A luminescent hybridoma-based biosensor for rapid detection of V. cholerae upon induction of calcium signaling pathway

Zamani, Parichehr; Sajedi, Reza H.; Hosseinkhani, Saman; Zeinoddini, Mehdi; Bakhshi, Bita

doi:10.1016/j.bios.2015.12.018

Cited by 14 publications

(3 citation statements)

References 49 publications

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“…Aequorin, a photoprotein, was recently used in conjunction with hybridoma technology for the first time in a proof-of-concept research on Vibrio cholerae diagnosis. The sensor allowed bacteria detection down to 50 CFU mL −1 within 7 seconds [215]. Krasitskaya's group recently developed a dual-aptamer solid-phase sandwich-type microassay in serum for the ultrasensitive detection (LOD: 6.3 nM) of autoantibodies to myelin basic protein, a [186]; (b) Vancomycin conjugated magnetic particles for gram-positive bacteria detection [187]; (c) Magnetic nanoliposomes for bioluminescent protein sensing [196]; (d) Magnetic sandwich assay for procalcitonin detection [197]; (e) Furimazine-based antibody detection using LUMABS [202]; (f) Obelin as a photoprotein in the sensing of anti-myelin basic protein autoantibody [210].…”

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

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Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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“…Поскольку технология CANARY в описанном варианте сложна для воспроизведения, то становится целесообразной ее модификация с целью сокращения трудозатрат и упрощения методологии создания эукариотического биосенсора, например, путем использования гибридом. Известно, что в данном направлении уже проводится работа коллективом авторов из Ирана [19]. Исследователи предлагают в качестве платформы для сенсора использовать гибридомы, секретирующие моноклональные антитела к О-антигену возбудителя холеры.…”

Section: биологическая безопасность и защита от биологических угрозunclassified

CANARY Technology: Working Principles of Biosensor, Detection of Infectious Pathogens

Gorshkov,

Pechenkin,

Kuznetsovskiy

2020

jour

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Reusable biosensors for the detection of biochemical analytes are widely used in clinical and laboratory practice. However, biosensors for the detection of pathogenic microorganisms are still under development or implementation. One of these devices is CANARY biosensor (Cellular Analysis and Notification of Antigen Risks and Yields), used by the US Army to indicate pathogenic biological agents. The aim of this article is to consider operating principles and molecular-biological foundations of CANARY biosensor, to analyze the possible directions of work and the prospects for creating domestically made biosensors based on eukaryotic cells. The concept of CANARY is that its receptor component is a B-lymphocyte, modified using genetic engineering, which carries specific IgM-like B-cell receptors on the surface of the cytoplasmic membrane. These cells are able to specifically recognize the target antigen and generate a photosignal through the aequorin protein. Currently, biosensors are already created for the detection of causative agents of plague (100–1000 CFU / ml), tularemia (100 CFU / ml), anthrax (100–500 spores / ml), smallpox (<500 CFU / ml), some toxins (ricin – 3 ng / ml, botulinum toxin – 16 pg / ml). They are based on the CANARY biosensor. Due to high sensitivity and specificity of this method, the relative simplicity and high speed of analysis of one sample, the possibility of analyzing aerosol samples, this technology should be considered as a promising basis for the creation of domestically made biological sensors to detect hazardous biological agents in biological samples, water, food, ecological samples and in aerosols. The deterioration of the global epidemic situation caused by the spread of various strains of SARS-CoV-2 makes sensors based on CANARY technology especially relevant. To create a domestic analogue of such a biosensor, close cooperation with scientific institutions that specialize in molecular genetics and manufacturers of laboratory equipment is required.

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Hybridoma as a specific, sensitive, and ready to use sensing element: a rapid fluorescence assay for detection of Vibrio cholerae O1

Zamani¹,

Sajedi²,

Hosseinkhani³

et al. 2016

Anal Bioanal Chem

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Over the last decade, isolation and purification of monoclonal antibodies, for diagnostic analysis, have been carried out using the hybridoma expression system. The present study describes a novel example of a detection system using hybridoma cells containing antibody against O1 antigen directly for V. cholerae diagnosis, which is a major health problem in many parts of the world, especially in developing countries. This method has advantages such as simplicity, ease of process, and it does not require manipulation of hybridoma cell. For this approach, an efficient amount of fluorescence calcium indicator, fura 2-AM, was utilized, which emitted light when the intracellular calcium concentration increased as result of antigen binding to specific antibody. More reliable results are obtained via this method and it is considerably faster than other methods, which has the response time of less than 45 s for detection of V. Cholerae O1. Also, the limit of detection was computed to be 50 CFU/mL (<13 CFU per assay). In addition, no significant responses were observed in the presence of other bacteria with specific hybridoma or other cell lines exposed to V. cholerae O1. Furthermore, this method was successfully applied to V. cholerae O1 detection in spiked environmental samples, including water and stool samples without any pretreatment. All results reveal that hybridoma cells can provide a valuable, simple, and ready to use tool for rapid detection of other pathogenic bacteria, toxins, and analytes.

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A luminescent hybridoma-based biosensor for rapid detection of V. cholerae upon induction of calcium signaling pathway

Cited by 14 publications

References 49 publications

Recent Progress in Optical Sensors for Biomedical Diagnostics

Recent Progress in Optical Sensors for Biomedical Diagnostics

CANARY Technology: Working Principles of Biosensor, Detection of Infectious Pathogens

Hybridoma as a specific, sensitive, and ready to use sensing element: a rapid fluorescence assay for detection of Vibrio cholerae O1

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