A low temperature bonding of quartz microfluidic chip for serum lipoproteins analysis

Zhuang, Gui-Sheng; Jin, Qinghui; Liu, Jing; Cong, Hui; Liu, Kangdong; Zhao, Jianlong; Yang, Mengsu; Wang, Huimin

doi:10.1007/s10544-006-9142-z

Cited by 17 publications

(14 citation statements)

References 31 publications

(23 reference statements)

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“…Our finding that bare silicon has a stronger inhibitory effect on the polymerase than SiO 2 (RBI = 2.2 × 10 2 versus RBI = 4.4 × 10 2 without BSA) is in line with the literature [1,2,4]. Surprisingly, SiO 2 quartz (RBI = 2.9 × 10 3 ), which already has been used in microfluidics [39][40][41], does not have as strong of an effect as silicon or silicon with a 560 nm layer of SiO 2 . Because SiO 2 has at least 12 crystalline forms [42] which may be produced by both oxidation or chemical reaction deposition, its inhibition properties need further investigation.…”

Section: Materials Inhibitory Effectsupporting

confidence: 91%

Inhibitory effect of common microfluidic materials on PCR outcome

Kodzius

Xiao

et al. 2012

Sensors and Actuators B: Chemical

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a b s t r a c tIn this study, we established a simple method for evaluating the PCR compatibility of various common materials employed when fabricating microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most cases, adding bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, although they noticeably interacted with the polymerase. We provide a simple method of performing PCR-compatibility testing of materials using inexpensive instrumentation that is common in molecular biology laboratories. Furthermore, our method is direct, being performed under actual PCR conditions with high temperature. Our results provide an overview of materials that are PCR-friendly for fabricating microfluidic devices. The PCR reaction, without any additives, performed best with pyrex glass, and it performed worst with PMMA or acrylic glue materials.

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Section: Materials Inhibitory Effectsupporting

confidence: 91%

Inhibitory effect of common microfluidic materials on PCR outcome

Kodzius

Xiao

et al. 2012

Sensors and Actuators B: Chemical

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“…Recently, we have demonstrated that a low-temperature bonding of quartz microfluidic chips for serum lipoproteins analysis [1], LDL and HDL lipoproteins in the serum were separated by using Tricine buffer with methylglucamine. We observed that the analysis of blood samples with microfluidic devices was difficult due to surface adsorption of blood components which resulted in adsorption of sample on the walls leading to change the surface properties of the microchannels.…”

Section: Introductionmentioning

confidence: 99%

Microchip‐based small, dense low‐density lipoproteins assay for coronary heart disease risk assessment

Wang

Jin

et al. 2008

Electrophoresis

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Small, dense low-density lipoprotein (sdLDL) has been accepted as an emerging cardiovascular risk factor, and there has been an increasing interest in analytical methods for sdLDL profiling for diagnosis. Serum sdLDL may be measured by different laboratory techniques, but all these methods are laborious, time-consuming, and costly. Recently, we have demonstrated that a low-temperature bonding of quartz microfluidic chips for serum lipoproteins analysis (Zhuang, G., Jin, Q., Liu, J., Cong, H. et al., Biomed. Microdevices 2006, 8, 255-261). In contrast to this previous study, we chose SDS as anionic surfactant to modify both lipoproteins and the channel surface to minimize lipoprotein adsorption and improve the resolution of lipoprotein separation. Two major LDL subclass patterns including large, buoyant LDL (lLDL), sdLDL, and high-density lipoprotein (HDL) were effectively separated with high reproducibility. RSD values of the migration time (min) and peak areas of standard LDL and HDL were 6.28, 4.02, 5.02, and 2.5%, respectively. Serum lipoproteins of 15 healthy subjects and 15 patients with coronary heart disease (CHD) were separated by microchip CE. No peaks of sdLDL were detected in serum samples of healthy subjects while sdLDL fractional peaks were observed in patients' entire serum samples. These results suggested that the microchip-based sdLDLs assay was a simple, rapid, and highly efficient technique and significantly improved the analysis of CHD risk factors.

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“…Following LIF detection, lamp-based approaches form the second largest group of optical detection techniques used for microchip separations. Several examples of lamp-based excitation using epifluorescence microscopes combined with photomultiplier tube (PMT) detection have been reported [79,124,125], from the high speed separation of trypsin inhibitor and BSA [80,124] to the separation of lowdensity and high-density lipoproteins [126] in the serum.…”

Section: Lamp-based Fluorescence Detectionmentioning

confidence: 99%

“…A low-temperature bonding method for microfabrication of quartz chips without the need for clean room conditions, and intermediate layer has been developed [126]. The reliability and performance of the fabricated chip were demonstrated through a complete separation of serum lipoproteins by CZE-LIF.…”

Section: Proteins From Biological Fluidsmentioning

confidence: 99%

Recent innovations in protein separation on microchips by electrophoretic methods

Peng¹,

Pallandre²,

Tran³

et al. 2007

Electrophoresis

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Microchips for analytical purposes have attracted great attention over the last 20 years. In the present review, we focus on the most recent development of microchips for electrophoretic separation of proteins. This review starts with a short recalling about the microchips covering the basic microchip layout for CE and the commercial chips and microchip platforms. A short paragraph is dedicated to the surface treatment of microchips, which is of paramount importance in protein analysis. One section is dedicated to on-line sample pretreatment in microchips and summarizes different strategies to pre-concentrate or to purify proteins from complex matrixes. Most of the common modes used for CE of proteins have already been adapted to the chip format, while multidimensional approaches are still in progress. The different routes to achieve detection in microchip are also presented with a special attention to derivatization or labeling of proteins. Finally, several recent applications are mentioned. They highlight the great potential of electrophoretic separations of proteins in numerous fields such as biological, pharmaceutical or agricultural and food analysis. A bibliography with 151 references is provided covering papers published from 2000 to the early 2007.

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A low temperature bonding of quartz microfluidic chip for serum lipoproteins analysis

Cited by 17 publications

References 31 publications

Inhibitory effect of common microfluidic materials on PCR outcome

Inhibitory effect of common microfluidic materials on PCR outcome

Microchip‐based small, dense low‐density lipoproteins assay for coronary heart disease risk assessment

Recent innovations in protein separation on microchips by electrophoretic methods

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