A LysR-Type Transcriptional Regulator LcrX Is Involved in Virulence, Biofilm Formation, Swimming Motility, Siderophore Secretion, and Growth in Sugar Sources in Xanthomonas axonopodis Pv. glycines

Park, Hanbi; Do, Eunsoo; Kim, Minyoung; Park, Hye-Jee; Lee, Jong-Chan; Han, Sang-Wook

doi:10.3389/fpls.2019.01657

Cited by 21 publications

(20 citation statements)

References 87 publications

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“…The first transcriptional factor described that specifically responds to HOCl, YjiE, belongs to the family LysR-type transcriptional regulator (LTTR) [ 111 ]. LTTR, the largest family of transcriptional regulators described in prokaryotes, is composed of a helix-turn-helix (HTH) on its DNA binding domain (DBD) and a regulatory domain (RD) that can activate or repress gene expression [ 112 ]. It regulates the transcription of several genes, including those involved in adherence [ 113 ], efflux pump [ 114 ], antibiotic resistance [ 115 ], biofilm formation [ 116 ], and oxidative stress response [ 117 ].…”

Section: Adaptive Response Of Gram-negative Cells To Hoclmentioning

confidence: 99%

Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid

2020

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Sodium hypochlorite (NaOCl) and its active ingredient, hypochlorous acid (HOCl), are the most commonly used chlorine-based disinfectants. HOCl is a fast-acting and potent antimicrobial agent that interacts with several biomolecules, such as sulfur-containing amino acids, lipids, nucleic acids, and membrane components, causing severe cellular damage. It is also produced by the immune system as a first-line of defense against invading pathogens. In this review, we summarize the adaptive responses of Gram-negative bacteria to HOCl-induced stress and highlight the role of chaperone holdases (Hsp33, RidA, Cnox, and polyP) as an immediate response to HOCl stress. We also describe the three identified transcriptional regulators (HypT, RclR, and NemR) that specifically respond to HOCl. Besides the activation of chaperones and transcriptional regulators, the formation of biofilms has been described as an important adaptive response to several stressors, including HOCl. Although the knowledge on the molecular mechanisms involved in HOCl biofilm stimulation is limited, studies have shown that HOCl induces the formation of biofilms by causing conformational changes in membrane properties, overproducing the extracellular polymeric substance (EPS) matrix, and increasing the intracellular concentration of cyclic-di-GMP. In addition, acquisition and expression of antibiotic resistance genes, secretion of virulence factors and induction of the viable but nonculturable (VBNC) state has also been described as an adaptive response to HOCl. In general, the knowledge of how bacteria respond to HOCl stress has increased over time; however, the molecular mechanisms involved in this stress response is still in its infancy. A better understanding of these mechanisms could help understand host-pathogen interactions and target specific genes and molecules to control bacterial spread and colonization.

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Section: Adaptive Response Of Gram-negative Cells To Hoclmentioning

confidence: 99%

Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid

2020

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“…Xag can be spread by rain droplets during the growing season and by seed transportation 7 . A number of spreads of bacterial pustule disease have taken place in Asia during recent years, reducing the size and quantity of the seeds 8,9 …”

Section: Introductionmentioning

confidence: 99%

“…7 A number of spreads of bacterial pustule disease have taken place in Asia during recent years, reducing the size and quantity of the seeds. 8,9 Flavonoids rutin and genistein have been directly related to the defence mechanism of soybean to Xag, and soybean resistant varieties are known to produce higher amounts of flavonoids in comparison with susceptible varieties. 10,11 Although soybean has been reported to produce Biochanin A, the concentration of this isoflavonoid is under the quantification limits, indicating that soybean can synthesize Biochanin A only in very low concentration.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial mechanism of Biochanin A and its efficacy for the control of Xanthomonas axonopodispv.glycines in soybean

Shi

et al. 2020

Pest Management Science

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BACKGROUND Xanthomonas axonopodis pv. glycines (Xag) is a hazardous pathogen able to cause bacterial pustule disease in soybean, reducing crop yield and quality. Although flavonoids rutin and genistein are known to play an important role in soybean defence, soybean is only able to produce Biochanin A in low concentration. RESULTS In this work, Biochanin A was found to produce higher antibacterial activity against Xag in comparison with genistein (minimum inhibitory concentration < 100 μg/mL). Biochanin A was able to inhibit DNA synthesis and flagella formation in Xag, and altered the composition of the bacterial membrane. These effects reduced swimming motility, extracellular protease activity and biofilm formation. Further, Biochanin A was tested for the control of Xag in soybean leaves, showing similar, or even higher, inhibitory ability in comparison with some products commonly used for the control of this pathogen. CONCLUSIONS The antibacterial properties of Biochanin A against Xag have been studied for the first time, revealing new insights on the potential applications of this isoflavonoid for the management of bacterial pustule disease. © 2020 Society of Chemical Industry

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“…However, among the final 22 immunogenic proteins, only MbovP274 was unique to P1. Although it is highly immunogenic, the conserve domain database (CDD) prediction showed that this protein contains a highly conserved LysR transcriptional regulatory protein family domain that is involved in virulence and biofilm formation [ 24 ]. MbovP296 and MbovP518 are unique to P150.…”

Section: Discussionmentioning

confidence: 99%

In Silico Identification of Novel Immunogenic Secreted Proteins of Mycoplasma bovis from Secretome Data and Experimental Verification

et al. 2020

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Mycoplasma bovis is a major pathogen, responsible for bovine respiratory diseases worldwide. The present lack of effective control measures leaves cattle owners at considerable perpetual risk of M. bovis outbreaks. In this study, we identified M. bovis secreted immunogenic proteins in silico as potential candidates for novel diagnostic agents and vaccines. We used immunoinformatics to analyze 438 M. bovis proteins previously identified with a label-free proteomics analysis of virulent M. bovis HB0801 (P1) and its attenuated P150 strains. The subcellular localization of these proteins was preliminarily screened and 59 proteins were found to be secreted extracellular proteins. Twenty-seven of these proteins contained a large number of predictive T-cell epitopes presented by major histocompatibility complex (MHC) class I and II molecules. Twenty-two of these 27 proteins had a high number of conformational B-cell epitopes, predicted from the corresponding 3D structural templates, including one unique to P1, two unique to P150, and 19 common to both strains. Five proteins were selected for further validation, and two of these, MbovP274 and MbovP570, were successfully expressed and purified. Both were confirmed to be secretory and highly immunogenic proteins that induced a mouse antibody response, reacted with cattle serum positive for M. bovis infection, and significantly increased the production of interleukin 8 (IL-8), IL-12 and interferon γ (IFN-γ) during the secretion of these three cytokines by both M. bovis mutants of these genes. These results should be useful in the development of novel immunological agents against M. bovis infection.

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A LysR-Type Transcriptional Regulator LcrX Is Involved in Virulence, Biofilm Formation, Swimming Motility, Siderophore Secretion, and Growth in Sugar Sources in Xanthomonas axonopodis Pv. glycines

Cited by 21 publications

References 87 publications

Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid

Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid

Antibacterial mechanism of Biochanin A and its efficacy for the control of Xanthomonas axonopodispv.glycines in soybean

In Silico Identification of Novel Immunogenic Secreted Proteins of Mycoplasma bovis from Secretome Data and Experimental Verification

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