Galaxy clusters trace the largest structures of the Universe and provide ideal laboratories for studying galaxy evolution and cosmology 1,2 . Clusters with extended Xray emission have been discovered at redshifts up to z ≈ 2.5 3-7 . Meanwhile, there has been growing interest in hunting for protoclusters, the progenitors of clusters, at higher redshifts [8][9][10][11][12][13][14] . It is, however, very challenging to find the largest protoclusters at early times when they start to assemble. Here we report a giant protocluster of galaxies at 2 redshift z ≈ 5.7, when the Universe was only one billion years old. This protocluster occupies a volume of about 35 3 cubic co-moving megaparsecs (cMpc 3 ). It is embedded in an even larger overdense region with at least 41 spectroscopically confirmed, luminous Lyα-emitting galaxies (Lyα Emitters, or LAEs), including several previously reported LAEs 9 . Its LAE density is 6.6 times the average density at z ≈ 5.7. It is the only one of its kind in a LAE survey in four square degrees on the sky. Such a large structure is also rarely seen in current cosmological simulations. This protocluster will collapse into a galaxy cluster with a mass of (3.6 ± 0.9) × 10 15 solar masses (M⨀), comparable to those of the most massive clusters or protoclusters known to date.According to cosmological simulations, the largest protoclusters of galaxies extend over tens of co-moving megaparsecs at z > 5 (refs. 15,16). Deep, wide-area surveys are needed to find these giant structures at high redshift. We are carrying out a spectroscopic survey of galaxies in four square degrees on the sky, aiming to build a large and homogeneous sample of LAEs at z ≈ 5.7 and 6.5. We are observing five well-studied fields, including the Subaru XMM-Newton Deep Survey (SXDS) field 17 . These fields have deep optical imaging data in a series of broad and narrow bands, taken by the prime-focus imager Suprime-Cam on the 8.2m Subaru telescope. The combined SXDS images in five broad-band filters (BVRi'z') and two narrow-band filters (NB816 and NB921) have enabled us to efficiently select LAE candidates at z ≈ 5.7 and 6.5 via the Lyα technique 18-21 . From these LAE candidates, we identified a large overdense region at z ≈ 5.7 in the SXDS. Here we show that this overdense region contains a giant protocluster (SXDS_gPC for short) that will grow into a massive galaxy cluster.We carried out deep spectroscopic observations of SXDS_gPC using the fiber-fed, multi-object spectrograph M2FS 22 on the 6.5-m Magellan Clay telescope. M2FS has 256 optical fibers deployed over a circular field-of-view 29.2 arcminutes in diameter. SXDS_gPC and its surrounding environment were covered by one M2FS pointing that included z ≈ 5.7 LAE candidates brighter than NB816 = 25.8 mag (5σ detection for point sources; magnitudes are on the AB system), as well as a variety of galaxy candidates at other redshifts. We used a pair of red-sensitive gratings with a resolving power of about 2000. We obtained 7 hours of on-source integration (7 one-hour individ...