A Lysosome-Targetable Fluorescent Probe for Simultaneously Sensing Cys/Hcy, GSH, and H₂S from Different Signal Patterns

Abstract

Biothiols, a vital branch of reactive sulfur species (RSS) family, are indispensable in human physiology. However, the exact functional roles of each biothiol involved in complicated physiological activities are still not fully clarified. A critical barrier is a lack of robust molecular tools which can simultaneously visualize different biothiols with distinct emission signals. Herein, the first lysosome-targetable fluorescent probe, Lyso-RC, which could respond to Cys/ Hcy, GSH, and H 2 S with different sets of signal patterns was developed. Lyso-RC responds to Cys/Hcy, GSH, and H 2 S with the fluorescence signal patterns of blue-red, green-red, and red, respectively. Significantly, Lyso-RC is capable of discriminating lysosomal Cys/Hcy, GSH, and H 2 S in HeLa cells.

Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging

“…The solvents were dried according to standard procedures, aqueous solutions were prepared using deionized water in the test. 1 H NMR and 13 C NMR spectra were recorded on a Bruker AV-400 MHz spectrometer, and HR-MS spectra were measured using an Agilent 1290-micrOTOF Q II instrument in the ESI mode. Fluorescence measurement was performed on a Hitachi F-4500 spectrometer with a quartz cell (1 cm  1 cm) and UV-vis absorption spectra were obtained on a Hitachi U-3010©.…”

“…In recent years, various methods (including highperformance liquid chromatography, 7,8 mass spectrometry, 9,10 capillary electrophoresis, 11 optical sensing, [12][13][14][15] and electrochemical methods 16 ) for detecting Cys have been proposed on the basis of the close relationship between endogenous Cys and health of living organisms. However, traditional methods have inevitable disadvantages, such as high detection cost, long detection time, complicated pretreatment, and limited applications in vivo.…”

A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the ‘Naked–Eye’ Detection of Biothiols in Human Urine

“…Biothiols, such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play critical roles in cellular processes and are always involved in physiological and pathological actions [1][2][3]. It is widely reported that cysteine (Cys) and homocysteine (Hcy) are associated with cellular growth, while glutathione (GSH) is mainly related to cellular homeostasis [4].…”

A Simple Long-wavelength Fluorescent Probe for Simultaneous Discrimination of Cysteine/Homocysteine and Glutathione/Hydrogen Sulfide with Two Separated Fluorescence Emission Channels by Single Wavelength Excitation

“…Many probes were designed and synthesized to detect biothiols through different chemical reaction mechanisms. [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48] However, two main design strategies have been widely used. One is to use the nucleophilicity, reductivity and metal ion complexation ability of the mercapto group in the biothiol molecule to react with the probe molecule.…”