A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans

Rivas, Mariella; Seeger, Michael; Holmes, David S.; Jedlicki, Eugenia

doi:10.4067/s0716-97602005000200018

Cited by 57 publications

(56 citation statements)

References 48 publications

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“…Interestingly, A. ferrooxidans harbors two QS systems (426). A Lux-like system is upregulated when A. ferrooxidans is grown in sulfur medium (427,428), while an Act-based system is upregulated when A. ferrooxidans is grown in medium containing iron instead of sulfur (426). Thus, it has been suggested that the two QS systems respond to different environmental signals that may be related to the abilities of A. ferrooxidans to colonize and use different solid sulfur-and ironcontaining minerals (426).…”

Section: Microbial Quorum Sensingmentioning

confidence: 99%

Microbial Surface Colonization and Biofilm Development in Marine Environments

Dang

Lovell

2016

Microbiol Mol Biol Rev

799

636

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SUMMARYBiotic and abiotic surfaces in marine waters are rapidly colonized by microorganisms. Surface colonization and subsequent biofilm formation and development provide numerous advantages to these organisms and support critical ecological and biogeochemical functions in the changing marine environment. Microbial surface association also contributes to deleterious effects such as biofouling, biocorrosion, and the persistence and transmission of harmful or pathogenic microorganisms and their genetic determinants. The processes and mechanisms of colonization as well as key players among the surface-associated microbiota have been studied for several decades. Accumulating evidence indicates that specific cell-surface, cell-cell, and interpopulation interactions shape the composition, structure, spatiotemporal dynamics, and functions of surface-associated microbial communities. Several key microbial processes and mechanisms, including (i) surface, population, and community sensing and signaling, (ii) intraspecies and interspecies communication and interaction, and (iii) the regulatory balance between cooperation and competition, have been identified as critical for the microbial surface association lifestyle. In this review, recent progress in the study of marine microbial surface colonization and biofilm development is synthesized and discussed. Major gaps in our knowledge remain. We pose questions for targeted investigation of surface-specific community-level microbial features, answers to which would advance our understanding of surface-associated microbial community ecology and the biogeochemical functions of these communities at levels from molecular mechanistic details through systems biological integration.

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Section: Microbial Quorum Sensingmentioning

confidence: 99%

Microbial Surface Colonization and Biofilm Development in Marine Environments

Dang

Lovell

2016

Microbiol Mol Biol Rev

799

636

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“…2). Interestingly, an open reading frame similar to A1S_0110 was present immediately upstream of putative autoinducer synthases from A. ferrooxidans (37% identity and 48% similarity) and Burkholderia pseudomallei (38% identity and 62% similarity) (9,33). Based on the 17989 genome, an open reading frame divergently transcribed from the A1S_0110 and abaI open reading frames was predicted to encode a LuxR protein designated AbaR (Fig.…”

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confidence: 99%

Isolation and Characterization of an Autoinducer Synthase from Acinetobacter baumannii

et al. 2008

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The opportunistic human pathogen Acinetobacter baumannii strain M2 was found to produce distinct acyl-homoserine lactone (AHL) signals based on the use of an Agrobacterium tumefaciens traG-lacZ biosensor. An A. baumannii gene, designated abaI, was cloned and directed AHL production in recombinant Escherichia coli. The AbaI protein was similar to members of the LuxI family of autoinducer synthases and was predicted to be the only autoinducer synthase encoded by A. baumannii. The primary AHL signal directed by AbaI was identified by mass spectrometry as being N-(3-hydroxydodecanoyl)-L-HSL (3-hydroxy-C 12 -HSL). Minor amounts of at least five additional AHLs were also identified. The expression of abaI at the transcriptional level was activated by ethyl acetate extracts of culture supernatants or by synthetic 3-hydroxy-C 12 -HSL. An abaI::Km mutant failed to produce any detectable AHL signals and was impaired in biofilm development.

