A lysine sensor for process control

Abstract

A lysine sensor for process control of lysine fermentation was developed based on a Clark‐type electrode in combination with L‐lysine‐α‐oxidase. The enzyme, isolated from Trichoderma viride, was immobilized between a cellulose and a polypropylene foil using a polyurethane resin. Lysine determinations were carried out in a flow‐through system as anodic measurements when H2O2 was measured and as chathodic measurements when the consumption of O2 was followed. The sensitivity of the sensor toward other amino acids was determined.

Graphene/Poly(vinylferrocene) Composite Based Amperometric Biosensor for L‐lysine Determination

“…Siegler et al. , reported 90 to 28 % interference from L‐ornithine, depending on the L‐ornithine concentration. Saurina et al., determined the interference of ornithine, tyrosine, arginine and glutamic acid as 42 %, 34 %, 13.1 %, 14.7 % and 10.1 %, respectively .…”

A novel amperometric biosensor based on a co-crosslinked l-lysine-α-oxidase/overoxidized polypyrrole bilayer for the highly selective determination of l-lysine

“…Indeed, the interfering effect of ornithine and arginine is surely the basic problem of the selectivity of most l-lysine biosensing systems based on l-lysine-␣-oxidase from T. viride [17]. For l-ornithine, for example, typical interference bias ranges from 28% up to 90% [4,23,25] whereas interference from l-arginine on l-lysine response was of 15% [23]. To overcome the poor enzyme specificity, other approaches were therefore proposed.…”

“…Owing to this, many efforts have been devoted to devise simple and sensitive analytical methods spontaneously. This reaction is usually monitored amperometrically by measuring either the consumption of oxygen with Clark-type electrodes [1][2][3][4] or the formation of hydrogen peroxide mainly with platinum electrodes [5][6][7]. Anyway, these enzyme-based analytical approaches are not without drawbacks.…”

Development of Small-sized Lysine Enzyme Sensor for Clinical Use

“…Four different enzymes have been used to develop biosensor systems for l-lysine analysis: l-lysinedecarboxylase, (6,7) l-lysine-dehydrogenase, (8) l-lysine-2-monooxygenase, (9) and l-lysine-(α)oxidase. (10)(11)(12)(13)(14)(15)(16)(17)(18)(19) l-lysine-decarboxylase was used with a CO 2 sensor to determine the lysine concentration in aqueous solutions. l-lysine-dehydrogenase was used for a flow injection analysis system and showed a high throughput of 40 samples/h.…”