only few studies have addressed genetic factors with Mn tolerance in plants (Wang et al, 2002;Kassem et al, 2004;Raman et al, 2017;Huang et al, 2018;Shrestha et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Genome Wide Association Analysis of Plant Height, Spike and Awn Length in Barley (Hordeum Vulgare L.) Exposed to Mn Stress

Koochakpour¹,

Solouki²,

Fakheri³

et al. 2020

THE JAPS

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Plant productivity is influenced by environmental conditions. Abiotic stresses limit yield potential in crops. Mn toxicity is an important constraint to crop production. In this study, whole genome association mapping was used in a spring barley population with 148 diverse genotypes for morphological traits underlying Mn stress. Association analysis between markers and phenotypic traits was performed with a mixed linear model (MLM with K+Q). A total of 39 significant markers were identified under both non stress and stress conditions. These markers were found on chromosomes 1H, 2H, 3H, 4H and 5H. Many of the associated markers were located in regions where QTL had previously been identified. Three significant markers on chromosomes 3H and 5H were associated with main spike length (MSL) and awn length (AL). Thus, it can be proved that MSL and AL have the same controller genomic regions. The results indicated that Mn tolerance was quantitatively inherited, and the detected QTLs may be useful for markerassistant selection and identification of the genes controlling Mn tolerance in barley.

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A Major Locus for Manganese Tolerance Maps on Chromosome A09 in a Doubled Haploid Population of Brassica napus L.

Cited by 16 publications

References 62 publications

Stable Quantitative Resistance Loci to Blackleg Disease in Canola (Brassica napus L.) Over Continents

Stable Quantitative Resistance Loci to Blackleg Disease in Canola (Brassica napus L.) Over Continents

Genome-wide association study elucidates the genetic architecture of manganese tolerance inBrassica napus

Genome Wide Association Analysis of Plant Height, Spike and Awn Length in Barley (Hordeum Vulgare L.) Exposed to Mn Stress

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