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“…The genome of A. ferrooxidans was searched for genes with similarity to all known genes involved in QS, and apart from the previously reported luxIR gene pair (8,27) a significant hit was found only to hdtS (gi.11120074) of P. fluorescens F113 (15). hdtS has been shown to encode an AHL synthase responsible for the production of AHLs of various chain lengths.…”

Section: Resultsmentioning

confidence: 99%

“…Little is known about QS systems in other extremophilic archaea, although a genome-wide survey indicated that a furanosyl-borate diester signaling system may be prevalent in archaea (38). The paucity of information regarding bacterial communication in extreme conditions, especially in acidic environments, prompted the present investigation.Recently, a classic LuxIR system was described in the extreme acidophile Acidithiobacillus ferrooxidans (8,27). A. ferrooxidans is a chemolithoautotrophic, mesophilic, facultative aerobe of the gammaproteobacterium group.…”

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confidence: 99%

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Second Acyl Homoserine Lactone Production System in the Extreme AcidophileAcidithiobacillus ferrooxidans

Rivas

Seeger

Jedlicki

et al. 2007

Appl Environ Microbiol

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The acidophilic proteobacterium Acidithiobacillus ferrooxidans is involved in the industrial biorecovery of copper. It is found in acidic environments in biofilms and is important in the biogeochemical cycling of metals and nutrients. Its genome contains a cluster of four genes, glyQ, glysS, gph, and act, that are predicted to encode the ␣ and ␤ subunits of glycine tRNA synthetase, a phosphatase, and an acyltransferase, respectively (GenBank accession no. DQ149607). act, cloned and expressed in Escherichia coli, produces acyl homoserine lactones (AHLs) principally of chain length C 14 according to gas chromatography and mass spectrometry measurements. The AHLs have biological activity as shown by in vivo studies using the reporter strain Sinorhizobium meliloti Rm41 SinI ؊ . Reverse transcription-PCR (RT-PCR) experiments indicate that the four genes are expressed as a single transcript, demonstrating that they constitute an operon. According to semiquantitative RT-PCR results, act is expressed more highly when A. ferrooxidans is grown in medium containing iron than when it is grown in medium containing sulfur. Since AHLs are important intercellular signaling molecules used by many bacteria to monitor their population density in quorum-sensing control of gene expression, this result suggests that A. ferrooxidans has two quorum-sensing systems, one based on Act, as described herein, and the other based on a Lux-like quorum-sensing system, reported previously. The latter system was shown to be upregulated in A. ferrooxidans grown in sulfur medium, suggesting that the two quorum-sensing systems respond to different environmental signals that may be related to their abilities to colonize and use different solid sulfur-and iron-containing minerals.Quorum sensing (QS) is a process by which bacteria communicate via the secretion and detection of chemical signaling molecules termed autoinducers. It is an important method for the regulation of population density-dependent cellular processes, such as the production of antibiotics and virulence factors, conjugation, transformation, swarming behavior, and biofilm formation (20). Several different QS systems have been discovered including the LuxIR paradigm, in which the signaling molecule is an acyl homoserine lactone (AHL), used principally by gram-negative bacteria (22,39,40); the furanosylborate diester signaling system (3); the peptide signaling systems used primarily by gram-positive bacteria (7); the LuxS/ autoinducer-2 signaling used for interspecies communication; and the 3/epinephrine/norepinephrine interkingdom signaling system (36).Although QS systems have been described in a wide variety of microorganisms (26), there are few reports of the presence of QS in extremophiles. AHL-based QS has been detected in the haloalkaliphilic archaeon Natronococcus occultus (21) and in the haloalkaliphilic Halomonas genus of bacteria (17). In addition, peptide-based QS has been detected in the hyperthermophilic bacterium Thermotoga maritima (13). Little is known about QS systems i...

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A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans

Cited by 57 publications

References 48 publications

Microbial Surface Colonization and Biofilm Development in Marine Environments

Microbial Surface Colonization and Biofilm Development in Marine Environments

Isolation and Characterization of an Autoinducer Synthase from Acinetobacter baumannii

Second Acyl Homoserine Lactone Production System in the Extreme AcidophileAcidithiobacillus ferrooxidans

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