A low prevalence of mycobacteria in freshwater fish from water reservoirs, ponds and farms

Mrlík, V.; Slaný, Michal; Kubečka, Jan; Seďa, Jaromír; Nečas, Alois; Babák, Vladimír; Slaná, Iva; Kříž, P.; Pavlík, I.

doi:10.1111/j.1365-2761.2012.01369.x

Cited by 20 publications

(20 citation statements)

References 33 publications

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“…The same paper also reported the misidentification of the strain as a member of the M. tuberculosis complex by commercial probes (AccuProbe and INNO-LiPA Mycobacteria); such a finding has not been confirmed with other strains. One isolate from fish has also been reported (77).…”

Section: Mycobacterium Kumamotonensementioning

confidence: 99%

Microbiological Features and Clinical Relevance of New Species of the Genus Mycobacterium

2014

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SUMMARY Nontuberculous mycobacteria (NTM) are present in the environment, mainly in water, and are occasionally responsible for opportunistic infections in humans. Despite the fact that NTM are characterized by a moderate pathogenicity, the diseases caused by NTM at various body sites are increasing on a worldwide level. Among over 150 officially recognized NTM species, only two or three dozen are familiar to clinicians, and even to most microbiologists. In this paper, approximately 50 new species described in the last 8 years are reviewed, and their role in human infections is assessed on the basis of reported clinical cases. The small number of reports concerning most of the “new” mycobacterial species is responsible for the widespread conviction that they are very rare. Their role is actually largely underestimated, mainly because they often remain unrecognized and misidentified. Aiming to minimize such bias, emphasis has been placed on more common identification pitfalls. Together with new NTM, new members of the Mycobacterium tuberculosis complex described in the last few years are also an object of the present review.

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Section: Mycobacterium Kumamotonensementioning

confidence: 99%

Microbiological Features and Clinical Relevance of New Species of the Genus Mycobacterium

2014

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“…It is worth noting that the probability to find mycobacterial infections is higher in farmed fishes than in wild ones, as already pointed out in a recent study for several freshwater species [56]. M. chelonae is considered a human pathogen associated with infection of wound in immunocompromised person.…”

Section: Discussionmentioning

confidence: 94%

Mycobacterium chelonae associated with tumor-like skin and oral masses in farmed Russian sturgeons (Acipenser gueldenstaedtii)

et al. 2014

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BackgroundNon-tuberculous mycobacteria responsible for piscine mycobacteriosis usually produce visceral granulomas in both freshwater and marine species. In this study, the first occurrence of Mycobacterium chelonae associated with tumor-like lesions in the Russian sturgeon (Acipenser gueldenstaedtii) is reported. Fifteen sturgeons from an Italian fish farm showing skin and oral cauliflower-like masses were investigated by histopathology, bacterial culture and molecular analyses.ResultsA total of 20 masses different in size located in the mouth and in pectoral and caudal fins (characterized by abundant calcium deposits and by mild to moderate granulomatous inflammation) were observed with a significant different degree of histological severity. All internal organs of the fish were negative for mycobacteria, Ziehl-Neelsen was positive in only one of the oral masses, whereas bacterial and PCR analyses detected the presence of M. chelonae for almost all the skin and oral masses. Based on these results, a calcinosis of dystrophic origin associated with a chronic granulomatous inflammation was considered as a primary diagnosis consequent to tissue injury in areas susceptible to trauma.ConclusionsWe hypothesized that the occurrence of M. chelonae in farmed sturgeons was only a secondary event related to its presence in a stressful rearing environment and subsequent to a dystrophic calcinosis occurred in previously damaged tissues.

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“…, Danio sp. , Colisa sp., and Trichogaster species were reported in previous studies (Marzouk et al., ; Novotny et al., ; Peterson et al., ); however, only a few studies have been related to the incidence of mycobacteria in clinically healthy fish populations (Beran et al., ; Mrlik et al., ).…”

Section: Introductionmentioning

confidence: 80%

Identification of Mycobacterium species from apparently healthy freshwater aquarium fish using partial sequencing and PCR-RFLP analysis of heat shock protein (hsp65 ) gene

Shukla

Sharma

et al. 2014

J. Appl. Ichthyol.

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The present study analysed the incidence of mycobacteria in apparently healthy looking freshwater aquarium fish in Uttar Pradesh (State), India. Sixty fish belonging to eight different species were collected from six aquarium shops in different cities and processed for isolation of Mycobacterium species. Using the initial protocol of decontamination of tissue homogenates (with 1N HCl & 2N NaOH) and incubation at 30°C for 2 months, Mycobacterium sp. was isolated from 25% of the fish. The isolates were identified by standard biochemical tests. A 441 bp fragment of the hsp65 gene was amplified and digested by two fastdigest restriction enzymes, BstEII and HaeIII. Digested products were analysed using agarose gel electrophoresis. Sequencing of amplified fragments of the hsp65 gene was also performed. Isolates were identified as: five isolates of M. abscessus, three M. gordonae, two M. fortuitum, two M. conceptionense, two M. parascrofulaceum, and one isolate of M. senegalense. Mycobacterial incidence in apparently healthy looking freshwater aquarium fish is dreadful and the study is relevant because of the mycobacterial diversity related to aquarium fish and its zoonotic importance. All Mycobacterium species isolated in this study are well known pathogens in humans as well as fish.U.S.

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A low prevalence of mycobacteria in freshwater fish from water reservoirs, ponds and farms

Cited by 20 publications

References 33 publications

Microbiological Features and Clinical Relevance of New Species of the Genus Mycobacterium

Microbiological Features and Clinical Relevance of New Species of the Genus Mycobacterium

Mycobacterium chelonae associated with tumor-like skin and oral masses in farmed Russian sturgeons (Acipenser gueldenstaedtii)

Identification of Mycobacterium species from apparently healthy freshwater aquarium fish using partial sequencing and PCR-RFLP analysis of heat shock protein (hsp65 ) gene

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