A macrophage/fibroblast co-culture system using a cell migration chamber to study inflammatory effects of biomaterials

Zhou, Guoying; Loppnow, Harald; Groth, Thomas

doi:10.1016/j.actbio.2015.08.020

Cited by 41 publications

(39 citation statements)

References 80 publications

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“…Physically etched polymers via chloroform resulted in decreased proinflammatory and increased anti‐inflammatory cytokine secretion in both fibroblasts and macrophages, along with the highest levels of collagen and elastin production by fibroblasts. To add an additional element of complexity, another model was developed to combine self‐assembled monolayer biomaterials with various changes in surface wettability and charge with cell culture fence chambers with internal and external compartments that facilitated cocultures of THP1‐derived macrophages and fibroblasts . Hydrophobic surfaces prepared with methyl (CH 3 )‐terminated groups yielded the most significant proinflammatory response in macrophage‐fibroblast cocultures, while hydrophilic/anionic surfacesprepared with carboxylic acid (COOH)‐terminated groups resulted in significantly diminished inflammatory response .…”

Section: Macrophage–fibroblast Crosstalk In Vitromentioning

confidence: 99%

Macrophage and Fibroblast Interactions in Biomaterial‐Mediated Fibrosis

Witherel

Abebayehu

Barker

et al. 2019

Adv Healthcare Materials

254

201

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Biomaterial‐mediated inflammation and fibrosis remain a prominent challenge in designing materials to support tissue repair and regeneration. Despite the many biomaterial technologies that have been designed to evade or suppress inflammation (i.e., delivery of anti‐inflammatory drugs, hydrophobic coatings, etc.), many materials are still subject to a foreign body response, resulting in encapsulation of dense, scar‐like extracellular matrix. The primary cells involved in biomaterial‐mediated fibrosis are macrophages, which modulate inflammation, and fibroblasts, which primarily lay down new extracellular matrix. While macrophages and fibroblasts are implicated in driving biomaterial‐mediated fibrosis, the signaling pathways and spatiotemporal crosstalk between these cell types remain loosely defined. In this review, the role of M1 and M2 macrophages (and soluble cues) involved in the fibrous encapsulation of biomaterials in vivo is investigated, with additional focus on fibroblast and macrophage crosstalk in vitro along with in vitro models to study the foreign body response. Lastly, several strategies that have been used to specifically modulate macrophage and fibroblast behavior in vitro and in vivo to control biomaterial‐mediated fibrosis are highlighted.

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Section: Macrophage–fibroblast Crosstalk In Vitromentioning

confidence: 99%

Macrophage and Fibroblast Interactions in Biomaterial‐Mediated Fibrosis

Witherel

Abebayehu

Barker

et al. 2019

Adv Healthcare Materials

254

201

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“…In a clinical setting, the effectiveness of heparin can be monitored with coagulation tests such as activated clotting time or thrombin time (Bjornsson et al, ). More complex trials and the combination of different cell types are used to investigate multifaceted process such as inflammation (Zhou et al, ).…”

Section: Chemical Alteration and Bioactivity Of Modified Glycosaminogmentioning

confidence: 99%

Medical application of glycosaminoglycans: a review

Köwitsch

Zhou

Groth

2017

J Tissue Eng Regen Med

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180

145

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The characteristic molecular composition of the different glycosaminoglycans (GAGs) is related to their role as structural components and regulators of a multitude of functions of proteins, cells and tissues in the human body. Therefore, it is not surprising that GAGs are widely used as coating materials for implants, components of 3D-constructs such as tissue engineering scaffolds and hydrogels, but also as diagnostic devices such as biosensors and in controlled release applications. Beside a physisorption or encapsulation of GAGs, these applications often require their chemical modification to allow a stable covalent attachment on surfaces or cross-linking reactions with other molecules. Then, the preservation of the functionality of GAGs under maintenance of their biocompatibility is a challenging task and must be addressed in accordance with the designated field of application. Here, we will give a brief overview on structure and biological functions of GAGs, different methods of their activation and immobilization, the recent progress in GAG-related biomaterials development, as well as some examples of their application in the field of tissue engineering and regenerative medicine.

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“…Because of the key effects of monocytes/macrophages in regulation of inflammation, different in vitro monocytes/ macrophages-based cell models have been used to study inflammation and FBR. 15,16 Among which, the THP-1 cell lines were often applied as model monocyte systems owing to their uniform genetic background with no absent donor variation, 17 as well as their effective differentiation into macrophage-like cells by treatment with phorbol-12myristate-13-acetate (PMA). 18 Hence, by using the THP-1derived macrophages, the current study aims to evaluate the anti-inflammatory effects of multilayers composed of different GAGs as polyanions and Chi as polycation in a simple in vitro setting.…”

Section: Introductionmentioning

confidence: 99%

Reducing the inflammatory responses of biomaterials by surface modification with glycosaminoglycan multilayers

Zhou

Niepel

Saretia

et al. 2015

J Biomedical Materials Res

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Chronic inflammatory responses after implantation of biomaterials can lead to fibrotic encapsulation and failure of implants. The present study was designed to reduce the inflammatory responses to biomaterials by assembling polyelectrolyte multilayers (PEMs) composed of glycosaminoglycans (GAGs) and chitosan (Chi) on glass as model surfaces through layer-by-layer (LBL) technique. Surface plasmon resonance (SPR) and water contact angle (WCA) investigations confirmed the multilayer build-up with alternating deposition of GAGs and Chi layers, while zeta potential measurements showed significant negative charges after multilayer deposition, which further proved the PEM formation. Macrophage adhesion, macrophage spreading morphology, foreign body giant cell (FBGC) formation, as well as β1 integrin expression and interleukin-1β (IL-1β) production were all significantly decreased by GAG-Chi multilayer deposition in comparison to the primary poly (ethylene imine) (PEI) layer. Thereby, the type of GAGs played a pivotal role in inhibiting the inflammatory responses to various extents. Especially heparin (Hep)-Chi multilayers hindered all inflammatory responses to a significantly higher extent in comparison to hyaluronic acid (HA)-Chi and chondroitin sulfate (CS)-Chi multilayer systems. Overall, the present study suggests a great potential of GAG-Chi multilayer coating on implants, particularly the Hep-Chi based systems, to reduce the inflammatory responses.

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A macrophage/fibroblast co-culture system using a cell migration chamber to study inflammatory effects of biomaterials

Cited by 41 publications

References 80 publications

Macrophage and Fibroblast Interactions in Biomaterial‐Mediated Fibrosis

Macrophage and Fibroblast Interactions in Biomaterial‐Mediated Fibrosis

Medical application of glycosaminoglycans: a review

Reducing the inflammatory responses of biomaterials by surface modification with glycosaminoglycan multilayers

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